# positions[].organizations[].parent[].label
College of Veterinary Medicine and Biomedical Sciences

# overview
My laboratory is currently working on a number of projects involving genetic engineering in cattle, goats, sheep and horses. We use CRISPR/Cas gene editing to specifically alter the coding sequence of genes in sheep to produced biomedical models of human disease, specifically hypophosphatasia.  My lab is actively working on projects to produce gene edited cattle that are resistant to respiratory disease. We have also successfully used gene editing to correct the glycogen branching enzyme deficiency mutation in horses. We are also interested in altering the carcass characteristics of beef cattle by genetic engineering genes specifically related to meat tenderness in Bos indicus cattle. Other projects in the lab involve the use of mesenchymal stem cell-based therapies for treatment of equine disease and in particular methods for using these cells to over express proteins that can modulate the inflammatory response. We also have interest in using livestock as bioreactors to produce biotherapeutics and vaccine antigens in their milk. I have extensive experience in using genetic engineering in combination with assisted reproductive technologies (including somatic cell nuclear transfer) to produce live animals.

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Clustered Regularly Interspaced Short Palindromic Repeats

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# educationAndTraining[].organization.label
University of Missouri - (Columbia, Missouri, United States)

# educationAndTraining[].organization.label
University of Missouri - (Columbia, Missouri, United States)

# educationAndTraining[].organization.label
University of Massachusetts Amherst - (Amherst Center, Massachusetts, United States)

# educationAndTraining[].organization.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# publications[].label
Genetic engineering a large animal model of human hypophosphatasia in sheep.

# publications[].label
Genetically Engineering a Sheep Model of Hypophosphatasia

# publications[].label
Birth of normal piglets after cytoskeletal stabilization of embryos and cryopreservation by vitrification

# publications[].label
DEVELOPMENT OF rLENTIVIRAL VECTORS FOR MALE GERMLINE-SPECIFIC Cre RECOMBINASE EXPRESSION IN LIVESTOCK

# publications[].label
Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses.

# publications[].label
Suppression of prion protein in livestock by RNA interference.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression.

# publications[].label
Initiation and organization of events during the first cell cycle in mammals: applications in cloning.

# publications[].label
Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Inactivation of histone H1 kinase by Ca2+ in rabbit oocytes.

# publications[].label
187 RE-ESTABLISHMENT OF AN EXTINCT STRAIN OF SHEEP FROM A LIMITED SUPPLY OF FROZEN SEMEN

# publications[].label
Assessment of canine oocyte viability after transportation and storage under different conditions.

# publications[].label
434 REPLICATION COMPETENT LENTIVIRUS (RCL) ANALYSIS IN RECIPIENT ANIMALS OF TRANSGENIC EMBRYOS PRODUCED BY LENTIVIRAL TRANSFER

# publications[].label
35ANALYSIS OF EPIGENETIC MODIFICATIONS AND GENOMIC IMPRINTING IN NUCLEAR TRANSFER DERIVED BOS GAURUSB. TAURUS CONCEPTI

# publications[].label
LASER ASSISTED HATCHING IN BOVINE IN VITRO PRODUCED EMBRYOS TO IMPROVE PREGNANCY RATES

# publications[].label
170 POST-THAW VIABILITY OF IN VITRO -PRODUCED BOVINE EMBRYOS CULTURED EITHER IN HOST CAPRINE REPRODUCTIVE TRACTS OR IN VITRO

# publications[].label
INHIBITION OF FOOT AND MOUTH DISEASE VIRUS IN VITRO USING RNA INTERFERENCE

# publications[].label
Production of the First True Model of Progressive Pseudorheumatoid Arthropathy of Childhood (PPAC) in Sheep

# publications[].label
Selective Inhibition of Prostaglandin E2 Receptors EP2 and EP4 Inhibits Growth of Experimental Endometriosis in Nude Mice Evaluated by Real-time Noninvasive Imaging and Fluorescence Microscopy.

# publications[].label
Production of transgenic calves expressing an shRNA targeting myostatin.

# publications[].label
The relationship between membrane status and fertility of boar spermatozoa after flow cytometric sorting in the presence or absence of seminal plasma.

# publications[].label
Morphology and subsequent development in culture of bovine oocytes matured in vitro under various conditions of fertilization.

# publications[].label
Sustained expression of insulin by a genetically engineered sertoli cell line after allotransplantation in diabetic BALB/c mice.

# publications[].label
4 SUPPRESSION OF ASH2L ALTERS DNA METHYLATION AND HISTONE PATTERNS DURING BOVINE EMBRYONIC DEVELOPMENT

# publications[].label
Maternal nutrient restriction alters endocrine pancreas development in fetal heifers.

# publications[].label
Dual labeling of the cytoskeleton and DNA strand breaks in porcine embryos produced in vivo and in vitro.

# publications[].label
Genetically engineered immune privileged Sertoli cells: A new road to cell based gene therapy.

# publications[].label
162 DEVELOPMENT AND EVALUATION OF AN ORAL CONTRACEPTIVE BAIT FOR FERAL PIGS

# publications[].label
Isolation and characterization of MPM-2-reactive sperm proteins: homology to components of the outer dense fibers and segmented columns.

# publications[].label
5 PRODUCTION OF TRANSGENIC LIVESTOCK USING A LENTIVIRUS EXPRESSING MULTIPLE SHORT INTERFERING RNAs TARGETING FOOT AND MOUTH DISEASE VIRUS

# publications[].label
141 BOVINE EMBRYO DEVELOPMENT RATES ARE AFFECTED WHEN OOCYTES ARE MATURED IN DIFFERENT VIALS CONTAINING HEPES/BICARBONATE BUFFERED MEDIUM

# publications[].label
Embryo production and possible species preservation by nuclear transfer of somatic cells isolated from bovine semen.

# publications[].label
Engineering bone phenotypes in domestic animals: Unique resources for enhancing musculoskeletal research.

# publications[].label
Review: Initiation and organization of events during the first cell cycle in mammals: Applications in cloning

# publications[].label
Clinical and molecular characterization of a re-established line of sheep exhibiting hemophilia A.

# publications[].label
DNA methylation-independent growth restriction and altered developmental programming in a mouse model of preconception male alcohol exposure.

# publications[].label
Factors involved in nuclear reprogramming during early development in the rabbit.

# publications[].label
CHARACTERIZATION OF LENTIVIRAL SHORT-HAIRPIN RNA EXPRESSION VECTORS CONTAINING SINGLE OR MULTIPLE BOVINE POLYMERASE III PROMOTERS

# publications[].label
Down-regulation of viral replication by lentiviral-mediated expression of short-hairpin RNAs against vesicular stomatitis virus ribonuclear complex genes.

# publications[].label
Efficient correction of a deleterious point mutation in primary horse fibroblasts with CRISPR-Cas9.

# publications[].label
In vitro production of porcine embryos from semen sorted for sex with a high speed cell sorter: Comparison of two fertilization media

# publications[].label
Cryopreservation of in vitro produced bovine embryos: effects of lipid segregation and post-thaw laser assisted hatching.

# publications[].label
Inhibition of EHMT2 Induces a Robust Antiviral Response Against Foot-and-Mouth Disease and Vesicular Stomatitis Virus Infections in Bovine Cells.

# publications[].label
Reshaping the transcriptional frontier: epigenetics and somatic cell nuclear transfer.

# publications[].label
Evaluation of culture systems for attachment and proliferation of epithelial cells cultured from ovine semen.

# publications[].label
Synchronisation of canine germinal vesicle stage oocytes prior to in vitro maturation alters the kinetics of nuclear progression during subsequent resumption of meiosis.

# publications[].label
Oxygen-induced alterations in the expression of chromatin modifying enzymes and the transcriptional regulation of imprinted genes.

# publications[].label
Harnessing a viral protein for efficient homologous recombination during mammalian genome editing

# publications[].label
In vitro production of sexed embryos for gender preselection: high-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer.

# publications[].label
Expression of porcine fusion protein IRF7/3(5D) efficiently controls foot-and-mouth disease virus replication.

# publications[].label
Lentivirus mediated transgenic livestock: applications in biomedical research

# publications[].label
A single injection of long-acting human FSH-N4 increases oocyte retrieval compared to follistim during ovarian hyperstimulation in the domestic goat (Capra hircus).

# publications[].label
Nuclear transfer in the bovine embryo: The effect of DC voltage and pulse duration on electrofusion and cell lysis

# publications[].label
Chromatin and microtubule morphology during the first cell cycle in bovine zygotes.

# publications[].label
337 PRODUCTION OF TRANSGENIC SHEEP USING RECOMBINANT LENTIVIRUS MICROINJECTION OF IN VIVO PRODUCED EMBRYOS

# publications[].label
Transgenic livestock for agriculture and biomedical applications

# publications[].label
86 IMPROVED BOVINE EMBRYO PRODUCTION USING NOVEL IN VITRO CULTURE SYSTEMS

# publications[].label
Adding and subtracting livestock genes with transposons and nucleases

# publications[].label
Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells.

# publications[].label
Maternal nutrient restriction in late pregnancy programs postnatal metabolism and pituitary development in beef heifers.

# publications[].label
Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification.

# publications[].label
Applications of RNA interference-based gene silencing in animal agriculture.

# publications[].label
145 REESTABLISHMENT OF AN EXTINCT STRAIN OF SHEEP UTILIZING ASSISTED REPRODUCTIVE TECHNOLOGIES

# publications[].label
Evaluation of culture and electroporation of feline fetal fibroblasts used to assess the effect of zinc finger nucleases on gene targeting efficiency

# publications[].label
In vitro production of pig embryos: comparisons of culture media and boars.

# publications[].label
INDUCTION OF MEIOTIC ARREST IN IMMATURE FELINE OOCYTES WITH ROSCOVITINE AND DIBUTYRYL CYCLIC-AMP

# publications[].label
Genetic correlations among reproductive traits and uterine dimensions in mice.

# publications[].label
Viral particles of endogenous betaretroviruses are released in the sheep uterus and infect the conceptus trophectoderm in a transspecies embryo transfer model.

# publications[].label
Identification, amplification and characterization of miR-17-92 from canine tissue.

# publications[].label
Evidence for Transmission of Endogenous Betaretroviruses from the Maternal Uterus to the Conceptus in Sheep.

# publications[].label
The Lentiviral System Construction for Highly Expressed Porcine Stearoyl-CoA Desaturase-1 and Functional Characterization in Stably Transduced Porcine Swine Kidney Cells.

# publications[].label
PSVII-5 Nutritional programming of prenatal beef heifer development and postnatal performance.

# publications[].label
Assessment of stallion sperm membrane integrity using three different flow cytometric methods

# publications[].label
DEVELOPMENT OF TRANSGENIC LIVESTOCK WITH REDUCED MYOSTATIN EXPRESSION USING RNA INTERFERENCE

# publications[].label
Histone-lysine N-methyltransferase SETDB1 is required for development of the bovine blastocyst.

# publications[].label
New commercial opportunities for advanced reproductive technologies in horses, wildlife, and companion animals.

# publications[].label
Correlation between histochemically assessed fiber type distribution and isomyosin and myosin heavy chain content in porcine skeletal muscles.

# publications[].label
Depletion of elongation initiation factor 4E binding proteins by CRISPR/Cas9 enhances the antiviral response in porcine cells.

# publications[].label
122 SUPPRESSION OF EPIGENETIC MODIFIERS ALTERS THE BOVINE EMBRYONIC DEVELOPMENTAL PROGRAM DURING IN VITRO CULTURE

# publications[].label
Verification of flow cytometorically-sorted X- and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique.

# publications[].label
Use of prepuberal heifers as oocyte donors for IVF: Effect of age and gonadotrophin treatment

# publications[].label
Transfection of bovine oocytes and preimplantation embryos with commercial transfection reagents: Comparison of embryonic stage and zona integrity on transfection efficiency.

# publications[].label
Transgenic sheep generated by lentiviral vectors: safety and integration analysis of surrogates and their offspring.

# publications[].label
Cytological characterization of maturation and fertilization in prepubertal calf oocytes

# publications[].label
Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos taurus hybrid fetuses.

# publications[].label
95 PRODUCTION OF GENE-EDITED PIGS, CATTLE, AND LAMBS BY EMBRYO INJECTION OF TALENS OR ZFNs

# publications[].label
THE EFFECT OF FORSKOLIN ON IN VITRO PRODUCED BRAHMAN-SIRED BOVINE EMBRYOS

# publications[].label
Expression profiling of histone-modifying genes in bovine preimplantation embryos

# publications[].label
Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development.

# publications[].label
140 UTILIZATION OF LASER-ASSISTED HATCHING TO IMPROVE THE PREGNANCY RATE OF IN VITRO -FERTILIZED BOVINE EMBRYOS

# publications[].label
Cloning of food animals and endangered species.

# publications[].label
Cryopreservation of swine embryos with reduced lipid content

# publications[].label
124 Cell Count of Bovine In Vitro-Produced Blastocysts After In Vitro Maturation in Glass versus Plastic Vials

# publications[].label
Down-regulation of viral replication by lentiviral-mediated expression of short-hairpin RNAs against vesicular stomatitis virus ribonuclear complex genes

# publications[].label
TRANSCRIPTIONAL PROFILING OF HISTONE-MODIFYING GENES DURING BOVINE PRE-IMPLANTATION EMBRYO DEVELOPMENT IN VITRO

# publications[].label
The effect of lipid segregation with or without zona pellucida laser drilling on post-thaw embryo development of in vitro-produced bovine embryos

# publications[].label
Evaluation of developmental competence, nuclear and ooplasmic maturation of calf oocytes.

# publications[].label
195 SUPPRESSION OF SETDB1 DURING BOVINE EMBRYONIC DEVELOPMENT RESULTS IN PRE-IMPLANTATION MORTALITY AND DECREASED TRANSCRIPTION OF TRANSPOSABLE ELEMENTS

# publications[].label
Efficient TALEN-mediated gene knockout in livestock.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Effect of sperm removal time, sperm concentration and motility enhancers on fertilization parameters and development of bovine embryos in vitro

# publications[].label
Effects of unilateral ovariectomy on reproductive traits of mice

# publications[].label
Re-Establishment and Characterization of an Extinct Line of Sheep with a Spontaneous Bleeding Disorder That Closely Recapitulates Human Hemophilia A.

# publications[].label
207 EFFICIENT GENERATION OF MYOSTATIN PROMOTER MUTATIONS IN BOVINE EMBRYOS USING THE CRISPR/Cas9 SYSTEM

# publications[].label
Manipulation of early bovine embryos: Effect on subsequent development and embryo quality in vitro

# researcherOn[].label
2014 BFP, TEXAS A&M, Animal AI #5, MOROCCO

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Effect of Prenatal Stress on DNA Methylation and Correspondence with Gene Expression in Cattle

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Modeling Odontohypophosphatasia in the Sheep

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Dental and Craniofacial Research

# researcherOn[].label
Role of miR15a and miR34c in PGE2 Signaling in the Pathogenesis of Endometriosis

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-Eunice Kennedy Shriver National Institute of Child Health & Human Development

# researcherOn[].label
Reproductive Performance in Domestic Ruminants

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Novel anti-TNF-alpha therapy to improve Equine perforamce and quality of life

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Inducible Tissue-Specific Transgene Expression In Large Animal Biomedical Models

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-Office of Director-Office of Research Infrastructure Programs

# researcherOn[].label
Genetic engineering of livestock to improve production of agricultural and biomedical products.

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Mechanism of Prenatal Chromium-VI Exposure and Germ Cell Apoptosis in the Ovary

# researcherOn[].awardedBy.label
National Institute of Environmental Health Sciences - (Durham, North Carolina, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Environmental Health Sciences

# streetAddress
Texas A&M University; Vet Med - Physiology & Pharmacology; 4466 TAMU

# organizations[]
College of Veterinary Medicine and Biomedical Sciences

# organizations[]
"College of Veterinary Medicine and Biomedical Sciences"@en-US

# schools[]
College of Veterinary Medicine and Biomedical Sciences

# schools[]
"College of Veterinary Medicine and Biomedical Sciences"@en-US

# etdChairOf[].label
Ramirez Carvajal, Lisbeth (2014-08). Enhancement of Innate Immunity to Control Viral Infections in Livestock Species. Doctoral Dissertation.

# etdChairOf[].label
Williamson, Gayle Linger (2011-08). Characterization and Analysis of the Bovine Epigenome during Preimplantation Embryo Development In Vitro. Master's Thesis.

# etdChairOf[].label
Veazey, Kylee Jordan (2015-12). An Epigenetic Basis to the Etiology of Fetal Alcohol Spectrum Disorders. Doctoral Dissertation.

# etdChairOf[].label
Smith, Melanie Love (2011-05). Blood Chemistry of Free-Ranging and Captive White-tailed Deer (Odocoileus virginianus) in Texas. Master's Thesis.

# etdChairOf[].label
Burns, Gregory Willis (2013-12). Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15. Master's Thesis.

# etdChairOf[].label
Stroud, Todd (2009-05). Reprogramming DNA Methylation in Bovine Cells by Knocking Down DNA Methyltransferase-1 with RNA Interference. Master's Thesis.

# etdChairOf[].label
Snyder, Matthew D (2016-08). Analysis of the Histone Methyltransferase ASH2L  via RNA Interference and CRISPR-Cas9  during Bovine Early Embryonic Development. Master's Thesis.

# etdChairOf[].label
Ramirez Carvajal, Lisbeth (2011-08). RNA Interference-Based Approach to Combat Viral Infections: Vesicular Stomatitis Virus Group Prototype. Master's Thesis.

# etdChairOf[].label
Georges, Hanah Michale (2017-08). Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR. Master's Thesis.

# etdChairOf[].label
Skiles, William Mark (2017-12). Environmentally Induced Epimutations, Their Persistence, and Potential Causality in the Development of Disease in the Offspring of Exposed Individuals. Doctoral Dissertation.