# positions[].organizations[].label
Texas A&M University Naresh K. Vashisht College of Medicine

# overview
My laboratory is focused on understanding the initiation of new blood vessel growth. Endothelial cells remain dormant for decades, yet when the proper signals to promote new blood vessel growth are present, these cells change phenotype rapidly to initiate the process.  Our group is interested in understanding the basic signals that control these events to ultimately inform clinical therapies to promote or inhibit new blood vessel growth, as needed. Our insights also inform whether or not biomaterials effectively promote new blood vessel growth in animal models.

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Receptor, Transforming Growth Factor-beta Type I

# keywords[]
Receptors, Transforming Growth Factor Beta

# keywords[]
Stem Cell Research - Nonembryonic - Human

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
4012 Fluid Mechanics And Thermal Engineering

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Receptor, Transforming Growth Factor-beta Type I

# keywords[]
Receptors, Transforming Growth Factor Beta

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
Hypoxia-Inducible Factor 1, Alpha Subunit

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
Human Uterine Microvascular Endothelial Cells

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Perinatal Period - Conditions Originating In Perinatal Period

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
Receptor, Transforming Growth Factor-beta Type I

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
Vascular Endothelial Growth Factor Receptor-3

# keywords[]
Perinatal Period - Conditions Originating In Perinatal Period

# keywords[]
Vascular Endothelial Growth Factor Receptor-3

# keywords[]
Vascular Endothelial Growth Factor Receptor-2

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# educationAndTraining[].organization.label
Texas A&M Health Science Center - (College Station, Texas, United States)

# educationAndTraining[].organization.label
Texas Lutheran University - (Seguin, Texas, United States)

# educationAndTraining[].organization.label
Texas A&M Health Science Center - (College Station, Texas, United States)

# awardsAndHonors[].label
Junior Faculty Research Excellence Award

# publications[].label
Nonlinear optical microscopy reveals invading endothelial cells anisotropically alter three-dimensional collagen matrices.

# publications[].label
SPP1 (Osteopontin) and SPARC (Osteonectin) May Interact Developmentally During Mouse Pregnancy.

# publications[].label
Selective regulation of Notch ligands during angiogenesis is mediated by vimentin.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Angiogenesis: 2D Nanosilicates Loaded with Proangiogenic Factors Stimulate Endothelial Sprouting (Adv. Biosys. 7/2018)

# publications[].label
Constitutive activation of transforming growth factor Beta receptor 1 in the mouse uterus impairs uterine morphology and function.

# publications[].label
Vimentin as an integral regulator of cell adhesion and endothelial sprouting.

# publications[].label
Integrin  is necessary for the attachment of trophectoderm cells to osteopontin to mediate adhesion of the conceptus to the uterus during implantation in pigs

# publications[].label
RGD-dependent vacuolation and lumen formation observed during endothelial cell morphogenesis in three-dimensional fibrin matrices involves the alpha(v)beta(3) and alpha(5)beta(1) integrins.

# publications[].label
Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

# publications[].label
Isolation and biological properties of osteopontin from bovine milk.

# publications[].label
Recruitment and Modulation of Porcine Endothelial Progenitor Cells by Secreted Phosphoprotein 1 (SPP1, Osteopontin).

# publications[].label
Microtubule depolymerization rapidly collapses capillary tube networks in vitro and angiogenic vessels in vivo through the small GTPase Rho.

# publications[].label
Annexin 2 regulates endothelial morphogenesis by controlling AKT activation and junctional integrity.

# publications[].label
Separating full-length protein from aggregating proteolytic products using filter flow-through purification.

# publications[].label
Osteopontin is a ligand for the alpha4beta1 integrin.

# publications[].label
The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices

# publications[].label
Conjugated Bile Acids activate Reactive Oxygen Speciesp90RSKVascular Endothelial Growth Factor Receptor 3 signaling axis to promote lymphangiogenesis

# publications[].label
Effects of Fluid Shear Stress on Endothelial Cell Invasion Into Three-Dimensional Matrices

# publications[].label
Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP.

# publications[].label
Identification of New Markers of Angiogenic Sprouting Using Transcriptomics: New Role for RND3.

# publications[].publisher
Ovid Technologies (Wolters Kluwer Health)

# publications[].label
The sphingosine 1-phosphate (S1P) signaling pathway is regulated during pregnancy in sheep.

# publications[].label
MMP-1 activation by serine proteases and MMP-10 induces human capillary tubular network collapse and regression in 3D collagen matrices.

# publications[].label
AFFINITY OF OSTEOPONTIN FOR LEUKOCYTE INTEGRINS, EXTRACELLULAR MATRICES AND APOPTOTIC CELLS

# publications[].label
The NADPH oxidase 2 subunit p47phox binds to the WAVE regulatory complex and p22phox in a mutually exclusive manner.

# publications[].label
Molecular Regulation of Sprouting Angiogenesis.

# publications[].label
Identification of new markers of angiogenic sprouting using transcriptomics: New role for RND3

# publications[].label
Fluid shear stress modulates endothelial cell invasion into three-dimensional collagen matrices.

# publications[].label
Mechanotransduction drives morphogenesis to develop folding during placental development in pigs.

# publications[].label
ADAM17 co-purifies with TIMP-3 and modulates endothelial invasion responses in three-dimensional collagen matrices.

# publications[].label
Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal-conceptus interface and uterine wall during ovine pregnancy.

# publications[].label
TWIST1 and TSG6 are coordinately regulated and function as potency biomarkers in human MSCs.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Fluid shear stress and sphingosine 1-phosphate promote Filamin A membrane translocation and complex formation with VE-cadherin during endothelial cell invasion.

# publications[].label
Secreted Phosphoprotein 1 (SPP1, Osteopontin) Stimulates Adhesion and Migration of Circulating Endothelial Cells in Pigs.

# publications[].label
Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Is Required for Uterine Epithelial Integrity.

# publications[].label
ITGAV (alpha v integrins) bind SPP1 (osteopontin) to support trophoblast cell adhesion.

# publications[].label
Regulation of tissue injury responses by the exposure of matricryptic sites within extracellular matrix molecules.

# publications[].label
Nanoengineered injectable hydrogels for wound healing application.

# publications[].label
Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.

# publications[].label
An integrin and Rho GTPase-dependent pinocytic vacuole mechanism controls capillary lumen formation in collagen and fibrin matrices.

# publications[].label
Synergistic Regulation of Angiogenic Sprouting by Biochemical Factors and Wall Shear Stress.

# publications[].label
Endorepellin and endostatin affect the neurovascular unit in vitro

# publications[].label
CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and function.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing.

# publications[].label
Osteopontin is a ligand for the alpha4beta1 integrin.

# publications[].label
Identification of multiple dityrosine bonds in materials composed of the Drosophila protein Ultrabithorax.

# publications[].label
Expression of SPP1 (Osteopontin) and the Aggregation of Integrins into Focal Adhesions Is an Adaptation to Mechanical Forces at the Uterine-Placental Interface in Pigs.

# publications[].label
Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation.

# publications[].label
TGFBR1 is required for mouse myometrial development.

# publications[].label
Fluid Shear Stress Modulates S1P-Induced Endothelial Cell Invasion Into Three-Dimensional Matrices

# publications[].label
Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation

# publications[].label
Ethanol impairs Rho GTPase signaling and differentiation of cerebellar granule neurons in a rodent model of fetal alcohol syndrome.

# publications[].label
Investigating endothelial invasion and sprouting behavior in three-dimensional collagen matrices.

# publications[].label
Functionalized protein-based materials and their uses

# publications[].label
Guanosine triphosphatase activation occurs downstream of calcineurin in cardiac hypertrophy*.

# publications[].label
Direct Involvement of CD8+ T Cells in Retinal Angiogenesis.

# publications[].label
Engineering ubx-based materials to promote and guide neovascularization

# publications[].label
OPN binds alpha V integrin to promote endothelial progenitor cell incorporation into vasculature.

# publications[].label
The NF-B RelB protein is an oncogenic driver of mesenchymal glioma.

# publications[].label
Abstract W MP51: Insulin-like Growth Factor (igf-1) Enhances The Angiogenic Response Of Aging Endothelial Cells

# publications[].label
MicroRNA-150 (miR-150) and Diabetic Retinopathy: Is miR-150 Only a Biomarker or Does It Contribute to Disease Progression?

# publications[].label
ULTRASHORT PULSE MULTISPECTRAL NONLINEAR OPTICAL MICROSCOPY.

# publications[].label
Dynamic multicomponent engineered tissue reorganization and matrix deposition measured with an integrated nonlinear optical microscopy-optical coherence microscopy system.

# publications[].publisher
SPIE, the international society for optics and photonics

# publications[].label
S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses.

# publications[].label
Calpain-mediated vimentin cleavage occurs upstream of MT1-MMP membrane translocation to facilitate endothelial sprout initiation.

# publications[].label
Hypoxic tumor microenvironment: Implications for cancer therapy.

# publications[].label
Akt, 14-3-3, and vimentin mediate a drug-resistant invasive phenotype in diffuse large B-cell lymphoma.

# publications[].label
Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase.

# publications[].label
Fluid shear stress and sphingosine 1-phosphate activate calpain to promote membrane type 1 matrix metalloproteinase (MT1-MMP) membrane translocation and endothelial invasion into three-dimensional collagen matrices.

# publications[].label
Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration.

# publications[].label
Establishment of a three-dimensional model to study human uterine angiogenesis.

# publications[].label
Fiber Optically Delivered Laser Pulses for Multispectral Nonlinear Optical Microscopy

# publications[].label
Rho plays a central role in regulating local cell-matrix mechanical interactions in 3D culture.

# publications[].label
Conceptus-uterus interactions in pigs: endometrial gene expression in response to estrogens and interferons from conceptuses

# publications[].label
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.

# publications[].label
Assembly of a mechanosensitive complex is required to initiate sprouting angiogenesis

# publications[].label
2D Nanosilicates Loaded with Proangiogenic Factors Stimulate Endothelial Sprouting

# publications[].label
Cyclin A1 is a p53-induced gene that mediates apoptosis, G2/M arrest, and mitotic catastrophe in renal, ovarian, and lung carcinoma cells.

# publications[].label
Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling.

# publications[].label
Antagonism of the Sphingosine 1-Phosphate (S1P) Signaling Pathway in Pregnant Ewes Alters Placentome Architecture, Amino Acid Transport, and Fetal Growth.

# publications[].label
Capillary morphogenesis during human endothelial cell invasion of three-dimensional collagen matrices

# publications[].label
Molecular basis of endothelial cell morphogenesis in three-dimensional extracellular matrices.

# publications[].label
Novel pathways for implantation and establishment and maintenance of pregnancy in mammals.

# publications[].label
Genetic Functionalization of Protein-Based Biomaterials via Protein Fusions.

# publications[].label
EZH2 and Endometrial Cancer Development: Insights from a Mouse Model.

# publications[].label
Sphingosine-1-phosphate markedly induces matrix metalloproteinase and integrin-dependent human endothelial cell invasion and lumen formation in three-dimensional collagen and fibrin matrices.

# publications[].label
Functionalization of Ultrabithorax Materials with Vascular Endothelial Growth Factor Enhances Angiogenic Activity.

# publications[].label
Transforming growth factor beta signaling and decidual integrity in mice.

# publications[].label
Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network.

# publications[].label
Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress.

# publications[].label
Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating native capillary network

# publications[].label
Conceptus-uterus interactions in pigs: endometrial gene expression in response to estrogens and interferons from conceptuses.

# publications[].label
The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices.

# publications[].label
Proteomic profiling of endothelial invasion revealed receptor for activated C kinase 1 (RACK1) complexed with vimentin to regulate focal adhesion kinase (FAK).

# publications[].label
Tumor Lymphatic Interactions Induce CXCR2-CXCL5 Axis and Alter Cellular Metabolism and Lymphangiogenic Pathways to Promote Cholangiocarcinoma.

# publications[].label
Capillary morphogenesis during human endothelial cell invasion of three-dimensional collagen matrices.

# publications[].label
Endothelial tubes assemble from intracellular vacuoles in vivo.

# publications[].label
Ischemia-induced cleavage of cadherins in NRK cells: evidence for a role of metalloproteinases.

# publications[].label
Effects of Fluid Shear Stress on Endothelial Cell Invasion Into Three-Dimensional Matrices

# publications[].label
Fluid shear stress enhances the effects of sphingosine-1-phosphate on endothelial cell invasion into collagen matrices

# publications[].label
Identification of dual alpha 4beta1 integrin binding sites within a 38 amino acid domain in the N-terminal thrombin fragment of human osteopontin.

# publications[].label
Targeting Lymphangiogenesis and Lymph Node Metastasis in Liver Cancer.

# publications[].label
NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-B pathway.

# publications[].label
Effects of fluid shear stress on endothelial cell invasion into three-dimensional matrices

# publications[].label
FTY720, a sphingosine analog, altered placentome histoarchitecture in ewes.

# publications[].label
Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cells.

# publications[].label
Hypertensive Stimuli Indirectly Stimulate Lymphangiogenesis through Immune Cell Secreted Factors.

# publications[].label
Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness.

# publications[].label
Progesterone and placentation increase secreted phosphoprotein one (SPP1 or osteopontin) in uterine glands and stroma for histotrophic and hematotrophic support of ovine pregnancy.

# publications[].label
MATRICRYPTIC INTEGRIN BINDING SITES IN OSTEOPONTIN REGULATE INFLAMMATION

# publications[].label
Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species-p90RSK-Vascular Endothelial Growth Factor Receptor 3 Pathway.

# publications[].label
Materials composed of the Drosophila melanogaster protein ultrabithorax are cytocompatible.

# publications[].label
Matrix density alters zyxin phosphorylation, which limits peripheral process formation and extension in endothelial cells invading 3D collagen matrices.

# publications[].label
Role of the cytoskeleton in formation and maintenance of angiogenic sprouts.

# publications[].label
Antagonism of the S1P Signaling Pathway Affects Placental and Fetal Development.

# publications[].label
The 1-integrin plays a key role in LEC invasion in an optimized 3-D collagen matrix model.

# publications[].label
Nanosilicates promote angiogenesis through activation of ROS-mediated WNT/-catenin pathway.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Newly Identified Peptide, Peptide Lv, Promotes Pathological Angiogenesis.

# researcherOn[].label
Mechanisms of Angiogenic Switch Activation During Wound Repair

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Heart, Lung, and Blood Institute

# researcherOn[].label
Filamins stabilize a transmembrane complex to initiate angiogenesis

# researcherOn[].awardedBy.label
American Heart Association - (New York, New York, United States)

# researcherOn[].label
NIK Promotes a Leader Cell Phenotype in Glioma

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Neurological Disorders and Stroke

# streetAddress
Texas A&M Health Science Center; Medical Physiology; 8447 Medical Research Building 2

# organizations[]
Texas A&M University Naresh K. Vashisht College of Medicine

# etdChairOf[].label
Smith, Rebecca (2014-05). Annexin A2 is Required for Endothelial Cell Junctional Response to S1P. Master's Thesis.

# etdChairOf[].label
Tsai, Shang-Pu (2016-05). Ubx as a Novel Protein-Based Material: Structural Insights, Functionalization via Gene Fusion and Biomedical Applications. Doctoral Dissertation.

# etdChairOf[].label
Su, Shih-Chi (2011-05). Study of Endothelial Morphogenesis in Three-Dimensional Collagen Matrices. Doctoral Dissertation.

# etdChairOf[].label
Duran, Camille L (2017-05). Noncanonical NF-KB Signaling Drives Glioma Invasion by Promoting MT1-MMP Activation, Pseudopodia Formation, and ITGA11 Expression. Doctoral Dissertation.

# etdChairOf[].label
Kang, Ho Jin (2011-05). The Synergic Effects of Flow and Sphingosine 1-Phosphate on Sprouting Angiogenesis Into Three-Dimensional Collagen Matrices. Doctoral Dissertation.

# etdChairOf[].label
Dave, Jui Mayank (2014-12). Identification of Scaffold Proteins RACK1 and Hic-5 as Regulators of Endothelial Sprouting Resoponses. Doctoral Dissertation.