# positions[].organizations[].parent[].label
College of Agriculture and Life Sciences

# overview
We combine approaches in molecular genetics, structural biology, biochemistry, proteomics, and bioinformatics to study the amazing RNA biology of trypanosome parasites.  One research line is on an RNA editing process by uridine insertion and deletion that creates amino acid coding triplets in most mRNAs. Yet a single error in the U-changes yields a frame-shift. Trypanosomes split from other eukaryotic lineages over a hundred million years ago, yet this editing has analogies with RNAi, CRISPR/Cas9, mRNA splicing and other systems directed by small non-coding RNAs (ncRNAs).

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Single-Strand Specific DNA And RNA Endonucleases

# educationAndTraining[].organization.label
National Autonomous University of Mexico - (Mexico City, Distrito Federal, Mexico)

# educationAndTraining[].organization.label
National Autonomous University of Mexico - (Mexico City, Distrito Federal, Mexico)

# educationAndTraining[].organization.label
London School of Hygiene & Tropical Medicine - (London, Camden, United Kingdom)

# publications[].label
Trypanosome RNA helicase KREH2 differentially controls non-canonical editing and putative repressive structure via a novel proposed 'bifunctional' gRNA in mRNA A6.

# publications[].label
Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei.

# publications[].label
Preferential interaction of a 25kDa protein with an A6 pre-mRNA substrate for RNA editing in Trypanosoma brucei.

# publications[].label
REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding.

# publications[].label
Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.

# publications[].label
T. brucei RNA editing: action of the U-insertional TUTase within a U-deletion cycle.

# publications[].label
Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria.

# publications[].label
Complete cycles of bloodstream trypanosome RNA editing in vitro.

# publications[].label
KREH2 helicase represses ND7 mRNA editing in procyclic-stage Trypanosoma brucei by opposite modulation of canonical and 'moonlighting' gRNA utilization creating a proposed mRNA structure.

# publications[].label
ProteinProtein and RNAProtein Interactions in UInsertion/Deletion RNA Editing Complexes

# publications[].label
Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition.

# publications[].label
A novel transcribed repeat element from Entamoeba histolytica.

# publications[].label
Roles for ligases in the RNA editing complex of Trypanosoma brucei: band IV is needed for U-deletion and RNA repair.

# publications[].label
Dynamic RNA holo-editosomes with subcomplex variants: Insights into the control of trypanosome editing.

# publications[].label
Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing.

# publications[].label
REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes.

# publications[].label
Peptidomimetic Vinyl Heterocyclic Inhibitors of Cruzain Effect Antitrypanosomal Activity.

# publications[].label
Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica.

# publications[].label
The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing.

# publications[].label
Ribonuclease activities of trypanosome RNA editing complex directed to cleave specifically at a chosen site.

# publications[].label
Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes.

# publications[].label
Substrate determinants for RNA editing and editing complex interactions at a site for full-round U insertion.

# publications[].label
Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes

# publications[].label
T. brucei RNA editing: adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage.

# publications[].label
RNA-protein interactions in assembled editing complexes in trypanosomes.

# publications[].label
Molecular and cell biology of opportunistic infections in AIDS. Entamoeba histolytica.

# publications[].label
Unexpected electrophoretic migration of RNA with different 3' termini causes a RNA sizing ambiguity that can be resolved using nuclease P1-generated sequencing ladders.

# publications[].label
A DNA probe specific to pathogenic Entamoeba histolytica.

# publications[].label
DEAH-RHA helicaseZnf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes.

# publications[].label
Resolution of the RNA editing gRNA-directed endonuclease from two other endonucleases of Trypanosoma brucei mitochondria.

# publications[].label
Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Guide to the nomenclature of kinetoplastid RNA editing: a proposal.

# publications[].label
Cloning, characterization and expression of two Xenopus bcl-2-like cell-survival genes.

# publications[].label
Direct sizing of RNA fragments using RNase-generated standards.

# publications[].label
Determinants for association and guide RNA-directed endonuclease cleavage by purified RNA editing complexes from Trypanosoma brucei.

# publications[].label
Distinct functions of two RNA ligases in active Trypanosoma brucei RNA editing complexes.

# publications[].label
RNA editing complex interactions with a site for full-round U deletion in Trypanosoma brucei.

# publications[].label
Trypanosome RNA editing: simple guide RNA features enhance U deletion 100-fold.

# publications[].label
Trypanosoma brucei U insertion and U deletion activities co-purify with an enzymatic editing complex but are differentially optimized.

# publications[].label
RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reaction.

# publications[].label
Stunning Intricacies of RNA Editing Complexes RECC, RESC, and REH2C: Functional Organization, Developmental Regulation, and Evolutionary History in Kinetoplastid Protists.

# publications[].label
Trypanosoma brucei RNA editing: coupled cycles of U deletion reveal processive activity of the editing complex.

# publications[].label
Minimal pre-mRNA substrates with natural and converted sites for full-round U insertion and U deletion RNA editing in trypanosomes.

# researcherOn[].label
Protozoan Purine Phosphoribosyltransferases as Targets to Treat Malaria, African Trypanosomiasis and Chagas's Disease

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
Protozoan Purine Phosphoribosyltransferases as Targets to Treat Malaria, African Trypanosomiasis and Chagas's Disease

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
Protozoan Purine Phosphoribosyltransferases as Targets to Treat Malaria, African Trypanosomiasis and Chagas's Disease

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
Collaborative Research: RNA processing in trypanosomes mitochondria

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
RNA Processing in Trypanosome Mitochondria

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Novel Inhibitors of Cruzain and Trypanosoma brucei Cathepsin B as Potential Drug Candidates for the treatment of African Trypanosomiasis and Chagas Disease

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# streetAddress
Texas A&M University; Biochemistry & Biophysics; 2128 TAMU

# organizations[]
College of Agriculture and Life Sciences

# schools[]
College of Agriculture and Life Sciences

# etdChairOf[].label
Hernandez, Alfredo J. (2010-12). RNA Editing in Trypanosomes: Substrate Recognition and its Integration to RNA Metabolism. Doctoral Dissertation.

# etdChairOf[].label
Kumar, Vikas (2016-05). The Function of a Central RNA Helicase RNP in Editosomes of Trypanosomes. Doctoral Dissertation.