# positions[].organizations[].parent[].label
College of Agriculture and Life Sciences

# overview
My laboratory is broadly interested in understanding the structural basis of signal transduction events that occur at the membrane surface. These events are mediated by signaling proteins that reversibly associate with membranes in response to binding second messengers, such as Ca2+ ions, diacylglycerol, and phosphoinositides. One of the key kinases regulating these signal transduction pathways is the Protein Kinase C (PKC) family. Aberrant levels of PKC expression or activity have been implicated in a large number of human diseases, such as cancer, cardiac failure, Alzheimer's disease, and diabetes. Despite the significance of PKC in signal transduction and human health, the structural and dynamical basis of its activation upon binding to lipid membranes remains elusive.

# keywords[]
5 Development Of Treatments And Therapeutic Interventions

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# educationAndTraining[].organization.label
Columbia University - (New York, New York, United States)

# awardsAndHonors[].label
Faculty Early Career Development (CAREER) Program

# awardsAndHonors[].label
Ralph E. Powe Junior Faculty Enhancement Award

# publications[].label
Partial Metal Ion Saturation of C2 Domains Primes Synaptotagmin 1-Membrane Interactions.

# publications[].label
Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity?

# publications[].label
Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity?

# publications[].label
Expression and purification of the N-terminal regulatory domain of Protein Kinase C for biophysical studies.

# publications[].label
The structural basis of iron sensing by the human F-box protein FBXL5.

# publications[].label
Structural insights into C1-ligand interactions: Filling the gaps by in silico methods.

# publications[].label
Assignments of carbon NMR resonances for microcrystalline ubiquitin.

# publications[].label
Structural Basis of Agonist Capture by Regulatory Cl Domain of PKC

# publications[].label
Value of a hydrogen bond in triosephosphate isomerase loop motion.

# publications[].label
Structural basis of agonist capture by PKC-regulatory C1 domain

# publications[].label
Formation and photocatalytic properties of Q-sized nanoparticles of various transition metal sulphides in the inner cavities of lecithin vesicles modified with sodium dodecylsulphate

# publications[].label
Backbone and side chain dynamics of mutant calmodulin-peptide complexes.

# publications[].label
Toggling of Diacylglycerol Affinity Correlates with Conformational Plasticity in C1 Domains.

# publications[].label
Off-resonance TROSY-selected R 1rho experiment with improved sensitivity for medium- and high-molecular-weight proteins.

# publications[].label
Assignment of the backbone resonances for microcrystalline ubiquitin.

# publications[].label
Cd2+ as a Ca2+ surrogate in protein-membrane interactions: isostructural but not isofunctional.

# publications[].label
Deletion of the Trichoderma virens NRPS, Tex7, induces accumulation of the anti-cancer compound heptelidic acid.

# publications[].label
Sec14-nodulin proteins and the patterning of phosphoinositide landmarks for developmental control of membrane morphogenesis.

# publications[].publisher
American Society for Cell Biology (ASCB)

# publications[].label
Comparative studies of phase-cycling schemes for multiple pi-pulse sequences.

# publications[].label
Regulation of eukaryotic protein kinases by Pin1, a peptidyl-prolyl isomerase.

# publications[].label
Probing the determinants of diacylglycerol binding affinity in the C1B domain of protein kinase C.

# publications[].label
The C-terminal V5 domain of Protein Kinase C is intrinsically disordered, with propensity to associate with a membrane mimetic.

# publications[].label
High affinity interactions of Pb2+ with synaptotagmin I.

# publications[].label
Toxic Metal Ions Promote Self-Association and Replace Structural Zinc Ions in the Regulatory Region of Protein Kinase C

# publications[].label
High affinity interactions of Pb2+ with Synaptotagmin I

# publications[].label
Detection of the Pin1-ligand interactions using 19F NMR spectroscopy

# publications[].label
A novel bivalent interaction mode underlies a non-catalytic mechanism for Pin1-mediated protein kinase C regulation.

# publications[].label
Dynamic Response of the C2 Domain of Protein Kinase C to Ca2+ Binding.

# publications[].label
Probing the Diacylglycerol Binding Site of Presynaptic Munc13-1.

# publications[].label
Structural basis of double-stranded RNA recognition by the RIG-I like receptor MDA5.

# publications[].label
Mechanism of diacylglycerol sensing revealed by integrative structural biology approach

# publications[].label
Time-resolved solid-state REDOR-edited NMR detection of a transient enzyme-intermediate

# publications[].label
Characterization of chemical exchange using residual dipolar coupling.

# publications[].label
Reactive cysteine in the structural Zn(2+) site of the C1B domain from PKC.

# publications[].label
Non-Native Metal Ion Reveals the Role of Electrostatics in Synaptotagmin 1-Membrane Interactions.

# publications[].label
Not Just Ionic Mimicry: Biophysics of Toxic Metal Ion Interactions with Peripheral Membrane Targets

# publications[].label
Improvement of resolution in solid state NMR spectra with J-decoupling: an analysis of lineshape contributions in uniformly 13C-enriched amino acids and proteins.

# publications[].label
Partial metal ion saturation of C2 domains primes Syt1-membrane interactions

# publications[].label
Homo-nuclear 13C J-decoupling in uniformly 13C-enriched solid proteins.

# publications[].label
Cd(II)- and Pb(II)-Induced Self-Assembly of Peripheral Membrane Domains from Protein Kinase C.

# publications[].label
Structural and Kinetic Characterization of High-Affinity Lead(II)-Synaptotagmin I Interactions

# publications[].label
Structural Basis of Protein Kinase C Regulation by the C-Terminal Tail.

# publications[].label
Characterization of the fast dynamics of protein amino acid side chains using NMR relaxation in solution.

# publications[].label
Dynamics and Membrane Interactions of Protein Kinase C.

# publications[].label
Structural anatomy of Protein Kinase C C1 domain interactions with diacylglycerol and other agonists.

# publications[].label
Changes in calmodulin main-chain dynamics upon ligand binding revealed by cross-correlated NMR relaxation measurements.

# publications[].label
Synergistic effect of Pb(2+) and phosphatidylinositol 4,5-bisphosphate on C2 domain-membrane interactions.

# publications[].label
New strategies for combating fungal infections: Inhibiting inositol lipid signaling by targeting Sec14 phosphatidylinositol transfer proteins.

# publications[].label
Interfacial partitioning of a loop hinge residue contributes to diacylglycerol affinity of conserved region 1 domains.

# publications[].label
Conditional Membrane Proteins: Solution NMR Studies of Structure, Dynamics, and Function

# publications[].label
Pb2+ as modulator of protein-membrane interactions.

# publications[].label
Reactivity of Thiol-Rich Zn Sites in Diacylglycerol-Sensing PKC C1 Domain Probed by NMR Spectroscopy.

# publications[].label
PHOTOSTIMULATED VECTORIAL ELECTRON-TRANSFER ACROSS THE BILAYER-MEMBRANE OF LIPID VESICLES IN A SYSTEM WITH CDS NANOPARTICLES AS PHOTOSENSITIZER AND 1,4-BIS(1,2,6-TRIPHENYL-4-PYRIDYL) BENZENE AS A REVERSIBLE 2-ELECTRON CARRIER

# publications[].label
Lipid Membrane Influences Interaction between the C1 Domain of Munc13-1 and the Activator

# researcherOn[].label
Structural and functional studies of protein kinase C regulation

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# researcherOn[].label
CAREER: Biological Chemistry of Ph2+ Revealed Through Ph2+ Mediated Protein-Membrane Interactions

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Mechanisms of signal transduction revealed through unique chemistry of xenobiotic metal ions

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# streetAddress
Texas A&M University; Biochemistry & Biophysics; 2128 TAMU

# organizations[]
College of Agriculture and Life Sciences

# schools[]
College of Agriculture and Life Sciences

# etdChairOf[].label
Morales-Rivera, Krystal A. (2013-12). The Metal-dependent Function of C2α: A Conditional Membrane Domain from Protein Kinase Cα. Doctoral Dissertation.