# overview
The Begley Group is interested in the mechanistic chemistry and enzymology of complex organic transformations, particularly those found on the vitamin biosynthetic pathways. We are currently working on the biosynthesis of thiamin, molybdopterin, pyridoxal phosphate and menaquinone. Our research involves a combination of molecular biology, protein biochemistry, organic synthesis and structural studies and provides a strong training for students interested in understanding the organic chemistry of living systems and in pursuing careers in biotechnology, drug design or academia.

Thiamin pyrophosphate plays a key role in the stabilization of the acyl carbanion synthon in carbohydrate and amino acid metabolism. The biosyntheses of the thiamin pyrimidine and thiazole are complex and are different from any of the characterized chemical or biochemical routes to these heterocycles. We are particularly interested in cellular physiology and the mechanistic enzymology of thiamin biosynthesis. As an example of one of the complex transformations on this pathway, the figure below shows the structure of the pyrimidine synthase catalyzing the complex rearrangement of aminoimidazole ribotide (left) to the thiamin pyrimidine (right).

# keywords[]
Oxidoreductases Acting On Ch-ch Group Donors

# keywords[]
3404 Medicinal And Biomolecular Chemistry

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3404 Medicinal And Biomolecular Chemistry

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5 Development Of Treatments And Therapeutic Interventions

# educationAndTraining[].organization.label
California Institute of Technology - (Pasadena, California, United States)

# educationAndTraining[].organization.label
National University of Ireland - (Dublin, Ireland)

# awardsAndHonors[].label
Repligen Award in Chemistry of Biological Processes

# publications[].label
The crystal structure and mechanism of orotidine 5'-monophosphate decarboxylase.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS.

# publications[].label
Tryptophan Lyase (NosL): Mechanistic Insights from Substrate Analogues and Mutagenesis.

# publications[].label
Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae.

# publications[].label
Globally Important Haptophyte Algae Use Exogenous Pyrimidine Compounds More Efficiently than Thiamin.

# publications[].label
Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5'-phosphate.

# publications[].label
Biosynthesis of the thioquinolobactin siderophore: an interesting variation on sulfur transfer.

# publications[].label
PHENOXAZINONE SYNTHASE - ENZYMATIC CATALYSIS OF AN AMINOPHENOL OXIDATIVE CASCADE

# publications[].label
From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.

# publications[].label
Mechanistic studies on prolyl-4-hydroxylase: the vitamin C requiring uncoupled oxidation.

# publications[].label
Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Simultaneous kinetic characterization of multiple protein forms by top down mass spectrometry.

# publications[].label
Mechanism-based inactivation of the human prolyl-4-hydroxylase by 5-oxaproline-containing peptides: Evidence for a prolyl radical intermediate

# publications[].label
Crystal structure of thiaminase-I from Bacillus thiaminolyticus at 2.0 A resolution.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: Identification of a novel acyl disulfide linked protein-protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

# publications[].label
Vitamin B1 synthesis and salvage in Plasmodium falciparum.

# publications[].label
The biosynthesis of coenzyme A in bacteria.

# publications[].label
Mechanistic studies on thiaminase I: Identification of the product of thiamin degradation in the absence of the nucleophilic cosubstrate

# publications[].label
Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate.

# publications[].label
Solution structure of ThiS and implications for the evolutionary roots of ubiquitin.

# publications[].label
Alternatives to vitamin B1 uptake revealed with discovery of riboswitches in multiple marine eukaryotic lineages.

# publications[].label
Biosynthesis of the thiamin pyrimidine: the reconstitution of a remarkable rearrangement reaction.

# publications[].label
Reagent for the detection of protein thiocarboxylates in the bacterial proteome: lissamine rhodamine B sulfonyl azide.

# publications[].label
Substrate analogues as mechanistic probes of methyl-S-coenzyme M reductase.

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Biochemical Characterization and Structural Basis of Reactivity and Regioselectivity Differences between Burkholderia thailandensis and Burkholderia glumae 1,6-Didesmethyltoxoflavin N-Methyltransferase.

# publications[].label
A "radical dance" in thiamin biosynthesis: mechanistic analysis of the bacterial hydroxymethylpyrimidine phosphate synthase.

# publications[].label
Mechanistic Studies on Thiaminase I. 3. Stereochemistry of the Thiaminase I and the Bisulfite-Catalyzed Degradation of Chiral Monodeuteriothiamin.

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Crystal structure of Mycobacterium tuberculosis polyketide synthase 11 (PKS11) reveals intermediates in the synthesis of methyl-branched alkylpyrones.

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SYNTHESIS OF THE DINUCLEOTIDE SPORE PHOTOPRODUCT

# publications[].label
Mechanistic studies on phosphopantothenoylcysteine decarboxylase: trapping of an enethiolate intermediate with a mechanism-based inactivating agent.

# publications[].label
Biosynthesis of thiamin thiazole: determination of the regiochemistry of the S/O acyl shift by using 1,4-dideoxy-D-xylulose-5-phosphate.

# publications[].label
Spore photoproduct lyase from Bacillus subtilis spores is a novel iron-sulfur DNA repair enzyme which shares features with proteins such as class III anaerobic ribonucleotide reductases and pyruvate-formate lyases.

# publications[].label
Mechanistic studies of a protonolytic organomercurial cleaving enzyme: bacterial organomercurial lyase.

# publications[].label
Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry.

# publications[].label
Aminofutalosine Synthase: Evidence for Captodative and Aryl Radical Intermediates Using -Scission and SRN1 Trapping Reactions.

# publications[].label
Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5'-phosphate biosynthesis.

# publications[].label
Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene.

# publications[].label
Comparison of thiaminase activity in fish using the radiometric and 4-nitrothiophenol colorimetric methods.

# publications[].label
The biosynthesis and degradation of thiamin (vitamin B1).

# publications[].label
Crystal structure and mechanism of tryptophan 2,3-dioxygenase, a heme enzyme involved in tryptophan catabolism and in quinolinate biosynthesis.

# publications[].label
Biosynthesis of dimethylbenzimidazole: Catalysis of a remarkable flavin degradation reaction

# publications[].label
Comprehensive Natural Products II Chemistry and Biology Overview and Introduction

# publications[].label
Alkylcysteine Sulfoxide C-S Monooxygenase Uses a Flavin-Dependent Pummerer Rearrangement.

# publications[].label
A 5-formyltetrahydrofolate cycloligase paralog from all domains of life: comparative genomic and experimental evidence for a cryptic role in thiamin metabolism.

# publications[].label
Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.

# publications[].label
Structural studies on 3-hydroxyanthranilate-3,4-dioxygenase: the catalytic mechanism of a complex oxidation involved in NAD biosynthesis.

# publications[].label
New cysteine biosynthetic pathway in mycobacterium tuberculosis.

# publications[].label
Structural characterization of the enzyme-substrate, enzyme-intermediate, and enzyme-product complexes of thiamin phosphate synthase.

# publications[].label
Fast, efficient localization of protein sequence error and covalent modification by high resolution tandem mass spectrometry.

# publications[].label
Mechanistic Studies on the Single-Turnover Yeast Thiamin Pyrimidine Synthase: Characterization of the Inactive Enzyme.

# publications[].label
The structural and biochemical foundations of thiamin biosynthesis.

# publications[].label
U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems.

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Biocatalysis and biotransformation enzymology in the genomics era - Editorial overview

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Synthesis of P-32-labeled intermediates on the purine biosynthetic pathway

# publications[].label
Molybdopterin biosynthesis: trapping an unusual purine ribose adduct in the MoaA-catalyzed reaction.

# publications[].label
Reversal of the substrate specificity of CMP N-glycosidase to dCMP.

# publications[].label
The selectivity for cysteine over serine in coenzyme A biosynthesis.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .4. THE ENTHALPY OF CLEAVAGE OF A MODEL PHOTODIMER

# publications[].label
RutA-Catalyzed Oxidative Cleavage of the Uracil Amide Involves Formation of a Flavin-N5-oxide.

# publications[].label
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.

# publications[].label
Domain organization in Candida glabrata THI6, a bifunctional enzyme required for thiamin biosynthesis in eukaryotes.

# publications[].label
Phosphopantothenoylcysteine synthetase from Escherichia coli. Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria.

# publications[].label
Chemical biology: an educational challenge for chemistry departments.

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Ligand-induced structural and dynamic changes in the C-Src SH3 domain

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Menaquinone Biosynthesis: New Strategies to Trap Radical Intermediates in the MqnE-Catalyzed Reaction.

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Structural biology of enzymes of the thiamin biosynthesis pathway.

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Selective deprotection of tert-butyldimethylsilyl ether with lithium bromide and 18-crown-6

# publications[].label
Tryptophan catabolism: identification and characterization of a new degradative pathway.

# publications[].label
Anaerobic biosynthesis of the lower ligand of vitamin B12.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.

# publications[].label
MECHANISTIC STUDIES ON PROTOCHLOROPHYLLIDE REDUCTASE - A MODEL SYSTEM FOR THE ENZYMATIC-REACTION

# publications[].label
Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism.

# publications[].label
Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.

# publications[].label
Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.

# publications[].label
13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase.

# publications[].label
The vitamin B1 metabolism of Staphylococcus aureus is controlled at enzymatic and transcriptional levels.

# publications[].label
Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster.

# publications[].label
Inhibitors of pantothenate kinase: novel antibiotics for staphylococcal infections.

# publications[].label
Formation of the dimethylbenzimidazole ligand of coenzyme B(12) under physiological conditions by a facile oxidative cascade.

# publications[].label
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.

# publications[].label
Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis.

# publications[].label
DESIGN OF A CLEAVABLE LINKER FOR THE SYNTHESIS OF A CIS-SYN PYRIMIDINE PHOTODIMER

# publications[].label
Biochemistry: Origin of a key player in methane biosynthesis (vol 543, pg 49, 2017)

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Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily.

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NAD biosynthesis: identification of the tryptophan to quinolinate pathway in bacteria.

# publications[].label
The pyridine ring of NAD is formed by a nonenzymatic pericyclic reaction.

# publications[].label
Pseudouridine monophosphate glycosidase: a new glycosidase mechanism.

# publications[].label
Tryptophan catabolism: Gene identification and mechanistic studies

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .5. SECONDARY DEUTERIUM-ISOTOPE EFFECTS ON THE CLEAVAGE OF THE URACIL PHOTODIMER RADICAL CATION AND ANION

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DNA-protein cross-linking: model systems for pyrimidine-aromatic amino acid cross-linking.

# publications[].label
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.

# publications[].label
Catalysis of a flavoenzyme-mediated amide hydrolysis.

# publications[].label
Biochemistry: Origin of a key player in methane biosynthesis.

# publications[].label
Comparison of femtosecond laser and continuous wave UV sources for protein-nucleic acid crosslinking.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).

# publications[].label
Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamin biosynthesis in Bacillus subtilis.

# publications[].label
Phosphomethylpyrimidine Synthase (ThiC): Trapping of Five Intermediates Provides Mechanistic Insights on a Complex Radical Cascade Reaction in Thiamin Biosynthesis.

# publications[].label
Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: Identification of a novel acyldisulfide linked protein-protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

# publications[].label
Molybdopterin biosynthesis: trapping of intermediates for the MoaA-catalyzed reaction using 2'-deoxyGTP and 2'-chloroGTP as substrate analogues.

# publications[].label
Stoichiometry and spectroscopic identity of copper centers in phenoxazinone synthase: a new addition to the blue copper oxidase family.

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Biosynthesis of a thiamin antivitamin in Clostridium botulinum.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .9. IS THE CLEAVAGE OF THE CYCLOBUTANE PYRIMIDINE PHOTODIMER RADICAL-ANION A PERICYCLIC REACTION

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Competence of Thiamin Diphosphate-Dependent Enzymes with 2'-Methoxythiamin Diphosphate Derived from Bacimethrin, a Naturally Occurring Thiamin Anti-vitamin.

# publications[].label
NMR structure of the E-coli thiamine biosynthesis pathway protein ThiS

# publications[].label
Vitamin B₂ Catabolism: Nature's Route from Riboflavin to Acetoacetate and Pyruvate

# publications[].label
Insights into the initial steps of the thiamin pyrimidine synthase (ThiC)-catalyzed reaction through EPR spectroscopic characterization of radical intermediates.

# publications[].label
An assay for thiaminase I in complex biological samples.

# publications[].label
Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex.

# publications[].label
Structure of oxalate decarboxylase from Bacillus subtilis at 1.75 A resolution.

# publications[].label
Identification of modification sites in large biomolecules by stable isotope labeling and tandem high resolution mass spectrometry. The active site nucleophile of thiaminase I.

# publications[].label
Radical S-adenosylmethionine (SAM) enzymes in cofactor biosynthesis: a treasure trove of complex organic radical rearrangement reactions.

# publications[].label
BIOL 155-Mechanistic studies on pyridoxal phosphate synthase: The reaction pathway leading to a chromophoric intermediate

# publications[].label
Detection of four oxidation sites in viral prolyl-4-hydroxylase by top-down mass spectrometry.

# publications[].label
The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase.

# publications[].label
Oxidative Dearomatization of PLP in Thiamin Pyrimidine Biosynthesis in Candida albicans.

# publications[].label
Molybdopterin biosynthesis-Mechanistic studies on a novel MoaA catalyzed insertion of a purine carbon into the ribose of GTP.

# publications[].label
One enzyme for two radical reactions: Deciphering the mechanism of a novel radical SAM enzyme critical for F-420 cofactor biosynthesis

# publications[].label
Biosynthesis of F0, precursor of the F420 cofactor, requires a unique two radical-SAM domain enzyme and tyrosine as substrate.

# publications[].label
Thiaminase I (42 kDa) heterogeneity, sequence refinement, and active site location from high-resolution tandem mass spectrometry.

# publications[].label
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis.

# publications[].label
Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation.

# publications[].label
An Aminoimidazole Radical Intermediate in the Anaerobic Biosynthesis of the 5,6-Dimethylbenzimidazole Ligand to Vitamin B12.

# publications[].label
Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.

# publications[].label
Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate.

# publications[].label
The structural basis for the remarkable catalytic proficiency of orotidine 5'-monophosphate decarboxylase.

# publications[].label
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.

# publications[].label
The antibiotic activity of N-pentylpantothenamide results from its conversion to ethyldethia-coenzyme a, a coenzyme a antimetabolite.

# publications[].label
Tryptophan Lyase (NosL): A Cornucopia of 5'-Deoxyadenosyl Radical Mediated Transformations.

# publications[].label
Oxidative cascades: a facile biosynthetic strategy for the assembly of complex molecules.

# publications[].label
Two-dimensional mass spectrometry of biomolecules at the subfemtomole level.

# publications[].label
Menaquinone Biosynthesis: Biochemical and Structural Studies of Chorismate Dehydratase.

# publications[].label
A genetic screen for the identification of thiamin metabolic genes.

# publications[].label
The selectivity for cysteine over serine in coenzyme A biosynthesis (vol 6, pg 284, 2005)

# publications[].label
Cofactor biosynthesis: an organic chemist's treasure trove.

# publications[].label
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.

# publications[].label
Identification of the product of toxoflavin lyase: degradation via a Baeyer-Villiger oxidation.

# publications[].label
Hexachlorobenzene Catabolism Involves a Nucleophilic Aromatic Substitution and Flavin-N5-Oxide Formation.

# publications[].label
Structure of trifunctional THI20 from yeast.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Sensitivity of breast cancer cell lines to recombinant thiaminase I.

# publications[].label
Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase.

# publications[].label
Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium tuberculosis.

# publications[].label
Comparative genomics in enzymology: Thiamin, cysteine and nad biosynthesis.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Retraction notice to "Comparison of thiaminase activity in fish using the radiometric and 4-nitrothiophenol colorimetric methods" [J. Great Lakes Res. 36 (2010) 641-645].

# publications[].label
Construction of a thiamin sensor from the periplasmic thiamin binding protein.

# publications[].label
Thiamin biosynthesis: still yielding fascinating biological chemistry.

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Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation.

# publications[].label
BIOSYNTHESIS OF THIAMIN-I - THE FUNCTION OF THE THIE GENE-PRODUCT

# publications[].label
The production of paclitaxel and other taxanes in Taxus canadensis suspension cell cultures elicited with methyl jasmonate.

# publications[].label
Thiamin biosynthesis in eukaryotes: characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis.

# publications[].label
Mechanistic Studies on Prolyl-4-Hydroxylase: Demonstration That the Ferryl Intermediate Does Not Exchange with Water.

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A Remarkable Oxidative Cascade That Replaces the Riboflavin C8 Methyl with an Amino Group during Roseoflavin Biosynthesis.

# publications[].label
Structure of the PLP degradative enzyme 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti MAFF303099 and its mechanistic implications.

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PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .3. THE TRAPPING OF THE ONE-BOND-CLEAVED INTERMEDIATE FROM A PHOTODIMER RADICAL CATION MODEL SYSTEM

# publications[].label
The enzymology of sulfur activation during thiamin and biotin biosynthesis.

# publications[].label
Iterative Methylations Resulting in the Biosynthesis of the t-Butyl Group Catalyzed by a B12-Dependent Radical SAM Enzyme in Cystobactamid Biosynthesis.

# publications[].label
Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

# publications[].label
Thi20, a remarkable enzyme from Saccharomyces cerevisiae with dual thiamin biosynthetic and degradation activities.

# publications[].label
Mechanistic studies on DNA photolyase, II. Is the blue enzyme isolated from Escherichia coli a mutant?

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Toxoflavin lyase requires a novel 1-His-2-carboxylate facial triad.

# publications[].label
Investigations into the Biosynthesis, Regulation, and Self-Resistance of Toxoflavin in Pseudomonas protegens Pf-5.

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Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3 A resolution.

# publications[].label
Crystallization and preliminary X-ray analysis of thiaminase I from Bacillus thiaminolyticus: space group change upon freezing of crystals.

# publications[].publisher
International Union of Crystallography (IUCr)

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O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.

# publications[].label
Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis (vol 13, pg 290, 2017)

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PHOTOENZYMES - A NOVEL CLASS OF BIOLOGICAL CATALYSTS

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An efficient enzymatic synthesis of thiamin pyrophosphate.

# publications[].label
Biosynthesis of Branched Alkoxy Groups: Iterative Methyl Group Alkylation by a Cobalamin-Dependent Radical SAM Enzyme.

# publications[].label
Mechanistic studies on thiamin formation and degradation: thiaminase I and thiamin phosphate synthase

# publications[].label
Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis.

# publications[].label
Mechanistic Studies on CysS - A Vitamin B12-Dependent Radical SAM Methyltransferase Involved in the Biosynthesis of the tert-Butyl Group of Cystobactamid.

# publications[].label
Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction.

# publications[].label
Catalysis of a new ribose carbon-insertion reaction by the molybdenum cofactor biosynthetic enzyme MoaA.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .1. SECONDARY DEUTERIUM-ISOTOPE EFFECTS ON THE CLEAVAGE OF 2'-DEOXYURIDINE DINUCLEOTIDE PHOTODIMERS

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Formation of the chromophore of the pyoverdine siderophores by an oxidative cascade.

# publications[].label
Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis.

# publications[].label
Mechanistic Studies on the Radical SAM Enzyme Tryptophan Lyase (NosL).

# publications[].label
Anaerobic 5-Hydroxybenzimidazole Formation from Aminoimidazole Ribotide: An Unanticipated Intersection of Thiamin and Vitamin B Biosynthesis.

# publications[].label
Trapping and Electron Paramagnetic Resonance Characterization of the 5'dAdo Radical in a Radical S-Adenosyl Methionine Enzyme Reaction with a Non-Native Substrate.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE-VIII - STUDIES ON THE FRAGMENTATION OF THE RADICAL-ANION AND CATION OF A URACIL-ALKENE PHOTOADDUCT

# publications[].label
In vitro reconstitution of the radical S-adenosylmethionine enzyme MqnC involved in the biosynthesis of futalosine-derived menaquinone.

# publications[].label
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.

# publications[].label
Mechanistic studies on thiaminase I. Overexpression and identification of the active site nucleophile.

# publications[].label
Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis.

# publications[].label
Thiamin: A simple vitamin with a complex biosynthetic pathway

# publications[].label
Thiamine deficiency in Cambodian infants with and without beriberi.

# publications[].label
The biosynthesis of nicotinamide adenine dinucleotides in bacteria.

# publications[].label
Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution.

# publications[].label
A novel mechanism of sulfur transfer catalyzed by O-acetylhomoserine sulfhydrylase in the methionine-biosynthetic pathway of Wolinella succinogenes.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Cysteine Dealkylation in Bacillus subtilis by a Novel Flavin-Dependent Monooxygenase.

# publications[].label
Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics.

# publications[].label
A NEW SYNTHESIS OF 1-DEOXY-D-THREO-2-PENTULOSE, A BIOSYNTHETIC PRECURSOR TO THE THIAZOLE MOIETY OF THIAMIN

# publications[].label
Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae.

# publications[].label
Structural Studies of Quinolinate Synthase

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Structural studies of thiamin monophosphate kinase in complex with substrates and products.

# publications[].label
PICOSECOND LASER PHOTOLYSIS STUDIES ON THE PHOTOREPAIR OF PYRIMIDINE DIMERS BY DNA PHOTOLYASE .1. LASER PHOTOLYSIS OF PHOTOLYASE-2-DEOXYURIDINE DINUCLEOTIDE PHOTODIMER COMPLEX

# publications[].label
Structural similarities between thiamin-binding protein and thiaminase-I suggest a common ancestor.

# publications[].label
Solid-state NMR and density functional theory studies of ionization states of thiamin.

# publications[].label
Antibacterial Strategy against H. pylori: Inhibition of the Radical SAM Enzyme MqnE in Menaquinone Biosynthesis.

# publications[].label
Vitamin B1 de novo synthesis in the human malaria parasite Plasmodium falciparum depends on external provision of 4-amino-5-hydroxymethyl-2-methylpyrimidine.

# publications[].label
High-resolution crystal structure of the eukaryotic HMP-P synthase (THIC) from Arabidopsis thaliana.

# publications[].label
Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase.

# publications[].label
Cloning, sequencing and regulation of thiA, a thiamin biosynthesis gene from Bacillus subtilis.

# publications[].label
Investigation of the Iterative Methylations by a Cobalamin-Dependent Radical SAM Methyltransferase

# publications[].label
beta-scission of the N-O bond in alkyl hydroxamate radicals: a fast radical trap.

# publications[].label
Dibenzothiophene Catabolism Proceeds via a Flavin-N5-oxide Intermediate.

# publications[].label
Phenoxazinone synthase: mechanism for the formation of the phenoxazinone chromophore of actinomycin.

# publications[].label
Aminofutalosine Synthase (MqnE): A New Catalytic Motif in Radical SAM Enzymology.

# publications[].label
Structural insights into the mechanism of the PLP synthase holoenzyme from Thermotoga maritima.

# publications[].label
The mechanism of action of bacimethrin, a naturally occurring thiamin antimetabolite.

# publications[].label
Cofactor biosynthesis: A mechanistic perspective

# publications[].label
Top down characterization of larger proteins (45 kDa) by electron capture dissociation mass spectrometry.

# publications[].label
Menaquinone biosynthesis: formation of aminofutalosine requires a unique radical SAM enzyme.

# publications[].label
PROTOCHLOROPHYLLIDE REDUCTASE .1. DETERMINATION OF THE REGIOCHEMISTRY AND THE STEREOCHEMISTRY OF THE REDUCTION OF PROTOCHLOROPHYLLIDE TO CHLOROPHYLLIDE

# publications[].label
PHENOXAZINONE SYNTHASE - ENZYMATIC CATALYSIS OF AN AMINOPHENOL OXIDATIVE CASCADE

# publications[].label
HMP binding protein ThiY and HMP-P synthase THI5 are structural homologues.

# publications[].label
RNA-seq analysis reveals that an ECF  factor, AcsS, regulates achromobactin biosynthesis in Pseudomonas syringae pv. syringae B728a.

# publications[].label
The iron responsive sigma factor, AcsS, responsible for regulation of achromobactin biosynthesis in Pseudomonas syringae pv. syringae B728a

# publications[].label
Mechanistic studies on the biosynthesis and degradation of thiamin

# publications[].label
Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.

# publications[].label
Characterization of TenA from Bacillus subtilis: A Thiaminase II

# publications[].publisher
International Union of Crystallography (IUCr)

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Thiamin phosphate synthase: the rate of pyrimidine carbocation formation.

# publications[].label
Mechanistic Studies on Tryptophan Lyase (NosL): Identification of Cyanide as a Reaction Product.

# publications[].label
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.

# publications[].label
Top down characterization of secreted proteins from Mycobacterium tuberculosis by electron capture dissociation mass spectrometry.

# publications[].label
Mechanistic studies on thiaminase I .1. The stereochemical course of the reaction

# publications[].label
A maize thiamine auxotroph is defective in shoot meristem maintenance.

# publications[].label
Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis

# publications[].label
Mechanistic studies on the repair of a novel DNA photolesion: the spore photoproduct.

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Glycal formation in crystals of uridine phosphorylase.

# publications[].label
Structure of a Clostridium botulinum C143S thiaminase I/thiamin complex reveals active site architecture .

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A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.

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Enzymatic reactions involving novel mechanisms of carbanion stabilization.

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Flavin-N5-oxide: A new, catalytic motif in flavoenzymology.

# publications[].label
Functional copper at the acetyl-CoA synthase active site.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Active-site models for complexes of quinolinate synthase with substrates and intermediates.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099.

# researcherOn[].label
Menaquinone Biosynthesis via the Futalosine Pathway

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Mentoring for the Future In Academic-Chemistry

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].label
New Radical SAM Enzymes Involved in Menaquinone biosynthesis

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
The Mechanistic Enzymology of Thiamin Biosynthesis

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Diabetes and Digestive and Kidney Disorders

# streetAddress
Texas A&M University; Chemistry Department; 3255 TAMU

# inTheNews[].label
The important role of the Young Scientist Exhibition

# inTheNews[].label
Antivitamins may soon replace antibiotics. Here's why

# inTheNews[].label
Are antivitamins the new antibiotics? Researchers develops drug approach against bacterial infections

# etdChairOf[].label
Chanani, Prem Kumar (2017-12). Novel Flavin Chemistry Involved in the Biosynthesis of the Lower Ligand of Vitamin B12. Doctoral Dissertation.

# etdChairOf[].label
Mehta, Angad P (2015-05). Mechanistic Studies on the Radical S-Adenosylmethionine Enzymes Involved in Molybdopterin, Thiamin and Vitamin B12 Biosynthesis. Doctoral Dissertation.

# etdChairOf[].label
Lai, Rung Yi (2015-08). The Mechanistic Investigation of the Bacterial Thiamin Thiazole Synthase and the Eukaryotic Thiamin Pyrimidine Synthase. Doctoral Dissertation.

# etdChairOf[].label
Jhulki, Isita (2020-05). Mechanistic Studies on a Remarkable Flavoenzyme Involved in Roseoflavin Biosynthesis. Doctoral Dissertation.

# etdChairOf[].label
Bhandari, Dhananjay (2017-12). Studies on the Flavin-mediated Cysteine Salvage Pathway, Structural Enzymology of Riboflavin Lyase and Mechanistic Investigation of the Radical S-adenosyl-L-methionine-mediated Tryptophan Lyase. Doctoral Dissertation.

# etdChairOf[].label
Xie, Lina (2018-12). Enzyme-Catalyzed Acylium Ion Formation and  Reagents for the Detection of Thiocarboxylates Involved in the  Biosynthesis of the NAD Derived Pincer Cofactor. Doctoral Dissertation.

# etdChairOf[].label
Liu, Yiquan (2015-12). Development of Chemical Strategies for Specifically Probing and Identifying Sulfur Carrier Proteins and Vitamin B6-Dependent Enzymes in Bacteria. Doctoral Dissertation.

# etdChairOf[].label
Mahanta, Nilkamal (2015-05). Studies on the Biosynthesis of Menaquinone (Vitamin K) and on the Catabolism of Pseudouridine. Doctoral Dissertation.

# etdChairOf[].label
Joshi, Sumedh Varad (2019-12). MECHANISTIC STUDIES ON RADICAL S-ADENOSYL-L-METHIONINE ENZYMES INVOLVED IN MENAQUINONE, THIAMIN AND COBALAMIN BIOSYNTHESIS. Doctoral Dissertation.

# etdChairOf[].label
Wang, Yuanyou (2019-05). Mechanistic Studies of Radical SAM Enzymes in Cystobactamid, Anaerobilin and Thiamin Biosynthesis. Doctoral Dissertation.

# etdChairOf[].label
Messer, Kayla J. (2011-05). Pyridoxal Phosphate as a Tag to  Identify Enzymes Within the “PLP-ome”. Master's Thesis.

# etdChairOf[].label
Adak, Sanjoy (2019-12). Identification of New Catalytic Motifs in Flavoenzymes and Mechanistic Studies of a Cofactor Independent Oxygenase. Doctoral Dissertation.

# etdChairOf[].label
Chakrabarty, Yindrila (2016-08). Riboflavin Lyase: An Intriguing Flavoenzyme Involved in Riboflavin Degradation. Doctoral Dissertation.

# etdChairOf[].label
Olivard, Sarah (2012-12). Studies on Uptake of Thiamin Analogs by a Thiamin Deficient E. coli Mutant Strain. Master's Thesis.

# etdChairOf[].label
Shome, Brateen (2017-05). Synthetic and Biochemical Exploration of the Degradation and Utilization of Thiamin Analogs and Preliminary Studies on Methanopterin methyltransferase. Doctoral Dissertation.