# title
Resveratrol Phenocopies the Suppressive Effects of Insulin-like Growth Factor Receptor-1 siRNA on IGF-1 Promoted Colon Cancer Cell Proliferation

# abstract
<jats:p>The insulinlike growth factor (IGF) system is believed to mediate obesityrelated increase in the risk of colon cancer. IGFs activate the catenin pathway, which plays a central role in the growth of colon cancer cells. Dietary resveratrol exerts inhibitory effects on cancers; however, its molecular mechanisms are not fully elucidated, particularly in relation to obesitypromoted cancers. We hypothesized that resveratrol suppresses colon cancer cell proliferation by inhibiting activation of IGF receptor1 (IGF1R) in response to IGFs (elevated during obesity). IGF1 (10 nM) promoted proliferation of colon cancer cells, <jats:italic>in vitro</jats:italic>, as expected. Resveratrol (50150 M) or IGF1R siRNA (100 nM) inhibited (p < 0.05) proliferation of colon cancer cells in response to both exogenous and autocrine IGFs. The relative levels of IGF1R were reduced (p < 0.05) in cells treated with either resveratrol or IGF1R siRNA, in association with a loss (p < 0.05) in Akt activation, GSK3 phosphorylation, and cyclin D1 levels. These results suggest that resveratrol effectively phenocopies the inhibitory properties of IGF1R siRNA and may thus be a potent chemopreventive/therapeutic agent against obesityassociated increase in colon cancer risk. We will examine this possibility in future studies using <jats:italic>in vivo</jats:italic> models. Funded by TAES and AICR 058094.</jats:p>

# authors[].organizations[].label
College of Agriculture and Life Sciences

# authorList[]
Vanamala, J., Reddivari, L., Tarver, C. C., Murano, P. S., Lupton, J. R., & Singh, P.