# positions[].organizations[].parent[].label
Texas A&M University Naresh K. Vashisht College of Medicine

# overview
My laboratory studies the regulation of microvascular function at the level of arterioles in the retinal and coronary circulations. Sufficient blood flow supply of oxygen and nutrients to tissues to maintain normal function is controlled in large part by changes in the diameter of arterioles. Vasoconstriction or vasodilation of these small arteries will decrease or increase blood flow and nutrient delivery to the tissue, respectively. Two key chemical factors that are produced within the endothelial cells of blood vessels to control their diameter are nitric oxide (NO), a vasodilator, and endothelin-1, a vasoconstrictor. An imbalance in the production and/or release of these vasoactive factors has been implicated in the early stages of several cardiovascular diseases, but the underlying mechanisms contributing to these pathophysiological changes remain unclear. To address this knowledge gap, our research focuses on identifying cellular and molecular mechanisms that contribute to the vasomotor responses of arterioles to NO and endothelin-1 under conditions of health and disease. Current approaches that we use to investigate these mechanisms in the microcirculation include isolated and perfused arterioles, cultured vascular endothelial and smooth muscle cells, biochemical and molecular techniques (for detection of NO, superoxide anion, protein, and mRNA in arterioles), pharmacological and silencing RNA (siRNA) treatments, and blood flow velocity assessment via Doppler ultrasound.

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
Atp Binding Cassette Transporter, Subfamily B

# keywords[]
Vascular Endothelial Growth Factor Receptor-2

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Confocal Scanning Laser Ophthalmoscopy (cslo)

# keywords[]
Vascular Endothelial Growth Factor Receptor-2

# keywords[]
Vascular Endothelial Growth Factor Receptor-2

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Vascular Endothelial Growth Factor Receptor-2

# educationAndTraining[].organization.label
St. Olaf College - (Northfield, Minnesota, United States)

# educationAndTraining[].organization.label
Texas A&M University - (College Station, Texas, United States)

# educationAndTraining[].organization.label
Texas A&M University - (College Station, Texas, United States)

# awardsAndHonors[].label
Association of Former Students College-Level Teaching Distinguished Achievement Award, The

# awardsAndHonors[].label
August Krogh Oustanding Young Investigator Award

# publications[].label
Intraluminal pressure influences K-ATP channel-mediated coronary arteriolar dilation

# publications[].label
Role of endothelium in vasomotor responses to endothelin system and protein kinase C activation in porcine retinal arterioles.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Activation of JNK and xanthine oxidase by TNF-alpha impairs nitric oxide-mediated dilation of coronary arterioles.

# publications[].label
Simvastatin elicits dilation of isolated porcine retinal arterioles: role of nitric oxide and mevalonate-rho kinase pathways.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Hydrogen peroxide specifically impairs endothelium-dependent nitric oxide-mediated dilation of coronary arterioles: Role of arginase

# publications[].label
Stanniocalcin-1 enhances ellipsoid zone intensity and cone function in the P23H rhodopsin transgenic pig

# publications[].label
Role of Arginase in Selective Impairment of Endothelium-Dependent Nitric Oxide Synthase-Mediated Dilation of Retinal Arterioles during Early Diabetes.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Selective activation of lectin-like oxidized low-density lipoprotein receptor-1 mediates C-reactive protein-evoked endothelial vasodilator dysfunction in coronary arterioles.

# publications[].label
Divergent roles of nitric oxide and rho kinase in vasomotor regulation of human retinal arterioles.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Tumor necrosis factor-alpha-induced activation of JNK impairs nitric oxide-mediated dilation of coronary Arterioles

# publications[].label
CORONARY VASOMOTOR REGULATION BY ENDOTHELIN-1 AND OXIDATIVE STRESS

# publications[].label
Upregulation of vascular arginase in hypertension decreases nitric oxide-mediated dilation of coronary arterioles.

# publications[].label
Activation of barium-sensitive inward rectifier potassium channels mediates remote dilation of coronary arterioles.

# publications[].label
Requisite Roles of Endothelial Nitric Oxide Synthase and Smooth Muscle Phosphoinositide 3-Kinase/Rho Kinase In Vasomotor Regulation of Human Retinal Arterioles

# publications[].label
Recombinant interleukin-1 dilates steelhead trout coronary microvessels: effect of temperature and role of the endothelium, nitric oxide and prostaglandins.

# publications[].label
Contributions of Sodium-Hydrogen Exchanger 1 and Mitogen-Activated Protein Kinases to Enhanced Retinal Venular Constriction to Endothelin-1 in Diabetes.

# publications[].label
Integrin-binding peptides containing RGD produce coronary arteriolar dilation via cyclooxygenase activation.

# publications[].label
C-reactive protein inhibits endothelium-dependent nitric oxide-mediated dilation of retinal arterioles via enhanced superoxide production.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Constriction of Retinal Venules to Endothelin-1: Obligatory Roles of ETA Receptors, Extracellular Calcium Entry, and Rho Kinase.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Hyperglycemia Augments Endothelin-1-Induced Constriction of Human Retinal Venules.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Acute retinal ischemia inhibits endothelium-dependent nitric oxide-mediated dilation of retinal arterioles via enhanced superoxide production.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
C-reactive protein dilates coronary arterioles via barium-sensitive inward rectifier potassium channels

# publications[].label
Brimonidine evokes heterogeneous vasomotor response of retinal arterioles: diminished nitric oxide-mediated vasodilation when size goes small.

# publications[].label
Acute and Chronic Hyperglycemia Elicit JIP1/JNK-Mediated Endothelial Vasodilator Dysfunction of Retinal Arterioles.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Sodium azide dilates coronary arterioles via activation of inward rectifier K+ channels and Na+-K+-ATPase.

# publications[].label
Transmural difference in coronary arteriolar dilation to adenosine: effect of luminal pressure and K(ATP) channels.

# publications[].label
LDLs impair vasomotor function of the coronary microcirculation: role of superoxide anions.

# publications[].label
Coronary arteriolar dilation to K+: Role of smooth muscle Kir2.1 channels and Na+/K+ ATPase

# publications[].label
Histamine-Induced Dilation of Isolated Porcine Retinal Arterioles: Role of Endothelium-Derived Hyperpolarizing Factor.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
C-reactive protein impairs coronary arteriolar dilation to prostacyclin synthase activation: role of peroxynitrite.

# publications[].label
Regulation of Coronary Vasomotor Function by Oxidative Stress

# publications[].label
cAMP-independent dilation of coronary arterioles to adenosine : role of nitric oxide, G proteins, and K(ATP) channels.

# publications[].label
Resveratrol, a component of red wine, elicits dilation of isolated porcine retinal arterioles: role of nitric oxide and potassium channels.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
K-ATP channel-mediated coronary arteriolar dilation to adenosine is independent of cAMP

# publications[].label
Cardiac hypertrophy leads to coronary microvascular dysfunction and enhanced endothelin1/Rhokinase signaling in mice with transverse aortic coarctation (676.1)

# publications[].label
High glucose impairs EDHF-mediated dilation of coronary arterioles via reduced cytochrome P450 activity.

# publications[].label
Demonstration of eNOS uncoupling, altered proteinprotein association and impaired vascular reactivity:CRPmediated effects in vascular endothelium

# publications[].label
Adenosine-stimulated production of nitric oxide in porcine collateral-dependent coronary arterioles is enhanced by exercise training

# publications[].label
Temporal development of retinal arteriolar endothelial dysfunction in porcine type 1 diabetes.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Activation of Coronary Arteriolar PKC2 Impairs Endothelial NO-Mediated Vasodilation: Role of JNK/Rho Kinase Signaling and Xanthine Oxidase Activation.

# publications[].label
CORONARY VASOMOTOR REGULATION BY CRP AND LOX-1 RECEPTORS

# publications[].label
Safety and toxicity of nanomaterials for ocular drug delivery applications.

# publications[].label
Vasomotor regulation of coronary microcirculation by oxidative stress: role of arginase.

# publications[].label
Alterations of Ocular Hemodynamics Impair Ophthalmic Vascular and Neuroretinal Function.

# publications[].label
Effect of systemic administration of simvastatin on retinal circulation.

# publications[].label
Conducted remote dilations of isolated coronary arterioles require activation of barium-sensitive inward rectifier potassium channels

# publications[].label
VEGF Receptor-2-Linked PI3K/Calpain/SIRT1 Activation Mediates Retinal Arteriolar Dilations to VEGF and Shear Stress.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Morphological and pharmacological characterization of the porcine popliteal artery: A novel model for study of lower limb arterial disease.

# publications[].label
Exercise training enhances basal and VEGF-stimulated nitric oxide production in porcine collateral-dependent coronary arterioles

# publications[].label
Ischemia/reperfusion inhibits nitric oxide-mediated dilation in coronary arterioles by upregulation of arginase

# publications[].label
Mechanism of shear stress-induced coronary microvascular dilation

# publications[].label
Heterogeneous beta2-adrenoceptor expression and dilation in coronary arterioles across the left ventricular wall.

# publications[].label
Activation of angiotensin II AT1 receptor inhibits endothelium-dependent nitric oxide-mediated dilation of coronary arterioles: Role of superoxide anion

# publications[].label
Cold-acclimation leads to differential regulation of the steelhead trout (Oncorhynchus mykiss) coronary microcirculation.

# publications[].label
Effect of thrombin peptide TP508 on cultured endothelial cells suggest a wound healing mode of action that involves reversal of endothelial dysfunction

# publications[].label
ENDOTHELIN-1 AND CORONARY VASOMOTOR DYSFUNCTION: ROLE OF P38 KINASE AND NADPH OXIDASE

# publications[].label
Modulation of the Tryptophan Hydroxylase 1/Monoamine Oxidase-A/5-Hydroxytryptamine/5-Hydroxytryptamine Receptor 2A/2B/2C Axis Regulates Biliary Proliferation and Liver Fibrosis During Cholestasis.

# publications[].label
Vascular reactivity to calcitonin gene-related peptide is enhanced in subtotal nephrectomy-salt induced hypertension.

# publications[].label
Intravascular flow decreases erythrocyte consumption of nitric oxide.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Divergent roles of angiotensin II AT1 and AT2 receptors in modulating coronary microvascular function.

# publications[].label
Endothelin-1 Inhibits Endothelium-Dependent NO-Mediated Dilation of Coronary Arterioles via p38 Kinase Linked Superoxide Production From NAD(P)H Oxidase

# publications[].label
Intravitreal stanniocalcin-2 reduces photoreceptor degeneration and improves oscillatory potentials with elevated ocular blood flow in retinitis pigmentosa

# publications[].label
Heterogeneous coronary arteriolar dilation to beta(2)-adrenergic receptor activation: Role of nitric oxide and K-ATP channels

# publications[].label
Cellular signalling pathways mediating dilation of porcine pial arterioles to adenosine AA receptor activation.

# publications[].label
Role of Myocardial EndothelinConverting Enzyme in Promoting Coronary Arteriolar Constriction during Diabetes

# publications[].label
C-reactive protein inhibits endothelium-dependent NO-mediated dilation in coronary arterioles by activating p38 kinase and NAD(P)H oxidase.

# publications[].label
Dilation of retinal arterioles in response to lactate: role of nitric oxide, guanylyl cyclase, and ATP-sensitive potassium channels.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina.

# publications[].label
Requisite roles of A2A receptors, nitric oxide, and KATP channels in retinal arteriolar dilation in response to adenosine.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Endothelin-1 impairs coronary arteriolar dilation: Role of p38 kinase-mediated superoxide production from NADPH oxidase.

# publications[].label
Regulation of Coronary Vasomotor Function by Reactive Oxygen Species.

# publications[].label
Sildenafil (Viagra) evokes retinal arteriolar dilation: dual pathways via NOS activation and phosphodiesterase inhibition.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Adenosine A(2A) receptors mediate coronary microvascular dilation to adenosine: role of nitric oxide and ATP-sensitive potassium channels.

# publications[].label
Chronic hypoxia attenuates VEGF signaling and angiogenic responses by downregulation of KDR in human endothelial cells.

# publications[].label
oxLDL specifically impairs endothelium-dependent, NO-mediated dilation of coronary arterioles.

# publications[].label
A mathematical model for the distribution of hemodynamic parameters in the human retinal microvascular network

# publications[].label
Regulation of vasomotor function by integrins

# publications[].label
Differential progressive remodeling of coronary and cerebral arteries and arterioles in an aortic coarctation model of hypertension.

# publications[].label
Data on SD-OCT image acquisition, ultrastructural features, and horizontal tissue shrinkage in the porcine retina.

# publications[].label
Requisite roles of LOX-1, JNK, and arginase in diabetes-induced endothelial vasodilator dysfunction of porcine coronary arterioles.

# publications[].label
Functional and molecular characterization of receptor subtypes mediating coronary microvascular dilation to adenosine.

# publications[].label
Functional and molecular characterization of the endothelin system in retinal arterioles.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
TP508 (Chrysalin) reverses endothelial dysfunction and increases perfusion and myocardial function in hearts with chronic ischemia.

# publications[].label
Constriction of retinal arterioles to endothelin-1: requisite role of rho kinase independent of protein kinase C and L-type calcium channels.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Impaired nitric oxide-mediated coronary arteriolar dilation in hypertrophic cardiomyopathy: role of angiotensin-II and oxidative stress

# publications[].label
Constitutive expression of arginase in microvascular endothelial cells counteracts nitric oxide-mediated vasodilatory function.

# publications[].label
Upregulation of arginase by H2O2 impairs endothelium-dependent nitric oxide-mediated dilation of coronary arterioles.

# publications[].label
Functional and molecular evidence of adenosine A(2A) receptor in coronary arteriolar dilation to adenosine

# publications[].label
Isoflurane-induced dilation of porcine coronary microvessels is endothelium dependent and inhibited by glibenclamide.

# publications[].label
Human C-reactive protein induces endothelial dysfunction and uncoupling of eNOS in vivo.

# publications[].label
Functional and molecular studies of adenosine receptors and K-ATP channels in the coronary microcirculation

# publications[].label
Organ-on-chips made of blood: endothelial progenitor cells from blood reconstitute vascular thromboinflammation in vessel-chips.

# publications[].label
Hyperglycemia Enhances Constriction of Retinal Venules via Activation of the Reverse-Mode Sodium-Calcium Exchanger.

# publications[].label
Intravitreal Administration of Stanniocalcin-1 Rescues Photoreceptor Degeneration with Reduced Oxidative Stress and Inflammation in a Porcine Model of Retinitis Pigmentosa.

# publications[].label
Hyperglycemia Enhances Constriction of Retinal Venules to Endothelin1 via Activation of the ReverseMode SodiumCalcium Exchanger

# publications[].label
Oxidized low-density lipoprotein inhibits nitric oxide-mediated coronary arteriolar dilation by up-regulating endothelial arginase I.

# publications[].label
Ischemia-reperfusion selectively impairs nitric oxide-mediated dilation in coronary arterioles: counteracting role of arginase.

# publications[].label
Rho kinase-mediated coronary arteriolar constriction to endothelin-1: Mechanistic implications for cardiac syndrome X

# publications[].label
Retinal arteriolar responses to acute severe elevation in systemic blood pressure in cats: role of endothelium-derived factors.

# publications[].label
Selective Activation of ROCK2 Isoform Contributes to Vasomotor Regulation of Retinal Arterioles

# publications[].label
Control of the coronary microcirculation in steelhead trout (Oncorhynchus mykiss)

# publications[].label
Effects of histidine-rich glycoprotein on cerebral blood vessels.

# publications[].label
Intravitreal Stanniocalcin-1 Enhances New Blood Vessel Growth in a Rat Model of Laser-Induced Choroidal Neovascularization.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Modulation of nitric oxide bioavailability by erythrocytes.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Newly Identified Peptide, Peptide Lv, Promotes Pathological Angiogenesis.

# researcherOn[].label
Vasomotor Dysfunction of Retinal Arterioles in Diabetes

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].label
Intravitreal ECE-1 siRNA Treatment for Retinal Dysfunction during Early Diabetes

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].label
Effects of Microgravity on Ocular Vascular Hydrodynamics

# researcherOn[].awardedBy.label
National Aeronautics and Space Administration - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Temporal impact of simulated microgravity on ocular vascular hydrodynamics

# researcherOn[].awardedBy.label
Ames Research Center - (Mountain View, California, United States)

# researcherOn[].label
Neuroendocrine Regulation of Biliary Growth and Fibrosis

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Diabetes and Digestive and Kidney Disorders

# researcherOn[].label
Roles of LOX-1 and Stress-Activated Kinases in Retinal Dysfunction during Early Diabetes

# researcherOn[].awardedBy.label
United States Department of Health and Human Services - (Washington D.C., District of Columbia, United States)

# streetAddress
Texas A&M Health Science Center; Medical Physiology; 1114 TAMUS

# organizations[]
Texas A&M University Naresh K. Vashisht College of Medicine

# schools[]
Texas A&M University Naresh K. Vashisht College of Medicine