# overview
The Begley Group is interested in the mechanistic chemistry and enzymology of complex organic transformations, particularly those found on the vitamin biosynthetic pathways. We are currently working on the biosynthesis of thiamin, molybdopterin, pyridoxal phosphate and menaquinone. Our research involves a combination of molecular biology, protein biochemistry, organic synthesis and structural studies and provides a strong training for students interested in understanding the organic chemistry of living systems and in pursuing careers in biotechnology, drug design or academia.

Thiamin pyrophosphate plays a key role in the stabilization of the acyl carbanion synthon in carbohydrate and amino acid metabolism. The biosyntheses of the thiamin pyrimidine and thiazole are complex and are different from any of the characterized chemical or biochemical routes to these heterocycles. We are particularly interested in cellular physiology and the mechanistic enzymology of thiamin biosynthesis. As an example of one of the complex transformations on this pathway, the figure below shows the structure of the pyrimidine synthase catalyzing the complex rearrangement of aminoimidazole ribotide (left) to the thiamin pyrimidine (right).

# keywords[]
3404 Medicinal And Biomolecular Chemistry

# keywords[]
5 Development Of Treatments And Therapeutic Interventions

# keywords[]
3404 Medicinal And Biomolecular Chemistry

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Oxidoreductases Acting On Ch-ch Group Donors

# educationAndTraining[].organization.label
California Institute of Technology - (Pasadena, California, United States)

# educationAndTraining[].organization.label
National University of Ireland - (Dublin, Ireland)

# awardsAndHonors[].label
Repligen Award in Chemistry of Biological Processes

# publications[].label
Comparison of thiaminase activity in fish using the radiometric and 4-nitrothiophenol colorimetric methods.

# publications[].label
SYNTHESIS OF THE DINUCLEOTIDE SPORE PHOTOPRODUCT

# publications[].label
In vitro reconstitution of the radical S-adenosylmethionine enzyme MqnC involved in the biosynthesis of futalosine-derived menaquinone.

# publications[].label
Thiamin biosynthesis in eukaryotes: characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis.

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The biosynthesis and degradation of thiamin (vitamin B1).

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Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution.

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The biosynthesis of nicotinamide adenine dinucleotides in bacteria.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE-VIII - STUDIES ON THE FRAGMENTATION OF THE RADICAL-ANION AND CATION OF A URACIL-ALKENE PHOTOADDUCT

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BIOSYNTHESIS OF THIAMIN-I - THE FUNCTION OF THE THIE GENE-PRODUCT

# publications[].label
Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase.

# publications[].label
The production of paclitaxel and other taxanes in Taxus canadensis suspension cell cultures elicited with methyl jasmonate.

# publications[].label
Comparative genomics in enzymology: Thiamin, cysteine and nad biosynthesis.

# publications[].label
A 5-formyltetrahydrofolate cycloligase paralog from all domains of life: comparative genomic and experimental evidence for a cryptic role in thiamin metabolism.

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Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis.

# publications[].label
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis.

# publications[].label
Domain organization in Candida glabrata THI6, a bifunctional enzyme required for thiamin biosynthesis in eukaryotes.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: Identification of a novel acyldisulfide linked protein-protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

# publications[].label
RNA-seq analysis reveals that an ECF  factor, AcsS, regulates achromobactin biosynthesis in Pseudomonas syringae pv. syringae B728a.

# publications[].label
Mechanistic Studies on the Radical SAM Enzyme Tryptophan Lyase (NosL).

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Mechanistic studies on prolyl-4-hydroxylase: the vitamin C requiring uncoupled oxidation.

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Biocatalysis and biotransformation enzymology in the genomics era - Editorial overview

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Vitamin B1 synthesis and salvage in Plasmodium falciparum.

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Formation of the dimethylbenzimidazole ligand of coenzyme B(12) under physiological conditions by a facile oxidative cascade.

# publications[].label
Mechanistic studies on thiaminase I .1. The stereochemical course of the reaction

# publications[].label
An Aminoimidazole Radical Intermediate in the Anaerobic Biosynthesis of the 5,6-Dimethylbenzimidazole Ligand to Vitamin B12.

# publications[].label
High-resolution crystal structure of the eukaryotic HMP-P synthase (THIC) from Arabidopsis thaliana.

# publications[].label
Molybdopterin biosynthesis: trapping of intermediates for the MoaA-catalyzed reaction using 2'-deoxyGTP and 2'-chloroGTP as substrate analogues.

# publications[].label
Cysteine Dealkylation in Bacillus subtilis by a Novel Flavin-Dependent Monooxygenase.

# publications[].label
U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems.

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Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation.

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Two-dimensional mass spectrometry of biomolecules at the subfemtomole level.

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Dibenzothiophene Catabolism Proceeds via a Flavin-N5-oxide Intermediate.

# publications[].label
BIOL 155-Mechanistic studies on pyridoxal phosphate synthase: The reaction pathway leading to a chromophoric intermediate

# publications[].label
Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5'-phosphate biosynthesis.

# publications[].label
Detection of four oxidation sites in viral prolyl-4-hydroxylase by top-down mass spectrometry.

# publications[].label
Structural studies on 3-hydroxyanthranilate-3,4-dioxygenase: the catalytic mechanism of a complex oxidation involved in NAD biosynthesis.

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Structural studies of thiamin monophosphate kinase in complex with substrates and products.

# publications[].label
Antibacterial Strategy against H. pylori: Inhibition of the Radical SAM Enzyme MqnE in Menaquinone Biosynthesis.

# publications[].label
Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae.

# publications[].label
The crystal structure and mechanism of orotidine 5'-monophosphate decarboxylase.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

# publications[].label
Cofactor biosynthesis: an organic chemist's treasure trove.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
NAD biosynthesis: identification of the tryptophan to quinolinate pathway in bacteria.

# publications[].label
The pyridine ring of NAD is formed by a nonenzymatic pericyclic reaction.

# publications[].label
Crystallization and preliminary X-ray analysis of thiaminase I from Bacillus thiaminolyticus: space group change upon freezing of crystals.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Phosphopantothenoylcysteine synthetase from Escherichia coli. Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria.

# publications[].label
RutA-Catalyzed Oxidative Cleavage of the Uracil Amide Involves Formation of a Flavin-N5-oxide.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .3. THE TRAPPING OF THE ONE-BOND-CLEAVED INTERMEDIATE FROM A PHOTODIMER RADICAL CATION MODEL SYSTEM

# publications[].label
Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis.

# publications[].label
Identification of modification sites in large biomolecules by stable isotope labeling and tandem high resolution mass spectrometry. The active site nucleophile of thiaminase I.

# publications[].label
Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.

# publications[].label
Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene.

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Menaquinone Biosynthesis: Biochemical and Structural Studies of Chorismate Dehydratase.

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Hexachlorobenzene Catabolism Involves a Nucleophilic Aromatic Substitution and Flavin-N5-Oxide Formation.

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Catalysis of a flavoenzyme-mediated amide hydrolysis.

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Mechanistic Studies on Prolyl-4-Hydroxylase: Demonstration That the Ferryl Intermediate Does Not Exchange with Water.

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Vitamin B1 de novo synthesis in the human malaria parasite Plasmodium falciparum depends on external provision of 4-amino-5-hydroxymethyl-2-methylpyrimidine.

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Tryptophan catabolism: identification and characterization of a new degradative pathway.

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Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily.

# publications[].label
Selective deprotection of tert-butyldimethylsilyl ether with lithium bromide and 18-crown-6

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PHENOXAZINONE SYNTHASE - ENZYMATIC CATALYSIS OF AN AMINOPHENOL OXIDATIVE CASCADE

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Aminofutalosine Synthase (MqnE): A New Catalytic Motif in Radical SAM Enzymology.

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Inhibitors of pantothenate kinase: novel antibiotics for staphylococcal infections.

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Mechanism-based inactivation of the human prolyl-4-hydroxylase by 5-oxaproline-containing peptides: Evidence for a prolyl radical intermediate

# publications[].label
The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase.

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Investigations into the Biosynthesis, Regulation, and Self-Resistance of Toxoflavin in Pseudomonas protegens Pf-5.

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Substrate analogues as mechanistic probes of methyl-S-coenzyme M reductase.

# publications[].label
Biosynthesis of Branched Alkoxy Groups: Iterative Methyl Group Alkylation by a Cobalamin-Dependent Radical SAM Enzyme.

# publications[].label
Tryptophan Lyase (NosL): A Cornucopia of 5'-Deoxyadenosyl Radical Mediated Transformations.

# publications[].label
Mechanistic Studies on the Single-Turnover Yeast Thiamin Pyrimidine Synthase: Characterization of the Inactive Enzyme.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .4. THE ENTHALPY OF CLEAVAGE OF A MODEL PHOTODIMER

# publications[].label
The selectivity for cysteine over serine in coenzyme A biosynthesis.

# publications[].label
Radical S-adenosylmethionine (SAM) enzymes in cofactor biosynthesis: a treasure trove of complex organic radical rearrangement reactions.

# publications[].label
The antibiotic activity of N-pentylpantothenamide results from its conversion to ethyldethia-coenzyme a, a coenzyme a antimetabolite.

# publications[].label
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.

# publications[].label
Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction.

# publications[].label
Top down characterization of secreted proteins from Mycobacterium tuberculosis by electron capture dissociation mass spectrometry.

# publications[].label
Thi20, a remarkable enzyme from Saccharomyces cerevisiae with dual thiamin biosynthetic and degradation activities.

# publications[].label
Anaerobic biosynthesis of the lower ligand of vitamin B12.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3 A resolution.

# publications[].label
Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.

# publications[].label
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.

# publications[].label
Mechanistic studies on thiamin formation and degradation: thiaminase I and thiamin phosphate synthase

# publications[].label
Structural insights into the mechanism of the PLP synthase holoenzyme from Thermotoga maritima.

# publications[].label
One enzyme for two radical reactions: Deciphering the mechanism of a novel radical SAM enzyme critical for F-420 cofactor biosynthesis

# publications[].label
Structure of a Clostridium botulinum C143S thiaminase I/thiamin complex reveals active site architecture .

# publications[].label
The vitamin B1 metabolism of Staphylococcus aureus is controlled at enzymatic and transcriptional levels.

# publications[].label
Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase.

# publications[].label
Identification of the product of toxoflavin lyase: degradation via a Baeyer-Villiger oxidation.

# publications[].label
Mechanistic studies on DNA photolyase, II. Is the blue enzyme isolated from Escherichia coli a mutant?

# publications[].label
An efficient enzymatic synthesis of thiamin pyrophosphate.

# publications[].label
Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation.

# publications[].label
Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamin biosynthesis in Bacillus subtilis.

# publications[].label
Comparison of femtosecond laser and continuous wave UV sources for protein-nucleic acid crosslinking.

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Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.

# publications[].label
Thiamin: A simple vitamin with a complex biosynthetic pathway

# publications[].label
Structural similarities between thiamin-binding protein and thiaminase-I suggest a common ancestor.

# publications[].label
The selectivity for cysteine over serine in coenzyme A biosynthesis (vol 6, pg 284, 2005)

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Competence of Thiamin Diphosphate-Dependent Enzymes with 2'-Methoxythiamin Diphosphate Derived from Bacimethrin, a Naturally Occurring Thiamin Anti-vitamin.

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Simultaneous kinetic characterization of multiple protein forms by top down mass spectrometry.

# publications[].label
PICOSECOND LASER PHOTOLYSIS STUDIES ON THE PHOTOREPAIR OF PYRIMIDINE DIMERS BY DNA PHOTOLYASE .1. LASER PHOTOLYSIS OF PHOTOLYASE-2-DEOXYURIDINE DINUCLEOTIDE PHOTODIMER COMPLEX

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Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5'-phosphate.

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DESIGN OF A CLEAVABLE LINKER FOR THE SYNTHESIS OF A CIS-SYN PYRIMIDINE PHOTODIMER

# publications[].label
Crystal structure of Mycobacterium tuberculosis polyketide synthase 11 (PKS11) reveals intermediates in the synthesis of methyl-branched alkylpyrones.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .1. SECONDARY DEUTERIUM-ISOTOPE EFFECTS ON THE CLEAVAGE OF 2'-DEOXYURIDINE DINUCLEOTIDE PHOTODIMERS

# publications[].label
Alternatives to vitamin B1 uptake revealed with discovery of riboswitches in multiple marine eukaryotic lineages.

# publications[].label
Catalysis of a new ribose carbon-insertion reaction by the molybdenum cofactor biosynthetic enzyme MoaA.

# publications[].label
Chemical biology: an educational challenge for chemistry departments.

# publications[].label
Mechanistic studies on thiaminase I. Overexpression and identification of the active site nucleophile.

# publications[].label
Menaquinone Biosynthesis: New Strategies to Trap Radical Intermediates in the MqnE-Catalyzed Reaction.

# publications[].label
The enzymology of sulfur activation during thiamin and biotin biosynthesis.

# publications[].label
Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis

# publications[].label
Biosynthesis of a thiamin antivitamin in Clostridium botulinum.

# publications[].label
Mechanistic studies on the repair of a novel DNA photolesion: the spore photoproduct.

# publications[].label
Phenoxazinone synthase: mechanism for the formation of the phenoxazinone chromophore of actinomycin.

# publications[].label
Biochemistry: Origin of a key player in methane biosynthesis.

# publications[].label
PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).

# publications[].label
Biosynthesis of F0, precursor of the F420 cofactor, requires a unique two radical-SAM domain enzyme and tyrosine as substrate.

# publications[].label
Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics.

# publications[].label
Aminofutalosine Synthase: Evidence for Captodative and Aryl Radical Intermediates Using -Scission and SRN1 Trapping Reactions.

# publications[].label
Stoichiometry and spectroscopic identity of copper centers in phenoxazinone synthase: a new addition to the blue copper oxidase family.

# publications[].label
Biosynthesis of thiamin thiazole: determination of the regiochemistry of the S/O acyl shift by using 1,4-dideoxy-D-xylulose-5-phosphate.

# publications[].label
Active-site models for complexes of quinolinate synthase with substrates and intermediates.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099.

# publications[].label
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.

# publications[].label
Construction of a thiamin sensor from the periplasmic thiamin binding protein.

# publications[].label
Formation of the chromophore of the pyoverdine siderophores by an oxidative cascade.

# publications[].label
Oxidative cascades: a facile biosynthetic strategy for the assembly of complex molecules.

# publications[].label
Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis.

# publications[].label
Crystal structure and mechanism of tryptophan 2,3-dioxygenase, a heme enzyme involved in tryptophan catabolism and in quinolinate biosynthesis.

# publications[].label
Molybdopterin biosynthesis: trapping an unusual purine ribose adduct in the MoaA-catalyzed reaction.

# publications[].label
Flavin-N5-oxide: A new, catalytic motif in flavoenzymology.

# publications[].label
Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry.

# publications[].label
Thiamin biosynthesis: still yielding fascinating biological chemistry.

# publications[].label
Glycal formation in crystals of uridine phosphorylase.

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .9. IS THE CLEAVAGE OF THE CYCLOBUTANE PYRIMIDINE PHOTODIMER RADICAL-ANION A PERICYCLIC REACTION

# publications[].label
Mechanistic studies on the biosynthesis and degradation of thiamin

# publications[].label
Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex.

# publications[].label
Thiamine deficiency in Cambodian infants with and without beriberi.

# publications[].label
Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster.

# publications[].label
A Remarkable Oxidative Cascade That Replaces the Riboflavin C8 Methyl with an Amino Group during Roseoflavin Biosynthesis.

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Fast, efficient localization of protein sequence error and covalent modification by high resolution tandem mass spectrometry.

# publications[].label
DNA-protein cross-linking: model systems for pyrimidine-aromatic amino acid cross-linking.

# publications[].label
Comprehensive Natural Products II Chemistry and Biology Overview and Introduction

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Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.

# publications[].label
PROTOCHLOROPHYLLIDE REDUCTASE .1. DETERMINATION OF THE REGIOCHEMISTRY AND THE STEREOCHEMISTRY OF THE REDUCTION OF PROTOCHLOROPHYLLIDE TO CHLOROPHYLLIDE

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Mechanistic studies on phosphopantothenoylcysteine decarboxylase: trapping of an enethiolate intermediate with a mechanism-based inactivating agent.

# publications[].label
PHENOXAZINONE SYNTHASE - ENZYMATIC CATALYSIS OF AN AMINOPHENOL OXIDATIVE CASCADE

# publications[].label
Thiaminase I (42 kDa) heterogeneity, sequence refinement, and active site location from high-resolution tandem mass spectrometry.

# publications[].label
NMR structure of the E-coli thiamine biosynthesis pathway protein ThiS

# publications[].label
Solid-state NMR and density functional theory studies of ionization states of thiamin.

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Mechanistic Studies on CysS - A Vitamin B12-Dependent Radical SAM Methyltransferase Involved in the Biosynthesis of the tert-Butyl Group of Cystobactamid.

# publications[].label
Sensitivity of breast cancer cell lines to recombinant thiaminase I.

# publications[].label
A novel mechanism of sulfur transfer catalyzed by O-acetylhomoserine sulfhydrylase in the methionine-biosynthetic pathway of Wolinella succinogenes.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
A genetic screen for the identification of thiamin metabolic genes.

# publications[].label
Structure of trifunctional THI20 from yeast.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
MECHANISTIC STUDIES ON DNA PHOTOLYASE .5. SECONDARY DEUTERIUM-ISOTOPE EFFECTS ON THE CLEAVAGE OF THE URACIL PHOTODIMER RADICAL CATION AND ANION

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Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate.

# publications[].label
Reversal of the substrate specificity of CMP N-glycosidase to dCMP.

# publications[].label
Spore photoproduct lyase from Bacillus subtilis spores is a novel iron-sulfur DNA repair enzyme which shares features with proteins such as class III anaerobic ribonucleotide reductases and pyruvate-formate lyases.

# publications[].label
The structural and biochemical foundations of thiamin biosynthesis.

# publications[].label
Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10.

# publications[].label
Toxoflavin lyase requires a novel 1-His-2-carboxylate facial triad.

# publications[].label
A maize thiamine auxotroph is defective in shoot meristem maintenance.

# publications[].label
Trapping and Electron Paramagnetic Resonance Characterization of the 5'dAdo Radical in a Radical S-Adenosyl Methionine Enzyme Reaction with a Non-Native Substrate.

# publications[].label
Structural characterization of the enzyme-substrate, enzyme-intermediate, and enzyme-product complexes of thiamin phosphate synthase.

# publications[].label
Alkylcysteine Sulfoxide C-S Monooxygenase Uses a Flavin-Dependent Pummerer Rearrangement.

# publications[].label
Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium tuberculosis.

# publications[].label
MECHANISTIC STUDIES ON PROTOCHLOROPHYLLIDE REDUCTASE - A MODEL SYSTEM FOR THE ENZYMATIC-REACTION

# publications[].label
Globally Important Haptophyte Algae Use Exogenous Pyrimidine Compounds More Efficiently than Thiamin.

# publications[].label
Cloning, sequencing and regulation of thiA, a thiamin biosynthesis gene from Bacillus subtilis.

# publications[].label
Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae.

# publications[].label
PHOTOENZYMES - A NOVEL CLASS OF BIOLOGICAL CATALYSTS

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Mechanistic studies of a protonolytic organomercurial cleaving enzyme: bacterial organomercurial lyase.

# publications[].label
Anaerobic 5-Hydroxybenzimidazole Formation from Aminoimidazole Ribotide: An Unanticipated Intersection of Thiamin and Vitamin B Biosynthesis.

# publications[].label
Crystal structure of thiaminase-I from Bacillus thiaminolyticus at 2.0 A resolution.

# publications[].label
Biochemical Characterization and Structural Basis of Reactivity and Regioselectivity Differences between Burkholderia thailandensis and Burkholderia glumae 1,6-Didesmethyltoxoflavin N-Methyltransferase.

# publications[].label
From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.

# publications[].label
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.

# publications[].label
A "radical dance" in thiamin biosynthesis: mechanistic analysis of the bacterial hydroxymethylpyrimidine phosphate synthase.

# publications[].label
Mechanistic studies on thiaminase I: Identification of the product of thiamin degradation in the absence of the nucleophilic cosubstrate

# publications[].label
Molybdopterin biosynthesis-Mechanistic studies on a novel MoaA catalyzed insertion of a purine carbon into the ribose of GTP.

# publications[].label
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: Identification of a novel acyl disulfide linked protein-protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

# publications[].label
Biosynthesis of the thiamin pyrimidine: the reconstitution of a remarkable rearrangement reaction.

# publications[].label
New cysteine biosynthetic pathway in mycobacterium tuberculosis.

# publications[].label
Reagent for the detection of protein thiocarboxylates in the bacterial proteome: lissamine rhodamine B sulfonyl azide.

# publications[].label
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.

# publications[].label
Solution structure of ThiS and implications for the evolutionary roots of ubiquitin.

# publications[].label
Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.

# publications[].label
The structural basis for the remarkable catalytic proficiency of orotidine 5'-monophosphate decarboxylase.

# publications[].label
Structural biology of enzymes of the thiamin biosynthesis pathway.

# publications[].label
Oxidative Dearomatization of PLP in Thiamin Pyrimidine Biosynthesis in Candida albicans.

# publications[].label
Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism.

# publications[].label
Menaquinone biosynthesis: formation of aminofutalosine requires a unique radical SAM enzyme.

# publications[].label
Structure of oxalate decarboxylase from Bacillus subtilis at 1.75 A resolution.

# publications[].label
Cofactor biosynthesis: A mechanistic perspective

# publications[].label
Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.

# publications[].label
Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.

# publications[].label
beta-scission of the N-O bond in alkyl hydroxamate radicals: a fast radical trap.

# publications[].label
Ligand-induced structural and dynamic changes in the C-Src SH3 domain

# publications[].label
Iterative Methylations Resulting in the Biosynthesis of the t-Butyl Group Catalyzed by a B12-Dependent Radical SAM Enzyme in Cystobactamid Biosynthesis.

# publications[].label
Biosynthesis of the thioquinolobactin siderophore: an interesting variation on sulfur transfer.

# publications[].label
13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase.

# publications[].label
Mechanistic Studies on Tryptophan Lyase (NosL): Identification of Cyanide as a Reaction Product.

# publications[].label
Pseudouridine monophosphate glycosidase: a new glycosidase mechanism.

# publications[].label
HMP binding protein ThiY and HMP-P synthase THI5 are structural homologues.

# publications[].label
Tryptophan catabolism: Gene identification and mechanistic studies

# publications[].label
Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate.

# publications[].label
Functional copper at the acetyl-CoA synthase active site.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Enzymatic reactions involving novel mechanisms of carbanion stabilization.

# publications[].label
The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS.

# publications[].label
Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution.

# publications[].label
A NEW SYNTHESIS OF 1-DEOXY-D-THREO-2-PENTULOSE, A BIOSYNTHETIC PRECURSOR TO THE THIAZOLE MOIETY OF THIAMIN

# publications[].label
Biosynthesis of dimethylbenzimidazole: Catalysis of a remarkable flavin degradation reaction

# publications[].label
O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.

# publications[].label
The mechanism of action of bacimethrin, a naturally occurring thiamin antimetabolite.

# publications[].label
Top down characterization of larger proteins (45 kDa) by electron capture dissociation mass spectrometry.

# publications[].label
A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.

# publications[].label
Tryptophan Lyase (NosL): Mechanistic Insights from Substrate Analogues and Mutagenesis.

# publications[].label
Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis.

# publications[].label
Structure of the PLP degradative enzyme 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti MAFF303099 and its mechanistic implications.

# publications[].label
The iron responsive sigma factor, AcsS, responsible for regulation of achromobactin biosynthesis in Pseudomonas syringae pv. syringae B728a

# publications[].label
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.

# publications[].label
The biosynthesis of coenzyme A in bacteria.

# publications[].label
Synthesis of P-32-labeled intermediates on the purine biosynthetic pathway

# publications[].label
Mechanistic Studies on Thiaminase I. 3. Stereochemistry of the Thiaminase I and the Bisulfite-Catalyzed Degradation of Chiral Monodeuteriothiamin.

# publications[].label
Thiamin phosphate synthase: the rate of pyrimidine carbocation formation.

# publications[].label
An assay for thiaminase I in complex biological samples.

# researcherOn[].label
Mentoring for the Future In Academic-Chemistry

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].label
New Radical SAM Enzymes Involved in Menaquinone biosynthesis

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Menaquinone Biosynthesis via the Futalosine Pathway

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
The Mechanistic Enzymology of Thiamin Biosynthesis

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Diabetes and Digestive and Kidney Disorders

# streetAddress
Texas A&M University; Chemistry Department; 3255 TAMU

# inTheNews[].label
The important role of the Young Scientist Exhibition

# inTheNews[].label
Antivitamins may soon replace antibiotics. Here's why

# inTheNews[].label
Are antivitamins the new antibiotics? Researchers develops drug approach against bacterial infections

# etdChairOf[].label
Chanani, Prem Kumar (2017-10). Novel Flavin Chemistry Involved in the Biosynthesis of the Lower Ligand of Vitamin B12. Doctoral Dissertation.

# etdChairOf[].label
Mehta, Angad P (2015-02). Mechanistic Studies on the Radical S-Adenosylmethionine Enzymes Involved in Molybdopterin, Thiamin and Vitamin B12 Biosynthesis. Doctoral Dissertation.

# etdChairOf[].label
Pyridoxal Phosphate as a Tag to  Identify Enzymes Within the "PLP-ome"

# etdChairOf[].label
Messer, Kayla J. (2012-07). Pyridoxal Phosphate as a Tag to  Identify Enzymes Within the "PLP-ome". Master's Thesis.

# etdChairOf[].label
Bhandari, Dhananjay (2017-09). Studies on the Flavin-mediated Cysteine Salvage Pathway, Structural Enzymology of Riboflavin Lyase and Mechanistic Investigation of the Radical S-adenosyl-L-methionine-mediated Tryptophan Lyase. Doctoral Dissertation.

# etdChairOf[].label
Wang, Yuanyou (2019-03). Mechanistic Studies of Radical SAM Enzymes in Cystobactamid, Anaerobilin and Thiamin Biosynthesis. Doctoral Dissertation.

# etdChairOf[].label
Chakrabarty, Yindrila (2016-08). Riboflavin Lyase: An Intriguing Flavoenzyme Involved in Riboflavin Degradation. Doctoral Dissertation.

# etdChairOf[].label
Mahanta, Nilkamal (2015-05). Studies on the Biosynthesis of Menaquinone (Vitamin K) and on the Catabolism of Pseudouridine. Doctoral Dissertation.

# etdChairOf[].label
Joshi, Sumedh Varad (2019-11). MECHANISTIC STUDIES ON RADICAL S-ADENOSYL-L-METHIONINE ENZYMES INVOLVED IN MENAQUINONE, THIAMIN AND COBALAMIN BIOSYNTHESIS. Doctoral Dissertation.

# etdChairOf[].label
Lai, Rung Yi (2015-05). The Mechanistic Investigation of the Bacterial Thiamin Thiazole Synthase and the Eukaryotic Thiamin Pyrimidine Synthase. Doctoral Dissertation.

# etdChairOf[].label
Jhulki, Isita (2020-01). Mechanistic Studies on a Remarkable Flavoenzyme Involved in Roseoflavin Biosynthesis. Doctoral Dissertation.

# etdChairOf[].label
Shome, Brateen (2017-01). Synthetic and Biochemical Exploration of the Degradation and Utilization of Thiamin Analogs and Preliminary Studies on Methanopterin methyltransferase. Doctoral Dissertation.

# etdChairOf[].label
Adak, Sanjoy (2019-11). Identification of New Catalytic Motifs in Flavoenzymes and Mechanistic Studies of a Cofactor Independent Oxygenase. Doctoral Dissertation.

# etdChairOf[].label
Liu, Yiquan (2015-10). Development of Chemical Strategies for Specifically Probing and Identifying Sulfur Carrier Proteins and Vitamin B6-Dependent Enzymes in Bacteria. Doctoral Dissertation.

# etdChairOf[].label
Xie, Lina (2018-08). Enzyme-Catalyzed Acylium Ion Formation and  Reagents for the Detection of Thiocarboxylates Involved in the  Biosynthesis of the NAD Derived Pincer Cofactor. Doctoral Dissertation.

# etdChairOf[].label
Olivard, Sarah (2012-11). Studies on Uptake of Thiamin Analogs by a Thiamin Deficient E. coli Mutant Strain. Master's Thesis.