# positions[].organizations[].parent[].label
College of Agriculture and Life Sciences

# overview
The use of molecular probes is allowing us to gain new insights into fungal plant pathogens and to host responses to potential pathogens. We are currently developing real-time PCR primers for two downy mildews that are considered to be a threat to maize production if introduced into the US. We are also developing PCR-based tags genes for resistance to headsmut, anthracnose, downy mildew and grain mold in sorghum. These molecular tags will be useful for breeding cultivars with more durable resistance and for cloning specific resistance genes. We have also used PCR to clone segments of the cotton and sorghum equivalents of genes that function in known host defense pathways. These clones are being used to compare the rate and timing of induction of each gene in resistant and susceptible lines following inoculation with a pathogen. Genome wide association studies are being used to identify genes associated with disease response (susceptible or resistant) to several pathogens in sorghum.

# keywords[]
Downy Mildew Detection And Identification

# keywords[]
Random Amplified Polymorphic Dna Technique

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
3002 Agriculture, Land And Farm Management

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Random Amplified Polymorphic Dna Technique

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# educationAndTraining[].organization.label
Cornell University - (Ithaca, New York, United States)

# publications[].label
DETECTION AND IDENTIFICATION OF PERONOSCLEROSPORA-SACCHARI IN MAIZE BY DNA HYBRIDIZATION

# publications[].label
Correlation of growth inhibition patterns to nucleoside transport models in Neurospora crassa.

# publications[].label
Guanine uptake and metabolism in Neurospora crassa.

# publications[].label
Characterization and genetic distance analysis of isolates of Peronosclerospora sorghi using AFLP fingerprinting.

# publications[].label
Bridging Classical and Molecular Genetics of Sorghum Disease Resistance

# publications[].label
Identification and characterization of RAPD and SCAR markers linked to anthracnose resistance gene in sorghum [Sorghum bicolor (L.) Moench]

# publications[].label
Adenosine kinase-deficient mutant of Neurospora crassa.

# publications[].label
Genetic and Pathogenic Variability among Isolates of Sporisorium reilianum Causing Sorghum Head Smut.

# publications[].label
DNABased Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum

# publications[].label
Elicitation of defense response genes in sorghum floral tissues infected by Fusarium thapsinum and Curvularia lunata at anthesis

# publications[].label
Effect of Leaf Age, Leaf Segments and Surface Treatments on Pathogenicity Levels of Colletotrichum sublineola in Sorghum and Johnson Grass

# publications[].label
Embryogenesis in callus derived from rice microspores.

# publications[].label
GWAS analysis of sorghum association panel lines identifies SNPs associated with disease response to Texas isolates of Colletotrichum sublineola.

# publications[].label
Use of a single sequencing termination reaction to distinguish between cytosine and 5-methylcytosine in bisulfite-modified DNA.

# publications[].label
A Genome-Wide Association Study of Nigerien and Senegalese Sorghum Germplasm of Exserohilum turcicum, the Causal Agent of Leaf Blight.

# publications[].label
PCR Amplification Following Restriction to Detect Site-Specific DNA Methylation

# publications[].label
Mating type a locus alleles and genomic polymorphism in Sporisorium reilianum: comparison of sorghum isolates to those from maize

# publications[].label
SPONTANEOUS MUTATIONS AT FINGERPRINT LOCI IN CLONAL LINEAGES OF THE RICE BLAST FUNGUS

# publications[].label
Stage-specific DNA methylation in a fungal plant pathogen.

# publications[].label
Expression of potential defense response genes in cotton

# publications[].label
Early events in the interaction between maize and Southern rust

# publications[].label
Mapping of verticillium wilt resistance genes in cotton

# publications[].label
A RFLP linkage map of Sorghum bicolor (L.) Moench.

# publications[].label
Specific PCR Detection of Fusarium oxysporum f. sp. vasinfectum California Race 4 Based on a Unique Tfo1 Insertion Event in the PHO Gene.

# publications[].label
Expression of pathogenesis-related protein PR-10 in sorghum floral tissues in response to inoculation with Fusarium thapsinum and Curvularia lunata.

# publications[].label
IMPROVED RATE OF CALLUS AND GREEN PLANT-PRODUCTION FROM RICE ANTHER CULTURE FOLLOWING COLD SHOCK

# publications[].label
The enzymatic cyclization of nerolidyl diphosphate by -cadinene synthase from cotton stele tissue infected with Verticillium dahliae

# publications[].label
Screening of exotic and commercial sorghum accessions against a new virulent race (P6) of Peronosclerospora sorghi causing downy mildew disease

# publications[].label
Defense responses against the sorghum anthracnose pathogen in leaf blade and midrib tissue of johnsongrass and sorghum

# publications[].label
Genome wide association analysis of sorghum mini core lines regarding anthracnose, downy mildew, and head smut.

# publications[].label
Downy mildews from gramineaceous crops in North America and those of regulatory concern

# publications[].label
Molecular mapping of Cg1, a gene for resistance to anthracnose (Colletotrichum sublineolum) in sorghum

# publications[].label
Heterokaryosis and parasexuality in Pyricularia oryzae Cavara.

# publications[].label
A Genome-Wide Association Study of Senegalese Sorghum Seedlings Responding to Pathotype 5 of Sporisorium reilianum.

# publications[].label
Genetic diversity among sorghum races and working groups based on AFLPs and SSRs

# publications[].label
IDENTIFICATION OF MOLECULAR MARKERS LINKED TO HEAD SMUT RESISTANCE GENE (SHS) IN SORGHUM BY RFLP AND RAPD ANALYSES

# publications[].label
Sorghum breeding for biotic stress tolerance

# publications[].label
IMPROVED RATE OF CALLUS AND GREEN PLANT-PRODUCTION FROM RICE ANTHER CULTURE FOLLOWING COLD-SHOCK

# publications[].label
Use of an A-T-rich DNA clone for identification and detection of Peronosclerospora sorghi.

# publications[].label
DNA methylation and Dc8-GUS transgene expression in carrot (Daucus carota L.).

# publications[].label
Reduced levels of cadinane sesquiterpenoids in cotton plants expressing antisense (+)-delta-cadinene synthase.

# publications[].label
Reaction of Cotton Cultivars and an F2 Population to Stem Inoculation with Isolates Verticillium dahliae

# publications[].label
Non-symmetrical and sustained strand-specific unequal methylation in the Dc8 gene of carrot

# publications[].label
Molecular tags for disease resistance genes in sorghum: Improved prospects for mapping

# publications[].label
Regulatory roles of abscisic acid for anthocyanin synthesis in maize kernels

# publications[].label
Leaf angle distribution in Johnsongrass, leaf thickness in sorghum and Johnsongrass, and association with response to Colletotrichum sublineola.

# publications[].label
A molecular marker that segregates with sorghum leaf blight resistance in one cross is maternally inherited in another.

# publications[].label
Agrobacterium wilt and bronzing: the new challenge

# publications[].label
Histidine uptake in strains of Neurospora crassa with normal and mutant transport systems.

# publications[].label
Evaluation of Gambian and Malian sorghum germplasm against downy mildew pathogen, Peronosclerospora sorghi, in Mexico and the USA

# publications[].label
Identification and characterization of RAPD and SCAR markers linked to anthracnose resistance gene in sorghum [Sorghum bicolor (L.) Moench] (vol 149, pg 179, 2006)

# publications[].label
The essentiality of sulfhydryl groups to transport in Neurospora crassa.

# publications[].label
Classification and characterization of the viviparous mutants of maize (Zea mays L.)

# publications[].label
Identification of RFLP markers linked to a gene for downy mildew resistance (Sdm) in sorghum

# publications[].label
Differential induction of 3-hydroxy-3-methylglutaryl CoA reductase in two cotton species following inoculation with Verticillium.

# publications[].label
Changes in soluble endosperm proteins associated with selection of quality protein maize lines

# publications[].label
Length heterogeneity in ITS 2 and the methylation status of CCGG and GCGC sites in the rRNA genes of the genus Peronosclerospora.

# publications[].label
Depression of uracil uptake by ammonium in Neurospora crassa.

# publications[].label
Multi-Trait Genome-Wide Association Studies of Sorghum bicolor Regarding Resistance to Anthracnose, Downy Mildew, Grain Mold and Head Smut.

# publications[].label
DNA methylation and expression of NPT II in transgenic petunias and progeny.

# publications[].label
The Grain Mold Pathogen, Fusarium thapsinum, Reduces Caryopsis Formation in Sorghum bicolor

# publications[].label
MITOCHONDRIAL-DNA (MTDNA) - RELATEDNESS AMONG FORMAE-SPECIALES OF FUSARIUM-OXYSPORUM IN THE CUCURBITACEAE

# publications[].label
Primers that amplify inserts in a multicloning site also hybridize to Sorghum bicolor DNA.

# publications[].label
Changes in nucleotide pools during conidial germination in Neurospora crassa.

# publications[].label
Terpenoid aldehyde formation and lysigenous gland storage sites in cotton: variant with mature glands but suppressed levels of terpenoid aldehydes.

# publications[].label
Regulation of hypoxanthine transport in Neurospora crassa.

# publications[].label
Purine base transport in Neurospora crassa.

# publications[].label
Diseases of Johnsongrass (Sorghum halepense): possible role as a reservoir of pathogens affecting other plants

# publications[].label
Genetic Diversity and Classification of Colletotrichum sublineola Pathotypes Using a Standard Set of Sorghum Differentials

# publications[].label
Virulence and molecular genotyping studies of Sporisorium relianum isolates in sorghum

# publications[].label
Simple sequence repeat markers useful for sorghum downy mildew (Peronosclerospora sorghi) and related species.

# publications[].label
The rice psb-A chloroplast gene has a standard location

# publications[].label
Genetic diversity and pathotype determination of Colletotrichum sublineolum isolated causing anthracnose disease in sorghum

# publications[].label
Role of purine base excretion in regulation of purine pools.

# publications[].label
Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola.

# publications[].label
A Sephadex column procedure for DNA isolation is also useful for detecting dsRNA.

# publications[].label
Histidine uptake in mutant strains of Neurospora crassa via the general transport system for amino acids.

# publications[].label
Stereoselective coupling of hemigossypol to form (+)-gossypol in moco cotton is mediated by a dirigent protein.

# publications[].label
An apparent case of nonsymmetrical and sustained strand-specific hemimethylation in the Dc8 gene of carrot.

# publications[].label
Elicitation of defense response genes in Sorghum bicolor (L.) Moench in response to infection by Fusarium thapsinum and Curvularia lunata at anthesis

# publications[].label
Virulence and Molecular Genotyping Studies of Sporisorium reilianum Isolates in Sorghum.

# publications[].label
Response of Senegalese Sorghum Seedlings to Pathotype 5 of Sporisorium reilianum

# publications[].label
Genome-Wide Association Study of Seed Morphology-Related TRAITS in Sorghum Mini Core and Senegalese Lines

# publications[].label
Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars.

# publications[].label
RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS - A SYSTEM FOR IDENTIFYING AND DIFFERENTIATING ISOLATES OF COLLETOTRICHUM-GRAMINICOLA

# publications[].label
Genetic dissection of the abscisic acid biosynthetic pathway in maize (Zea mays L.) embryos

# publications[].label
Changes in nucleotide pools during conidial germination in Neurospora crassa.

# publications[].label
Evaluation of the Ugandan sorghum accessions for grain mold and anthracnose resistance

# publications[].label
Reaction to Rust by a Subset of Sorghum Accessions from Zimbabwe

# publications[].label
Relationship between (8-14C)adenosine transport and growth inhibition in Neurospora crassa strain ad-8.

# publications[].label
Inoculation and Screening Methods for Major Sorghum Diseases Caused by Fungal Pathogens: Claviceps africana, Colletotrichum sublineola, Sporisorium reilianum, Peronosclerospora sorghi and Macrophomina phaseolina.

# publications[].label
SEED TRANSMISSION OF SORGHUM DOWNY MILDEW - DETECTION BY DNA HYBRIDIZATION

# publications[].label
A low-copy-number Sorghum DNA sequence that detects hypervariable EcoRV fragments.

# publications[].label
Chalcone synthase and phenylalanine ammonia-lyase mRNA levels following exposure of sorghum seedlings to three fungal pathogens

# publications[].label
Laser Capture Microdissection and Real-Time PCR for Measuring mRNA in Giant Cells Induced by Meloidogyne javanica.

# publications[].label
Transformation of plant pathogenic fungus, Fusarium oxysporum f sp niveum, to hygromycin B resistance and altered pathogenicity

# publications[].label
RFLP-based assay of Sorghum bicolor (L.) Moench genetic diversity.

# publications[].label
A region of maize chromosome 2 affects response to downy mildew pathogens.

# publications[].label
Genetic diversity and pathotype determination of Colletotrichum sublineolum isolates causing anthracnose in sorghum

# publications[].label
DNA markers for downy mildew resistance genes in sorghum.

# researcherOn[].label
Identification of Genes Involved in the Pathogenicity of Fusarium Oxysporum f.sp. Vasinfectum Race 4

# researcherOn[].awardedBy.label
Agricultural Research Service - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Molecular Approaches to Reducing Plant Diseases

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Genetic Analysis of Fusarium oxysporum f. sp. vasinfectum Race 4 Pathogenicity

# researcherOn[].awardedBy.label
Agricultural Research Service - (Washington D.C., District of Columbia, United States)

# streetAddress
Texas A&M University; Plant Pathology & Microbiology; 2132 TAMU

# schools[]
College of Agriculture and Life Sciences

# etdChairOf[].label
Cho, Jae-Min (2005-08). Isolation and characterization of resistance gene analogs (RGAs) in sorghum. Doctoral Dissertation.

# etdChairOf[].label
Viswanathan, Aparna (2004-09). Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2. Master's Thesis.

# etdChairOf[].label
Sabry, Ahmed Mohamed-Bashir (2004-09). QTL mapping of resistance to sorghum downy mildew in maize. Doctoral Dissertation.

# etdChairOf[].label
Katile, Seriba Ousmane (2009-05). Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene. Doctoral Dissertation.

# etdChairOf[].label
Radwan, Ghada Lotfy Hassan Elhefny (2013-02). Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance. Doctoral Dissertation.