# positions[].organizations[].parent[].label
College of Veterinary Medicine and Biomedical Sciences

# overview
The overarching research goal of the Yang laboratory is to understand the mechanisms governing viral replication, with the rationale that the discoveries will expand the knowledge of both viruses and their hosts, and facilitate the development of novel strategies to combat viral and non-viral diseases. A parallel goal of Yang lab is to provide a highly supportive environment to train the next generations of scientists. The ongoing research focuses on how viruses interact with two cellular housekeeping processes: protein synthesis and metabolism using vaccinia virus as the research model. Vaccinia virus is the prototype poxvirus. Poxviruses significantly impact public health, with many presently causing morbidity and mortality in humans and many economically important animals, including deadly zoonotic pathogens (e.g., monkeypox virus). In addition, despite the eradication of smallpox, one of the most (if not the most) devastating diseases in human history, smallpox resurgence remains a serious biothreat. Poxviruses are also widely developed as veterinary and human vaccine vectors and as cancer treatment agents. Poxviruses provide numerous precious tools to understand many aspects of cell biology and dissect complex life processes, as their large DNA genomes encode hundreds of genes that engage many key nodes of cellular life. Yang's research integrates biochemical, molecular, and omics  approaches. Taking advantage of their in-depth knowledge of the poxvirus replication and virus-host interactions, the Yang lab also develops vaccinia virus-based utilities and anti-virals.

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
Mechanistic Target Of Rapamycin Complex 1

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
Internal Ribosome Entry Site Trans‑acting Factor

# keywords[]
3404 Medicinal And Biomolecular Chemistry

# educationAndTraining[].organization.label
University of Nebraska–Lincoln - (Lincoln, United States)

# publications[].label
Sample Preparation for Global Metabolic Profiling of Vaccinia Virus-Infected Primary Human Foreskin Fibroblasts.

# publications[].label
La-related protein 4 is enriched in vaccinia virus factories and is required for efficient viral replication in primary human fibroblasts.

# publications[].label
Vaccinia growth factor-dependent modulation of the mTORC1-CAD axis upon nutrient restriction.

# publications[].label
A poxvirus decapping enzyme localizes to mitochondria to regulate RNA metabolism and translation, and promote viral replication

# publications[].label
RPFdb: a database for genome wide information of translated mRNA generated from ribosome profiling.

# publications[].label
Future State of Smallpox Medical Countermeasures

# publications[].label
Viral growth factor- and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection.

# publications[].label
Drosophila S2 cells are non-permissive for vaccinia virus DNA replication following entry via low pH-dependent endocytosis and early transcription.

# publications[].label
Poxvirus-encoded decapping enzymes promote selective translation of viral mRNAs.

# publications[].label
The replication and transcription activator (RTA) of Kaposis sarcoma-associated herpesvirus/human herpesvirus-8

# publications[].label
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

# publications[].label
Genome-wide analysis of the 5' and 3' ends of vaccinia virus early mRNAs delineates regulatory sequences of annotated and anomalous transcripts.

# publications[].label
Alteration in Cellular Signaling and Metabolic Reprogramming during Viral Infection.

# publications[].label
Anticancer Drug Camptothecin Test in 3D Hydrogel Networks with HeLa cells.

# publications[].label
Enterovirus 71 3C Promotes Apoptosis through Cleavage of PinX1, a Telomere Binding Protein.

# publications[].label
Suppression of poxvirus replication by SC144.

# publications[].label
MVA titration by plaque assay using crystal violet staining in DF-1 cells

# publications[].label
Mpox (Monkeypox) Virus and Its Co-Infection with HIV, Sexually Transmitted Infections, or Bacterial Superinfections: Double Whammy or a New Prime Culprit?

# publications[].label
Progress in Structure, Function and Application of Promoters in the Nisin Gene Cluster

# publications[].label
Monkeypox emergency: Urgent questions and perspectives.

# publications[].label
La-related protein 4 is enriched in vaccinia virus factories and is required for efficient viral replication in primary human fibroblasts.

# publications[].label
Ciclopirox suppresses poxvirus replication by targeting iron metabolism.

# publications[].label
Role of vaccinia virus growth factor in stimulating the mTORC1-CAD axis of the de novo pyrimidine pathway under different nutritional cues.

# publications[].label
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells.

# publications[].label
Kaposi's sarcoma-associated herpesvirus transactivator RTA promotes degradation of the repressors to regulate viral lytic replication.

# publications[].label
Asparagine availability is an essential limiting factor for poxvirus protein synthesis

# publications[].label
A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication.

# publications[].label
Suppression of Poxvirus Replication by Resveratrol.

# publications[].label
Upregulation of ATP Citrate Lyase Phosphorylation and Neutral Lipid Synthesis through Viral Growth Factor Signaling during Vaccinia Virus Infection

# publications[].label
Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling.

# publications[].label
Enhancement of autophagy during lytic replication by the Kaposi's sarcoma-associated herpesvirus replication and transcription activator.

# publications[].label
Identification of Vaccinia Virus Inhibitors and Cellular Functions Necessary for Efficient Viral Replication by Screening Bioactives and FDA-Approved Drugs.

# publications[].label
Vaccinia Virus Transcriptome Analysis by RNA Sequencing.

# publications[].label
Expression profiling of the intermediate and late stages of poxvirus replication.

# publications[].label
Antiviral activities of two nucleos(t)ide analogs against vaccinia and mpox viruses in primary human fibroblasts.

# publications[].label
Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts.

# publications[].label
Viral Reprogramming of Nucleotide Synthesis and Its Impact on Viral Infection.

# publications[].label
Discovery of Small-Molecule Orthopoxvirus Resolvase Inhibitors with Antiviral Activity.

# publications[].label
Modified Vaccinia Virus Ankara Titration Using Crystal Violet- or Immuno-Staining in DF-1 Cells.

# publications[].label
Influence of growth conditions on the stability of plasmid-like DNAs from cucumber mitochondria

# publications[].label
Stimulation of neutral lipid synthesis via viral growth factor signaling and ATP citrate lyase during vaccinia virus infection.

# publications[].label
Simultaneous high-resolution analysis of vaccinia virus and host cell transcriptomes by deep RNA sequencing.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Identification of the internal ribosome entry sites inthe 5'untranslated region of the cfos gene.

# publications[].label
Deciphering viral infections by ribosome profiling.

# publications[].publisher
Editorial Office of Chinese Journal of Virology

# publications[].label
The Role of Tape Measure Protein in Nucleocytoplasmic Large DNA Virus Capsid Assembly.

# publications[].label
The action of S1 Nuclease and a cloning strategy for microcircular DNAs

# publications[].label
Asparagine: An Achilles Heel of Virus Replication?

# publications[].label
Going against the Tide: Selective Cellular Protein Synthesis during Virally Induced Host Shutoff.

# publications[].label
Molluscum Contagiosum Virus Transcriptome in Abortively Infected Cultured Cells and a Human Skin Lesion.

# publications[].label
1-Hydroxy-1,8-napthyridinone (DHN) Analogs Potently Inhibit Monkeypox Virus Resolvase (Mpr).

# publications[].label
Rapid and quantitative evaluation of vaccinia virus-induced host shutoff using newly generated cell lines stably expressing secreted Gaussia luciferase.

# publications[].label
Ribosome Profiling Reveals Translational Upregulation of Cellular Oxidative Phosphorylation mRNAs during Vaccinia Virus-Induced Host Shutoff.

# publications[].label
Discovery and Characterization of a Hydroxypyridone-3-carboxamide Analog as an Antiviral Lead against Orthopoxviruses.

# publications[].label
Isolation and identification of plasmid-like DNAs in Cucumber mitochondria

# publications[].label
The transcriptional repressor K-RBP modulates RTA-mediated transactivation and lytic replication of Kaposi's sarcoma-associated herpesvirus.

# publications[].label
Ciclopirox suppresses poxvirus replication by targeting iron metabolism.

# publications[].label
Asparagine Is a Critical Limiting Metabolite for Vaccinia Virus Protein Synthesis during Glutamine Deprivation.

# publications[].label
Simultaneous and systematic analysis of cellular and viral gene expression during Enterovirus 71-induced host shutoff.

# publications[].label
The zinc finger DNA-binding domain of K-RBP plays an important role in regulating Kaposi's sarcoma-associated herpesvirus RTA-mediated gene expression.

# publications[].label
The 5'-poly(A) leader of poxvirus mRNA confers a translational advantage that can be achieved in cells with impaired cap-dependent translation.

# publications[].label
Temporal expression classes and functions of vaccinia virus and mpox (monkeypox) virus genes.

# publications[].label
An urgent need for diagnostic tools to address global mpox public health emergencies.

# publications[].label
Vaccinia Virus Capping Enzyme Subunit D12 Is Differentially Required for Viral Replication in Different Cell Lines and Gastrointestinal Organoids.

# publications[].label
Pervasive initiation and 3'-end formation of poxvirus postreplicative RNAs.

# publications[].label
Inhibitors of One or More Cellular Aurora Kinases Impair the Replication of Herpes Simplex Virus 1 and Other DNA and RNA Viruses with Diverse Genomes and Life Cycles.

# publications[].label
Vaccinia Virus as a Master of Host Shutoff Induction: Targeting Processes of the Central Dogma and Beyond.

# publications[].label
Cascade regulation of vaccinia virus gene expression is modulated by multistage promoters.

# publications[].label
Ribosome Profiling of Vaccinia Virus-Infected Cells.

# publications[].label
Interaction of the vaccinia virus RNA polymerase-associated 94-kilodalton protein with the early transcription factor.

# editorOf[].label
Vaccinia, Mpox, and Other Poxviruses Methods and Protocols

# organizations[]
College of Veterinary Medicine and Biomedical Sciences

# organizations[]
"College of Veterinary Medicine and Biomedical Sciences"@en-US

# schools[]
College of Veterinary Medicine and Biomedical Sciences

# schools[]
"College of Veterinary Medicine and Biomedical Sciences"@en-US