# positions[].organizations[].parent[].label
School of Veterinary Medicine and Biomedical Sciences

# overview
My laboratory is interested in developing transgenic animal models of disease and novel platforms for the production of biopharmaceuticals. We are currently exploring methods to produce vaccines in the milk of transgenic animals.

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

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Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Insulin-like Growth Factor Binding Proteins

# educationAndTraining[].organization.label
Kansas State University - (Manhattan, Kansas, United States)

# educationAndTraining[].organization.label
Texas A&M University - Corpus Christi - (Corpus Christi, Texas, United States)

# educationAndTraining[].organization.label
Texas A&M University - (College Station, Texas, United States)

# publications[].label
Rescuing valuable genomes by animal cloning: a case for natural disease resistance in cattle.

# publications[].label
Bovine nuclear transfer embryo development using cells derived from a cloned fetus.

# publications[].label
Expression of lactate dehydrogenase mRNAs in bovine pre-attachment embryos.

# publications[].label
Effects of low temperatures on in vitro produced bovine zygotes.

# publications[].label
Assessment by differential display-RT-PCR of mRNA transcript transitions and -amanitin sensitivity during bovine preattachment development

# publications[].label
Analysis of DNA (cytosine 5) methyltransferase mRNA sequence and expression in bovine preimplantation embryos, fetal and adult tissues.

# publications[].label
Production of in-vitro derived blackbuck antelope (Antilope cervicapra) embryos using standard bovine protocols

# publications[].label
A simple salt solution medium supplemented with yolk plasma and lactate (YPLM) supports development of preimplantation bovine embryos in vitro.

# publications[].label
The effect of donor cell serum starvation and oocyte activation compounds on the development of somatic cell cloned embryos.

# publications[].label
Effect of co-culture with theca interna on nuclear maturation of horse oocytes with low meiotic competence, and subsequent fusion and activation rates after nuclear transfer.

# publications[].label
Three little pigs worth the huff and puff?

# publications[].label
Reprogramming gene expression following nuclear transfer into bovine oocytes.

# publications[].label
Development of in vitro derived bovine embryos following pronuclear transplantation and in vitro culture.

# publications[].label
Advances in large-animal reproduction: The expanding role of ultrasonography

# publications[].label
Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses.

# publications[].label
Response to comments on "Early pregnancy diagnosis by palpation per rectum: Influence on embryo/fetal viability in dairy cattle" by Juan E. Romano, et al. [Theriogenology 67 (2007) 486-493]

# publications[].label
Early pregnancy diagnosis by transrectal ultrasonography in dairy cattle.

# publications[].label
Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development.

# publications[].label
Effect of cooling temperatures on pre-compacted bovine embryos

# publications[].label
Cyclooxygenase-2 and prostaglandin E(2)(PGE(2)) receptor messenger RNAs are affected by bovine oocyte maturation time and cumulus-oocyte complex quality, and PGE(2) induces moderate expansion of the bovine cumulus in vitro.

# publications[].label
In vitro production of axis deer (axis axis) embryos, a preliminary study

# publications[].label
Histone-lysine N-methyltransferase SETDB1 is required for development of the bovine blastocyst.

# publications[].label
Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization.

# publications[].label
Viable embryos and normal calves after nuclear transfer into Hoechst stained enucleated demi-oocytes of cows.

# publications[].label
Blastocyst development of in vitro produced morula-stage bovine embryos briefly exposed to Ultraviolet light following Hoechst staining.

# publications[].label
Nuclear transplantation and cloning in mammals.

# publications[].label
Characterization of a bovine cDNA encoding citrate synthase, and presence of citrate synthase mRNA during bovine pre-attachment development

# publications[].label
Interspecific transfer of IVM IVF-derived red sheep (Ovis orientalis gmelini ) embryos to domestic sheep (Ovis aries ).

# publications[].label
Advances in large-animal reproduction: Updated techniques for artificial insemination and embryo transfer

# publications[].label
The effects of heparin exposure on equine oocytes matured in-vitro

# publications[].label
Production of transgenic calves expressing an shRNA targeting myostatin.

# publications[].label
MICROINJECTION OF SPERMATOZOA INTO BOVINE EGGS

# publications[].label
Effects of low temperatures on in vitro produced bovine zygotes

# publications[].label
Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos

# publications[].label
Genetic engineering a sheep model of Hypophosphatasia.

# publications[].label
Production of cloned blastocysts using epithelial cells cultured from bovine semen

# publications[].label
Isolation and Genetic Transformation of Primordial Germ Cell (PGC)- Derived Cells from Cattle, Goats, Rabbits and Rats

# publications[].publisher
Asian Australasian Association of Animal Production Societies

# publications[].label
Assessment by differential display-RT-PCR of mRNA transcript transitions and alpha-amanitin sensitivity during bovine preattachment development.

# publications[].label
Production of nuclear transfer horse embryos by Piezo-driven injection of somatic cell nuclei and activation with stallion sperm cytosolic extract.

# publications[].label
FERTILIZATION OF HAMSTER OVA BY SPERM INJECTION - THE EFFECT OF SPERM PLACEMENT ON SPERM DECONDENSATION AND PRONUCLEAR FORMATION

# publications[].label
Temporal patterns of embryonic gene expression and their dependence on oogenetic factors.

# publications[].label
Embryo production and possible species preservation by nuclear transfer of somatic cells isolated from bovine semen.

# publications[].label
Transgenic sheep generated by lentiviral vectors: safety and integration analysis of surrogates and their offspring.

# publications[].label
Clinical and molecular characterization of a re-established line of sheep exhibiting hemophilia A.

# publications[].label
In vitro maturation of ovine oocytes in a portable incubator.

# publications[].label
Characterization of differentially expressed mRNAs in bovine pre-attachment embryos cultured in defined and serum-supplemented/co-culture systems

# publications[].label
Genetic engineering a large animal model of human hypophosphatasia in sheep.

# publications[].label
An egg yolk fraction high in LDL supports in vitro development of bovine embryos in a cell-free basic salt solution supplemented with lactate.

# publications[].label
Characterization of a bovine cDNA encoding citrate synthase, and presence of citrate synthase mRNA during bovine pre-attachment development.

# publications[].label
Activation of equine nuclear transfer oocytes: methods and timing of treatment in relation to nuclear remodeling.

# publications[].label
New commercial opportunities for advanced reproductive technologies in horses, wildlife, and companion animals.

# publications[].label
Inhibition of bovine fertilization in vitro by antibodies to SP-10.

# publications[].label
Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Oxygen-induced alterations in the expression of chromatin modifying enzymes and the transcriptional regulation of imprinted genes.

# publications[].label
Characterization of PTHrP and PTHr in the bovine ovary.

# publications[].label
Genetic reprogramming of lactate dehydrogenase, citrate synthase, and phosphofructokinase mRNA in bovine nuclear transfer embryos produced using bovine fibroblast cell nuclei

# publications[].label
EMBRYOS PRODUCED BY THE TRANSFER OF CAPRINE NUCLEI TO ENUCLEATED BOVINE OOCYTES ARE CAPABLE OF CLEAVAGE BUT DO NOT DEVELOP TO BLASTOCYSTS

# publications[].label
Effects of early pregnancy diagnosis by palpation per rectum on pregnancy loss in dairy cattle.

# publications[].publisher
American Veterinary Medical Association (AVMA)

# publications[].label
Cloning of GJA1 (connexin43) and its expression in canine ovarian follicles throughout the estrous cycle.

# publications[].label
Conservation of germ plasm from bison infected with Brucella abortus.

# publications[].label
Genetically Engineering a Sheep Model of Hypophosphatasia

# publications[].label
Evaluation of culture systems for attachment and proliferation of epithelial cells cultured from ovine semen.

# publications[].label
Gene expression regulating blastocyst formation.

# publications[].label
Assessment of telomerase activity and telomere length in cloned bovine embryos, fetuses and offspring derived by somatic cell nuclear transfer

# publications[].label
Amino acid concentrations in fluids from the bovine oviduct and uterus and in KSOM-based culture media.

# publications[].label
Levels of amino acids in bovine oviductal and uterine fluids in comparison to levels available in a culture medium supplemented with commercial stocks

# publications[].label
Advances in large-animal reproduction: In vitro production of embryos

# publications[].label
Use of Piezo-driven direct nuclear injection and activation with stallion sperm extract to produce horse nuclear transfer embryos

# publications[].label
The effect of mercaptoethanol and N-acetyl cysteine on development of bovine blastocysts produced in vitro.

# publications[].label
Reshaping the transcriptional frontier: epigenetics and somatic cell nuclear transfer.

# publications[].label
Suppression of prion protein in livestock by RNA interference.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Effects of oocyte culture density on meiotic competence of canine oocytes.

# publications[].label
Effect of low temperatures on in-vitro matured bovine oocytes.

# publications[].label
Nuclear transfer in the bovine embryo: a comparison of 5-day, 6-day, frozen-thawed, and nuclear transfer donor embryos.

# publications[].label
COMPARISON OF BIRTH-WEIGHT AND GROWTH-CHARACTERISTICS OF BOVINE CALVES PRODUCED BY NUCLEAR TRANSFER (CLONING), EMBRYO-TRANSFER AND NATURAL MATING

# publications[].label
Effect of procedures utilized for nuclear transfer on bovine oocyte activation

# publications[].label
Early pregnancy diagnosis by palpation per rectum: influence on embryo/fetal viability in dairy cattle.

# publications[].label
A cat cloned by nuclear transplantation.

# publications[].label
DNA hypomethylation of karyoplasts for bovine nuclear transplantation.

# publications[].label
Genetic reprogramming of phosphofructokinase in nuclear transfer embryos produced using bovine fetal fibroblasts.

# publications[].label
PRODUCTION OF IDENTICAL BOVINE OFFSPRING BY NUCLEAR TRANSFER

# publications[].label
Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis.

# publications[].label
In vitro development of equine nuclear transfer embryos: effects of oocyte maturation media and amino acid composition during embryo culture.

# publications[].label
Early pregnancy diagnosis by palpation per rectum on embryo/fetus viability in dairy cattle

# publications[].label
The role of cyclooxygenase (COX-2), prostaglandin E2 (PGE2) and prostaglandin receptors 2 and 3 (EP2 and EP3) during maturation in vitro of bovine cumulus oocyte complexes (COCs).

# publications[].label
Embryo cloning in cattle: the use of in vitro matured oocytes.

# publications[].label
Expression of PTHrP and PTHR (PTH/PTHrP-r) mRNAs and polypeptides in bovine ovary and stimulation of bovine blastocyst development in vitro following PTHrP treatment during oocyte maturation.

# publications[].label
Role of cumulus cells on in vitro maturation of canine oocytes.

# publications[].label
High blastocyst development following nuclear transfer with fibroblast cells derived from a cloned fetus.

# publications[].label
Reprogramming of fibroblast nuclei after transfer into bovine oocytes.

# publications[].label
Regenerated bovine fetal fibroblasts support high blastocyst development following nuclear transfer.

# publications[].label
Assessment of canine oocyte viability after transportation and storage under different conditions.

# publications[].label
Cloning companion animals (horses, cats, and dogs).

# publications[].label
Reducing the amount of cytoplasm available for early embryonic development decreases the quality but not quantity of embryos produced by in vitro fertilization and nuclear transplantation.

# publications[].label
Early pregnancy diagnosis by transrectal ultrasonography in dairy cattle (vol 66, pg 1034, 2006)

# publications[].label
Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos.

# publications[].label
Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro.

# publications[].label
The effect of NaCl-associated osmolarity changes on bovine embryo development in a completely defined culture medium supplemented with amino acids at oviductal fluid levels.

# publications[].label
Monoclonal antibody to human fertilization antigen-1 (FA-1) inhibits bovine fertilization in vitro: application in immunocontraception.

# publications[].label
Culture of agar-embedded one and two cell bovine embryos and embryos produced by nuclear transfer in the sheep and rabbit oviduct

# publications[].label
Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells.

# publications[].label
Cloning of exotic/endangered species: desert bighorn sheep.

# publications[].label
Sensitivity of bovine blastocyst gene expression patterns to culture environments assessed by differential display RT-PCR.

# publications[].label
Genetic reprogramming of lactate dehydrogenase, citrate synthase, and phosphofructokinase mRNA in bovine nuclear transfer embryos produced using bovine fibroblast cell nuclei

# publications[].label
Lentivirus mediated transgenic livestock: applications in biomedical research

# publications[].label
In vitro development of bovine embryos in a BRL coculture system supplemented with EGG yolk in the presence or absence of fetal bovine serum and bovine serum albumin.

# publications[].label
IGF paracrine and autocrine interactions between conceptus and oviduct.

# publications[].label
Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies).

# publications[].label
Influence of recipient oocyte cell cycle stage on DNA synthesis, nuclear envelope breakdown, chromosome constitution, and development in nuclear transplant bovine embryos.

# publications[].label
Serum starvation of bovine fetal fibroblasts prior to nuclear transfer increases in vitro development rates.

# researcherOn[].label
Functional Analysis of the Embryonic Epigenome In A Non-Rodent Model - Supplemental Account

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].label
Development of New Approaches for Genetic Modification of Livestock

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Inducible Tissue-Specific Transgene Expression In Large Animal Biomedical Models

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-Office of Director-Office of Research Infrastructure Programs

# researcherOn[].label
Functional Anaylsis of the Embryonic Epigenome In A Non-Rodent Model

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# streetAddress
Texas A&M University; Vet Med - Physiology & Pharmacology; 4466 TAMU

# schools[]
School of Veterinary Medicine and Biomedical Sciences

# inTheNews[].label
Texas A&M Vet School Announces The Death Of The World's First Cloned Cat

# inTheNews[].label
CC, the world's first cloned cat, passes away in College Station

# etdChairOf[].label
Liu, Jie (2009-05). Recovery and evaluation of somatic cells from ovine and bovine semen for use in nuclear transfer. Doctoral Dissertation.

# etdChairOf[].label
Willingham-Rocky, Lauri A. (2009-05). Evaluation of oocyte competency in bovine and canine species via non-invasive assessment of oocyte quality. Doctoral Dissertation.

# etdChairOf[].label
Peoples, Michael D 1978- (2010-12). Identification and Characterization of Bovine Pol III Promoters to Express a Short-Hairpin RNA. Master's Thesis.

# etdChairOf[].label
Tessanne, Kimberly J. (2012-02). Development of Transgenic Livestock with Reduced Myostatin Expression Using RNA Interference. Doctoral Dissertation.

# etdChairOf[].label
Campbell, Scott Robert (2016-12). Development and Evaluation of an Oral Contraceptive for Wild Pigs. Master's Thesis.

# etdChairOf[].label
Golding, Michael Cameron (2005-02). Expression of the bovine DNA (cytosine 5) methyltransferase family during preimplantation development and aberrations induced by somatic cell nuclear transfer. Doctoral Dissertation.