# positions[].organizations[].parent[].label
Texas A&M University Naresh K. Vashisht College of Medicine

# overview
Our laboratory works with the obligate intracellular bacterial pathogen, Coxiella burnetii, the etiologic agent of Q fever and a category B biothreat agent. The long-term goal of this research is to understand the molecular pathogenic mechanisms involved in the host-pathogen interaction. To accomplish this broad goal, project in the lab are designed to test the molecular mechanisms employed by both the host and pathogen. Current pathogen studies include 1) broad survey of proteins secreted via a type 4 secretion system (T4SS) followed by determination of essentiality of each substrate for virulence and detailed analysis of mechanism of host modulation 2) survey of essential virulence loci identified by specific mutant screens, and 3) definition of the relative virulence of phylogenetically distinct isolate groups.

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
Centers For Disease Control And Prevention (cdc)

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
1.1 Normal Biological Development And Functioning

# educationAndTraining[].organization.label
Washington State University - (Pullman, Washington, United States)

# educationAndTraining[].organization.label
Miami University - (Oxford, Ohio, United States)

# educationAndTraining[].organization.label
Uniformed Services University of the Health Sciences - (Bethesda, Maryland, United States)

# educationAndTraining[].organization.label
Washington State University - (Pullman, Washington, United States)

# publications[].label
Direct cell extraction from fresh and stored soil samples: Impact on microbial viability and community compositions

# publications[].label
Isolation from animal tissue and genetic transformation of Coxiella burnetii are facilitated by an improved axenic growth medium.

# publications[].label
DNA probes for the identification of Coxiella burnetti strains.

# publications[].label
Constitutive SOS expression and damage-inducible AddAB-mediated recombinational repair systems for Coxiella burnetii as potential adaptations for survival within macrophages.

# publications[].label
Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II.

# publications[].label
Correlation of plasmid type and disease caused by Coxiella burnetii.

# publications[].label
Coxiella burnetii Intratracheal Aerosol Infection Model in Mice, Guinea Pigs, and Nonhuman Primates.

# publications[].label
Evaluation of Coxiella burnetii antibiotic susceptibilities by real-time PCR assay.

# publications[].label
Neural network based integration of assays to assess pathogenic potential.

# publications[].label
Edema disease as a model for systemic disease induced by Shiga toxin-producing E. coli.

# publications[].label
Profiling the humoral immune response of acute and chronic Q fever by protein microarray.

# publications[].label
Molecular pathogenesis of Coxiella burnetii in a genomics era.

# publications[].label
Modulation of innate immune signaling by a Coxiella burnetii eukaryotic-like effector protein.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Phylogenetic diversity, virulence and comparative genomics.

# publications[].label
Surfactant Protein D Binds to Coxiella burnetii and Results in a Decrease in Interactions with Murine Alveolar Macrophages.

# publications[].label
Identification of CD4+ T cell epitopes in C. burnetii antigens targeted by antibody responses.

# publications[].label
Coxiella effector protein CvpF subverts RAB26-dependent autophagy to promote vacuole biogenesis and virulence.

# publications[].label
System for the exposure of cell suspensions to power-frequency electric fields.

# publications[].label
Coxiella burnetii acid phosphatase inhibits the release of reactive oxygen intermediates in polymorphonuclear leukocytes.

# publications[].label
Immunogenicity and Reactogenicity in Q Fever Vaccine Development.

# publications[].label
Identification of three amino acid residues in the B subunit of Shiga toxin and Shiga-like toxin type II that are essential for holotoxin activity.

# publications[].label
Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Coxiella burnetii Whole Cell Vaccine Produces a Th1 Delayed-Type Hypersensitivity Response in a Novel Sensitized Mouse Model.

# publications[].label
Identification and characterization of arginine finger-like motifs, and endosome-lysosome basolateral sorting signals within the Coxiella burnetii type IV secreted effector protein CirA.

# publications[].label
Contrasting Lifestyles Within the Host Cell

# publications[].label
Comparative virulence of phase I and II Coxiella burnetii in immunodeficient mice.

# publications[].label
Phylogenetic diversity of the Rickettsiae.

# publications[].label
Differential expression of translational elements by life cycle variants of Coxiella burnetii.

# publications[].label
Developmental biology of Coxiella burnettii.

# publications[].label
Clinical and pathologic changes in a guinea pig aerosol challenge model of acute Q fever.

# publications[].label
Isolation and characterization of a plasmid from phase I Coxiella burnetii.

# publications[].label
Chemokine Receptor 7 Is Essential for Coxiella burnetii Whole-Cell Vaccine-Induced Cellular Immunity but Dispensable for Vaccine-Mediated Protective Immunity.

# publications[].label
Genome analysis of Coxiella burnetii species: insights into pathogenesis and evolution and implications for biodefense.

# publications[].label
Vaccine for immunization against Q-fever

# publications[].label
Cloning and porin activity of the major outer membrane protein P1 from Coxiella burnetii.

# publications[].label
Identification and cloning potentially protective antigens of Coxiella burnetii using sera from mice experimentally infected with Nine Mile phase I.

# publications[].label
Soluble antigens derived from Coxiella burnetii elicit protective immunity in three animal models without inducing hypersensitivity.

# publications[].label
Contrasting Lifestyles Within the Host Cell

# publications[].label
Laboratory maintenance of Coxiella burnetii.

# publications[].label
Identification of Coxiella burnetii type IV secretion substrates required for intracellular replication and Coxiella-containing vacuole formation.

# publications[].label
Modulation of the host transcriptome by Coxiella burnetii nuclear effector Cbu1314.

# publications[].label
Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella.

# publications[].label
A minimal set of DNA repair genes is sufficient for survival of Coxiella burnetii under oxidative stress.

# publications[].label
Proteome and antigen profiling of Coxiella burnetii developmental forms.

# publications[].label
Evidence that proteolytic separation of Shiga-like toxin type IIv A subunit into A1 and A2 subunits is not required for toxin activity.

# publications[].label
Cloning and sequencing of Coxiella burnetii outer membrane protein gene com1.

# publications[].label
The SCID Mouse Model for Identifying Virulence Determinants in Coxiella burnetii.

# publications[].label
Nanovaccines against Intracellular Pathogens Using Coxiella burnetii as a Model Organism

# publications[].label
Coxiella burnetii isolates cause genogroup-specific virulence in mouse and guinea pig models of acute Q fever.

# publications[].label
Global Reprogramming of Host Kinase Signaling in Response to Fungal Infection.

# publications[].label
T cells are essential for bacterial clearance, and gamma interferon, tumor necrosis factor alpha, and B cells are crucial for disease development in Coxiella burnetii infection in mice.

# publications[].label
Identification of Coxiella burnetii CD8+ T-Cell Epitopes and Delivery by Attenuated Listeria monocytogenes as a Vaccine Vector in a C57BL/6 Mouse Model.

# publications[].label
Stability of plasmid sequences in an acute Q-fever strain of Coxiella burnetii.

# publications[].label
Genomic polymorphisms as inherent watermarks for tracking infectious agents.

# publications[].label
Shiga toxin-producing Escherichia coli infection: temporal and quantitative relationships among colonization, toxin production, and systemic disease.

# publications[].label
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine.

# publications[].label
Candidate antigens for Q fever serodiagnosis revealed by immunoscreening of a Coxiella burnetii protein microarray.

# publications[].label
Preliminary assessment of genome differences between the reference Nine Mile isolate and two human endocarditis isolates of Coxiella burnetii.

# publications[].label
Characterization of a novel intratracheal aerosol challenge model of Brucella melitensis in guinea pigs

# publications[].label
Stimulation of toll-like receptor 2 by Coxiella burnetii is required for macrophage production of pro-inflammatory cytokines and resistance to infection.

# publications[].label
The emerging roles of long non-coding RNA in host immune response and intracellular bacterial infections.

# publications[].label
The Type IV Secretion System Effector Protein CirA Stimulates the GTPase Activity of RhoA and Is Required for Virulence in a Mouse Model of Coxiella burnetii Infection.

# publications[].label
The rickettsias and the bacteria formerly associated with the order Rickettsiales, namely, the genus Coxiella, are of major importance for the study of obligate intracellular microorganisms and their potential use as biological weapons. Preface.

# publications[].label
Intervention with Shiga toxin (Stx) antibody after infection by Stx-producing Escherichia coli.

# publications[].label
Developmental Cycle of Coxiella burnetii

# publications[].label
When outgroups fail; phylogenomics of rooting the emerging pathogen, Coxiella burnetii.

# publications[].label
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells.

# publications[].label
Genomic and Proteomic Approaches Against Q Fever

# publications[].label
Fur-regulated genes in Coxiella burnetii.

# publications[].label
Hepatitis associated with C. burnetii isolates.

# publications[].label
DNA watermarking of infectious agents: progress and prospects.

# publications[].label
Coxiella burnetii induces apoptosis during early stage infection via a caspase-independent pathway in human monocytic THP-1 cells.

# publications[].label
Coxiella burnetii inhibits host immunity by a protein phosphatase adapted from glycolysis.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Complete genome sequence of the Q-fever pathogen Coxiella burnetii.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE.

# publications[].label
Contrasting Lifestyles Within the Host Cell.

# publications[].label
Purified Shiga-like toxins induce expression of proinflammatory cytokines from murine peritoneal macrophages.

# publications[].label
Evaluation of the role of Shiga and Shiga-like toxins in mediating direct damage to human vascular endothelial cells.

# publications[].label
Binding of shiga toxin 2e to porcine erythrocytes in vivo and in vitro.

# publications[].label
Use of reverse vaccinology in the design and construction of nanoglycoconjugate vaccines against burkholderia pseudomallei

# publications[].label
Identification and cloning of immunodominant antigens of Coxiella burnetii.

# publications[].label
Interaction of Shiga toxins with human brain microvascular endothelial cells: cytokines as sensitizing agents.

# publications[].label
Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice.

# publications[].label
QUANTITATION AND LOCALIZATION OF SHIGA TOXIN SHIGA-LIKE TOXIN-BINDING GLYCOLIPID RECEPTORS IN HUMAN AND BABOON TISSUES

# publications[].label
Space: A Final Frontier for Vacuolar Pathogens.

# publications[].label
Characterization of the GDP-D-mannose biosynthesis pathway in Coxiella burnetii: the initial steps for GDP--D-virenose biosynthesis.

# publications[].label
Detection of tick-borne pathogens by MassTag polymerase chain reaction.

# publications[].label
Correction: Characterization of an intratracheal aerosol challenge model of Brucella melitensis in guinea pigs.

# publications[].label
Genomic analysis of phase I and II Coxiella burnetii with restriction endonucleases.

# publications[].label
Limited role for iron regulation in Coxiella burnetii pathogenesis.

# publications[].label
An experimental model for subclinical edema disease (Escherichia coli enterotoxemia) manifest as vascular necrosis in pigs.

# publications[].label
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

# publications[].label
Quantitative Yeast Genetic Interaction Profiling of Bacterial Effector Proteins Uncovers a Role for the Human Retromer in Salmonella Infection.

# publications[].label
An enzymatic mutant of Shiga-like toxin II variant is a vaccine candidate for edema disease of swine.

# publications[].label
Mutagenic and cytotoxic evaluation of Plutonium-244

# publications[].label
Age-related resistance to 987P fimbria-mediated colonization correlates with specific glycolipid receptors in intestinal mucus in swine.

# publications[].label
Characterization of a stress-induced alternate sigma factor, RpoS, of Coxiella burnetii and its expression during the development cycle.

# publications[].label
Both inducible nitric oxide synthase and NADPH oxidase contribute to the control of virulent phase I Coxiella burnetii infections.

# publications[].label
Characterization of an intratracheal aerosol challenge model of Brucella melitensis in guinea pigs.

# publications[].label
CYP1B1-AS1 regulates CYP1B1 to promote Coxiella burnetii pathogenesis by inhibiting ROS and host cell death.

# publications[].label
Defense mechanisms against oxidative stress in Coxiella burnetii: adaptation to a unique intracellular niche.

# publications[].label
Molecular pathogenesis of the obligate intracellular bacterium Coxiella burnetii.

# publications[].label
The specific activities of Shiga-like toxin type II (SLT-II) and SLT-II-related toxins of enterohemorrhagic Escherichia coli differ when measured by Vero cell cytotoxicity but not by mouse lethality.

# publications[].label
Identification and cloning of a 27-kDa Coxiella burnetii immunoreactive protein.

# publications[].label
A systematic approach to evaluate humoral and cellular immune responses to Coxiella burnetii immunoreactive antigens.

# publications[].label
Cloning, expression, and characterization of a Coxiella burnetii Cu/Zn Superoxide dismutase.

# publications[].label
Performance Standards for Biological Threat Agent Assays for Department of Defense Applications.

# publications[].label
Identification and characterization of an immunodominant 28-kilodalton Coxiella burnetii outer membrane protein specific to isolates associated with acute disease.

# publications[].label
Coxiella burnetii: Recent Advances and New Perspectives in Research of the Q Fever Bacterium Preface

# publications[].label
Immunoreactive Coxiella burnetii Nine Mile proteins separated by 2D electrophoresis and identified by tandem mass spectrometry.

# publications[].label
Mechanisms of vaccine-induced protective immunity against Coxiella burnetii infection in BALB/c mice.

# publications[].publisher
The American Association of Immunologists

# publications[].label
Comparison of the glycolipid receptor specificities of Shiga-like toxin type II and Shiga-like toxin type II variants.

# publications[].label
Tripping on acid: trans-kingdom perspectives on biological acids in immunity and pathogenesis.

# publications[].label
Reduction in microbial infection risks from raw milk by Electron Beam Technology

# publications[].label
Subunit Vaccines Using TLR Triagonist Combination Adjuvants Provide Protection Against Coxiella burnetii While Minimizing Reactogenic Responses.

# publications[].label
Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Intratracheal Inoculation with Brucella melitensis in the Pregnant Guinea Pig Is an Improved Model for Reproductive Pathogenesis and Vaccine Studies.

# publications[].label
Genetic diversity of the Q fever agent, Coxiella burnetii, assessed by microarray-based whole-genome comparisons.

# publications[].label
Primary Murine Macrophages as a Tool for Virulence Factor Discovery in Coxiella burnetii.

# publications[].label
Mapping the minimal contiguous gene segment that encodes functionally active Shiga-like toxin II.

# publications[].label
Vaccination with genetically modified Shiga-like toxin IIe prevents edema disease in swine.

# publications[].label
Special Issue: Selected papers from the 7th International Conference on the Foundations of Computer-Aided Process Design (FOCAPD, 2009), Breckenridge, Colorado, USA - Preface

# publications[].label
The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion.

# researcherOn[].label
Identification and Role of Type IV Secretion Effector Proteins in Coxiella Burnetii

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].awardedBy.label
United States Department of Defense - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
The immunomodulatory role of Ankyrin repeat containing effectors expressed by Coxiella burnetii

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
Transposon and Targeted Mutational Analysis of Coxiella Burnetii To Identify Virulence Determinants of Acute and Chronic Diseases

# researcherOn[].awardedBy.label
United States Department of Defense - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
iSENTRY: An Integrated Microfluidics-Enabled System for Phenotypic Detection of Biothreat Agents

# researcherOn[].awardedBy.label
United States Department of Defense - (Washington D.C., District of Columbia, United States)

# streetAddress
Texas A&M Health Science Center; Microbial Pathogenesis And Immunology; 1114 TAMUS

# organizations[]
Texas A&M University Naresh K. Vashisht College of Medicine

# schools[]
Texas A&M University Naresh K. Vashisht College of Medicine

# inTheNews[].label
WATCH NOW: COVID-19 The Texas A&M System Responds - Episode 12

# inTheNews[].label
A&M experts offer updates on COVID-19 vaccine testing on-campus

# etdChairOf[].label
Rowin, Kristina D (2014-12). Identifying Coxiella burnetii Genes Essential for Subversion of the Host Immune System. Master's Thesis.

# etdChairOf[].label
Weber, Mary (2014-05). Identification of C. burnetii Type IV Secretion Substrates Required for Intracellular Replication and Coxiella-Containing Vacuole Formation. Doctoral Dissertation.

# etdChairOf[].label
Hong, Priscilla Christine (2005-08). Utilization of the persistent nature of Brucella in the development of live vaccines. Doctoral Dissertation.

# etdChairOf[].label
Russell-Lodrigue, Kasi Elizabeth (2006-12). An inhalation model of acute Q fever in guinea pigs. Doctoral Dissertation.