# positions[].organizations[].label
Texas A&M University Naresh K. Vashisht College of Medicine

# overview
My laboratory is focused on understanding the initiation of new blood vessel growth. Endothelial cells remain dormant for decades, yet when the proper signals to promote new blood vessel growth are present, these cells change phenotype rapidly to initiate the process.  Our group is interested in understanding the basic signals that control these events to ultimately inform clinical therapies to promote or inhibit new blood vessel growth, as needed. Our insights also inform whether or not biomaterials effectively promote new blood vessel growth in animal models.

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Receptors, Transforming Growth Factor Beta

# keywords[]
Receptor, Transforming Growth Factor-beta Type I

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Hypoxia-Inducible Factor 1, Alpha Subunit

# keywords[]
Receptors, Transforming Growth Factor Beta

# keywords[]
Receptor, Transforming Growth Factor-beta Type I

# keywords[]
Human Uterine Microvascular Endothelial Cells

# keywords[]
Stem Cell Research - Nonembryonic - Human

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
4012 Fluid Mechanics And Thermal Engineering

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
Vascular Endothelial Growth Factor Receptor-3

# keywords[]
Perinatal Period - Conditions Originating In Perinatal Period

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Receptor, Transforming Growth Factor-beta Type I

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Perinatal Period - Conditions Originating In Perinatal Period

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Vascular Endothelial Growth Factor Receptor-3

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Vascular Endothelial Growth Factor Receptor-2

# educationAndTraining[].organization.label
Texas A&M Health Science Center - (College Station, Texas, United States)

# educationAndTraining[].organization.label
Texas Lutheran University - (Seguin, Texas, United States)

# educationAndTraining[].organization.label
Texas A&M Health Science Center - (College Station, Texas, United States)

# awardsAndHonors[].label
Junior Faculty Research Excellence Award

# publications[].label
Integrin  is necessary for the attachment of trophectoderm cells to osteopontin to mediate adhesion of the conceptus to the uterus during implantation in pigs

# publications[].label
Annexin 2 regulates endothelial morphogenesis by controlling AKT activation and junctional integrity.

# publications[].label
2D Nanosilicates Loaded with Proangiogenic Factors Stimulate Endothelial Sprouting

# publications[].label
Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating native capillary network

# publications[].label
Molecular basis of endothelial cell morphogenesis in three-dimensional extracellular matrices.

# publications[].label
Proteomic profiling of endothelial invasion revealed receptor for activated C kinase 1 (RACK1) complexed with vimentin to regulate focal adhesion kinase (FAK).

# publications[].label
Rho plays a central role in regulating local cell-matrix mechanical interactions in 3D culture.

# publications[].label
The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices.

# publications[].label
The sphingosine 1-phosphate (S1P) signaling pathway is regulated during pregnancy in sheep.

# publications[].label
Investigating endothelial invasion and sprouting behavior in three-dimensional collagen matrices.

# publications[].label
Endothelial tubes assemble from intracellular vacuoles in vivo.

# publications[].label
Identification of new markers of angiogenic sprouting using transcriptomics: New role for RND3

# publications[].label
AFFINITY OF OSTEOPONTIN FOR LEUKOCYTE INTEGRINS, EXTRACELLULAR MATRICES AND APOPTOTIC CELLS

# publications[].label
Osteopontin is a ligand for the alpha4beta1 integrin.

# publications[].label
TGFBR1 is required for mouse myometrial development.

# publications[].label
Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cells.

# publications[].label
Identification of New Markers of Angiogenic Sprouting Using Transcriptomics: New Role for RND3.

# publications[].publisher
Ovid Technologies (Wolters Kluwer Health)

# publications[].label
Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing.

# publications[].label
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.

# publications[].label
Abstract W MP51: Insulin-like Growth Factor (igf-1) Enhances The Angiogenic Response Of Aging Endothelial Cells

# publications[].label
Secreted Phosphoprotein 1 (SPP1, Osteopontin) Stimulates Adhesion and Migration of Circulating Endothelial Cells in Pigs.

# publications[].label
RGD-dependent vacuolation and lumen formation observed during endothelial cell morphogenesis in three-dimensional fibrin matrices involves the alpha(v)beta(3) and alpha(5)beta(1) integrins.

# publications[].label
ULTRASHORT PULSE MULTISPECTRAL NONLINEAR OPTICAL MICROSCOPY.

# publications[].label
TWIST1 and TSG6 are coordinately regulated and function as potency biomarkers in human MSCs.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Fluid shear stress modulates endothelial cell invasion into three-dimensional collagen matrices.

# publications[].label
An integrin and Rho GTPase-dependent pinocytic vacuole mechanism controls capillary lumen formation in collagen and fibrin matrices.

# publications[].label
Hypoxic tumor microenvironment: Implications for cancer therapy.

# publications[].label
ITGAV (alpha v integrins) bind SPP1 (osteopontin) to support trophoblast cell adhesion.

# publications[].label
Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP.

# publications[].label
Selective regulation of Notch ligands during angiogenesis is mediated by vimentin.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Materials composed of the Drosophila melanogaster protein ultrabithorax are cytocompatible.

# publications[].label
Nanoengineered injectable hydrogels for wound healing application.

# publications[].label
Role of the cytoskeleton in formation and maintenance of angiogenic sprouts.

# publications[].label
Sphingosine-1-phosphate markedly induces matrix metalloproteinase and integrin-dependent human endothelial cell invasion and lumen formation in three-dimensional collagen and fibrin matrices.

# publications[].label
Assembly of a mechanosensitive complex is required to initiate sprouting angiogenesis

# publications[].label
Tumor Lymphatic Interactions Induce CXCR2-CXCL5 Axis and Alter Cellular Metabolism and Lymphangiogenic Pathways to Promote Cholangiocarcinoma.

# publications[].label
Matrix density alters zyxin phosphorylation, which limits peripheral process formation and extension in endothelial cells invading 3D collagen matrices.

# publications[].label
Targeting Lymphangiogenesis and Lymph Node Metastasis in Liver Cancer.

# publications[].label
Constitutive activation of transforming growth factor Beta receptor 1 in the mouse uterus impairs uterine morphology and function.

# publications[].label
Fiber Optically Delivered Laser Pulses for Multispectral Nonlinear Optical Microscopy

# publications[].label
Osteopontin is a ligand for the alpha4beta1 integrin.

# publications[].label
Calpain-mediated vimentin cleavage occurs upstream of MT1-MMP membrane translocation to facilitate endothelial sprout initiation.

# publications[].label
Genetic Functionalization of Protein-Based Biomaterials via Protein Fusions.

# publications[].label
Engineering ubx-based materials to promote and guide neovascularization

# publications[].label
MMP-1 activation by serine proteases and MMP-10 induces human capillary tubular network collapse and regression in 3D collagen matrices.

# publications[].label
Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal-conceptus interface and uterine wall during ovine pregnancy.

# publications[].label
Functionalization of Ultrabithorax Materials with Vascular Endothelial Growth Factor Enhances Angiogenic Activity.

# publications[].label
Establishment of a three-dimensional model to study human uterine angiogenesis.

# publications[].label
Microtubule depolymerization rapidly collapses capillary tube networks in vitro and angiogenic vessels in vivo through the small GTPase Rho.

# publications[].label
Cyclin A1 is a p53-induced gene that mediates apoptosis, G2/M arrest, and mitotic catastrophe in renal, ovarian, and lung carcinoma cells.

# publications[].label
Angiogenesis: 2D Nanosilicates Loaded with Proangiogenic Factors Stimulate Endothelial Sprouting (Adv. Biosys. 7/2018)

# publications[].label
Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress.

# publications[].label
Recruitment and Modulation of Porcine Endothelial Progenitor Cells by Secreted Phosphoprotein 1 (SPP1, Osteopontin).

# publications[].label
Capillary morphogenesis during human endothelial cell invasion of three-dimensional collagen matrices.

# publications[].label
Isolation and biological properties of osteopontin from bovine milk.

# publications[].label
CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and function.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
OPN binds alpha V integrin to promote endothelial progenitor cell incorporation into vasculature.

# publications[].label
Guanosine triphosphatase activation occurs downstream of calcineurin in cardiac hypertrophy*.

# publications[].label
The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices

# publications[].label
Conceptus-uterus interactions in pigs: endometrial gene expression in response to estrogens and interferons from conceptuses.

# publications[].label
Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation.

# publications[].label
S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses.

# publications[].label
The NADPH oxidase 2 subunit p47phox binds to the WAVE regulatory complex and p22phox in a mutually exclusive manner.

# publications[].label
Endorepellin and endostatin affect the neurovascular unit in vitro

# publications[].label
Novel pathways for implantation and establishment and maintenance of pregnancy in mammals.

# publications[].label
NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-B pathway.

# publications[].label
Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness.

# publications[].label
Functionalized protein-based materials and their uses

# publications[].label
Effects of Fluid Shear Stress on Endothelial Cell Invasion Into Three-Dimensional Matrices

# publications[].label
Nanosilicates promote angiogenesis through activation of ROS-mediated WNT/-catenin pathway.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Ethanol impairs Rho GTPase signaling and differentiation of cerebellar granule neurons in a rodent model of fetal alcohol syndrome.

# publications[].label
Fluid shear stress enhances the effects of sphingosine-1-phosphate on endothelial cell invasion into collagen matrices

# publications[].label
EZH2 and Endometrial Cancer Development: Insights from a Mouse Model.

# publications[].label
SPP1 (Osteopontin) and SPARC (Osteonectin) May Interact Developmentally During Mouse Pregnancy.

# publications[].label
Ischemia-induced cleavage of cadherins in NRK cells: evidence for a role of metalloproteinases.

# publications[].label
Akt, 14-3-3, and vimentin mediate a drug-resistant invasive phenotype in diffuse large B-cell lymphoma.

# publications[].label
Progesterone and placentation increase secreted phosphoprotein one (SPP1 or osteopontin) in uterine glands and stroma for histotrophic and hematotrophic support of ovine pregnancy.

# publications[].label
Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling.

# publications[].label
Direct Involvement of CD8+ T Cells in Retinal Angiogenesis.

# publications[].label
Nonlinear optical microscopy reveals invading endothelial cells anisotropically alter three-dimensional collagen matrices.

# publications[].label
Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network.

# publications[].label
ADAM17 co-purifies with TIMP-3 and modulates endothelial invasion responses in three-dimensional collagen matrices.

# publications[].label
FTY720, a sphingosine analog, altered placentome histoarchitecture in ewes.

# publications[].label
Effects of fluid shear stress on endothelial cell invasion into three-dimensional matrices

# publications[].label
Hypertensive Stimuli Indirectly Stimulate Lymphangiogenesis through Immune Cell Secreted Factors.

# publications[].label
MATRICRYPTIC INTEGRIN BINDING SITES IN OSTEOPONTIN REGULATE INFLAMMATION

# publications[].label
The NF-B RelB protein is an oncogenic driver of mesenchymal glioma.

# publications[].label
Regulation of tissue injury responses by the exposure of matricryptic sites within extracellular matrix molecules.

# publications[].label
Fluid shear stress and sphingosine 1-phosphate activate calpain to promote membrane type 1 matrix metalloproteinase (MT1-MMP) membrane translocation and endothelial invasion into three-dimensional collagen matrices.

# publications[].label
Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration.

# publications[].label
Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

# publications[].label
Synergistic Regulation of Angiogenic Sprouting by Biochemical Factors and Wall Shear Stress.

# publications[].label
Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species-p90RSK-Vascular Endothelial Growth Factor Receptor 3 Pathway.

# publications[].label
MicroRNA-150 (miR-150) and Diabetic Retinopathy: Is miR-150 Only a Biomarker or Does It Contribute to Disease Progression?

# publications[].label
Antagonism of the S1P Signaling Pathway Affects Placental and Fetal Development.

# publications[].label
Capillary morphogenesis during human endothelial cell invasion of three-dimensional collagen matrices

# publications[].label
Conceptus-uterus interactions in pigs: endometrial gene expression in response to estrogens and interferons from conceptuses

# publications[].label
Identification of dual alpha 4beta1 integrin binding sites within a 38 amino acid domain in the N-terminal thrombin fragment of human osteopontin.

# publications[].label
Transforming growth factor beta signaling and decidual integrity in mice.

# publications[].label
Identification of multiple dityrosine bonds in materials composed of the Drosophila protein Ultrabithorax.

# publications[].label
Mechanotransduction drives morphogenesis to develop folding during placental development in pigs.

# publications[].label
Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Is Required for Uterine Epithelial Integrity.

# publications[].label
Expression of SPP1 (Osteopontin) and the Aggregation of Integrins into Focal Adhesions Is an Adaptation to Mechanical Forces at the Uterine-Placental Interface in Pigs.

# publications[].label
Vimentin as an integral regulator of cell adhesion and endothelial sprouting.

# publications[].label
Fluid shear stress and sphingosine 1-phosphate promote Filamin A membrane translocation and complex formation with VE-cadherin during endothelial cell invasion.

# publications[].label
Antagonism of the Sphingosine 1-Phosphate (S1P) Signaling Pathway in Pregnant Ewes Alters Placentome Architecture, Amino Acid Transport, and Fetal Growth.

# publications[].label
Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase.

# publications[].label
Conjugated Bile Acids activate Reactive Oxygen Speciesp90RSKVascular Endothelial Growth Factor Receptor 3 signaling axis to promote lymphangiogenesis

# publications[].label
Effects of Fluid Shear Stress on Endothelial Cell Invasion Into Three-Dimensional Matrices

# publications[].label
Separating full-length protein from aggregating proteolytic products using filter flow-through purification.

# publications[].label
Dynamic multicomponent engineered tissue reorganization and matrix deposition measured with an integrated nonlinear optical microscopy-optical coherence microscopy system.

# publications[].publisher
SPIE, the international society for optics and photonics

# publications[].label
Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.

# publications[].label
Molecular Regulation of Sprouting Angiogenesis.

# publications[].label
Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation

# publications[].label
Fluid Shear Stress Modulates S1P-Induced Endothelial Cell Invasion Into Three-Dimensional Matrices

# publications[].label
The 1-integrin plays a key role in LEC invasion in an optimized 3-D collagen matrix model.

# publications[].label
Newly Identified Peptide, Peptide Lv, Promotes Pathological Angiogenesis.

# researcherOn[].label
Mechanisms of Angiogenic Switch Activation During Wound Repair

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Heart, Lung, and Blood Institute

# researcherOn[].label
NIK Promotes a Leader Cell Phenotype in Glioma

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Neurological Disorders and Stroke

# researcherOn[].label
Filamins stabilize a transmembrane complex to initiate angiogenesis

# researcherOn[].awardedBy.label
American Heart Association - (New York, New York, United States)

# streetAddress
Texas A&M Health Science Center; Medical Physiology; 8447 Medical Research Building 2

# organizations[]
Texas A&M University Naresh K. Vashisht College of Medicine

# etdChairOf[].label
Tsai, Shang-Pu (2016-05). Ubx as a Novel Protein-Based Material: Structural Insights, Functionalization via Gene Fusion and Biomedical Applications. Doctoral Dissertation.

# etdChairOf[].label
Dave, Jui Mayank (2014-12). Identification of Scaffold Proteins RACK1 and Hic-5 as Regulators of Endothelial Sprouting Resoponses. Doctoral Dissertation.