# positions[].organizations[].parent[].label
Texas A&M University Naresh K. Vashisht College of Medicine

# overview
My laboratory works in two research areas. First, we are combining biophysical and genetic approaches to understand how proteins use unstructured regions to sense cellular information and respond by adjusting their function. We developed methods to purify Ultrabithorax, a full-length, active Hox transcription factor, and have used this unique opportunity to investigate the role of intramolecular regulatory interactions in tissue-specific protein regulation. Second, we discovered methods to self-assemble Ultrabithorax into robust, extensible materials that are biocompatible and provide unique opportunities for functionalization. These materials are being developed for use as biosensors and to pattern and instruct vascularization in tissue engineering scaffolds.

# keywords[]
3403 Macromolecular And Materials Chemistry

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Sterol Regulatory Element Binding Proteins

# keywords[]
3403 Macromolecular And Materials Chemistry

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# educationAndTraining[].organization.label
University of Illinois at Urbana Champaign - (Urbana, Illinois, United States)

# educationAndTraining[].organization.label
Rice University - (Houston, Texas, United States)

# educationAndTraining[].organization.label
University of North Carolina at Chapel Hill - (Chapel Hill, North Carolina, United States)

# awardsAndHonors[].label
Faculty Early Career Development (CAREER) Program

# publications[].label
Detection and prevention of protein aggregation before, during, and after purification.

# publications[].label
Multiple intrinsically disordered sequences alter DNA binding by the homeodomain of the Drosophila hox protein ultrabithorax.

# publications[].label
The Transcription Factor Ultrabithorax Forms Extensible, Hierarchically Ordered Assemblies that are Readily Functionalized by Gene Fusion

# publications[].label
Generating Novel Materials Using the Intrinsically Disordered Protein Ubx.

# publications[].label
Functionalization and Patterning of Protein-Based Materials Using Active Ultrabithorax Chimeras

# publications[].label
The critical role of intrinsic disorder in creating bioactive materials

# publications[].label
Context-dependent HOX transcription factor function in health and disease.

# publications[].label
Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic.

# publications[].label
Roles for intrinsic disorder and fuzziness in generating context-specific function in Ultrabithorax, a Hox transcription factor.

# publications[].label
Functionalized protein-based materials and their uses

# publications[].label
Dynamic protein-DNA recognition: beyond what can be seen.

# publications[].label
Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein.

# publications[].label
The Drosophila transcription factor ultrabithorax self-assembles into protein-based biomaterials with multiple morphologies.

# publications[].label
Evolution of the activation domain in a Hox transcription factor.

# publications[].label
Mechanically-Tunable, Protein-Based Materials Can be Functionalized with Other Proteins and with DNA

# publications[].label
-Lactoglobulin Enhances Clay and Activated Carbon Binding and Protection Properties for Cadmium and Lead.

# publications[].label
On the roles of intrinsically disordered proteins and regions in cell communication and signaling.

# publications[].label
Methods for measuring protein aggregation

# publications[].label
Measuring Hox-DNA binding by electrophoretic mobility shift analysis.

# publications[].label
Evolution of the Intrinsically Disordered Activation Domain in a Hox Transcription Factor

# publications[].label
Identifying solubility-promoting buffers for intrinsically disordered proteins prior to purification.

# publications[].label
Reverse Regulation: Controlling Intrinsically Disordered Domains with Structured Elements

# publications[].label
Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox Proteins.

# publications[].label
Physical and genetic interactions link hox function with diverse transcription factors and cell signaling proteins.

# publications[].label
Internal regulatory interactions determine DNA binding specificity by a Hox transcription factor.

# publications[].label
Immobilization and enhancement of a heterodimeric fluorescence biosensor in fibrous protein biomaterials.

# publications[].label
Dna delivery to cells by ultabithorax protein-based materials

# publications[].label
Ultrabithorax, an Intrinsically Disordered Protein, Selects Protein Interactions by Topology

# publications[].label
The Effect of Protein Fusions on the Production and Mechanical Properties of Protein-Based Materials

# publications[].label
Materials composed of the Drosophila melanogaster protein ultrabithorax are cytocompatible.

# publications[].label
Culture of Tumorigenic Cells on Protein Fibers Reveals Metastatic Cell Behaviors.

# publications[].label
Chemical and temporal manipulation of early steps in protein assembly tunes the structure and intermolecular interactions of protein-based materials.

# publications[].label
The intrinsically disordered regions of the Drosophila melanogaster Hox protein ultrabithorax select interacting proteins based on partner topology.

# publications[].label
Binding, Release, and Detection of Ligands by Ultrabithorax ProteinBased Materials

# publications[].label
Computationally Guided Rational Design of LOO-GFP Biosensors and Biosensor Materials

# publications[].label
CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila.

# publications[].label
Media composition influences yeast one- and two-hybrid results.

# publications[].label
Transcription activation by ultrabithorax Ib protein requires a predicted alpha-helical region.

# publications[].label
Identification of multiple dityrosine bonds in materials composed of the Drosophila protein Ultrabithorax.

# publications[].label
Recombinant human immunodeficiency virus type 1 reverse transcriptase is heterogeneous.

# publications[].label
Intrinsically disordered proteins play diverse roles in cell signaling.

# publications[].label
Team-teaching a current events-based biology course for nonmajors.

# publications[].label
Size dictates mechanical properties for protein fibers self-assembled by the Drosophila hox transcription factor ultrabithorax.

# publications[].label
Rethinking gene regulatory networks in light of alternative splicing, intrinsically disordered protein domains, and post-translational modifications.

# publications[].label
Identifying solubility-promoting buffers for intrinsically proteins prior to purification

# publications[].label
Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and non-immunogenic

# publications[].label
Combinatorial transcriptional regulation: the interaction of transcription factors and cell signaling molecules with homeodomain proteins in Drosophila development.

# publications[].label
Genetic Functionalization of Protein-Based Biomaterials via Protein Fusions.

# publications[].label
Engineering ubx-based materials to promote and guide neovascularization

# publications[].label
The Effect of Protein Fusions on the Production and Mechanical Properties of Protein-Based Materials

# publications[].label
Challenges and Solutions for Leave-One-Out Biosensor Design in the Context of a Rugged Fitness Landscape

# publications[].label
Separating full-length protein from aggregating proteolytic products using filter flow-through purification.

# publications[].label
Shape-Shifting to Regulate and Diversify Transcription Factor Function

# publications[].label
Functionalization of Ultrabithorax Materials with Vascular Endothelial Growth Factor Enhances Angiogenic Activity.

# publications[].label
What's in a name? Why these proteins are intrinsically disordered: Why these proteins are intrinsically disordered.

# publications[].label
On the design of composite protein-quantum dot biomaterials via self-assembly.

# publications[].label
Variations on a theme: Hox and Wnt combinatorial regulation during animal development.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# researcherOn[].label
I-Corps: High Affinity And High Specificity Ligand Binding Materials"

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Immobilization and Stabilization of Biocatalysts for Efficient Pharmaceutical Manufacturing

# researcherOn[].awardedBy.label
National Aeronautics and Space Administration - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Career: Using Functionalized Protein-Based Materials To Control and Pattern Cell Behavior

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# streetAddress
Texas A&M Health Science Center; Molecular And Cellular Medicine; 1114 TAMUS

# organizations[]
Texas A&M University Naresh K. Vashisht College of Medicine

# schools[]
Texas A&M University Naresh K. Vashisht College of Medicine

# etdChairOf[].label
Tsai, Shang-Pu (2016-05). Ubx as a Novel Protein-Based Material: Structural Insights, Functionalization via Gene Fusion and Biomedical Applications. Doctoral Dissertation.

# etdChairOf[].label
Hsiao, Hao-Ching (2015-05). Protein Interactions in Regulation and Assembly. Doctoral Dissertation.

# etdChairOf[].label
Schultz, Robbie (2015-05). From Signaling to Structure: Regulation of Extracellular Matrix in C. elegans. Doctoral Dissertation.