# positions[].label
Director, Center for Craniofacial Research and Diagnosis

# positions[].label
Bernhard Gottlieb Endowed Chair for Craniofacial Research

# positions[].label
Professor and Head, Department of Periodontics

# overview
Stem Cells and Tissue Engineering In previous studies we have generated and characterized stem cell populations in dental tissues. We have also developed novel extracellular matrix-based scaffold materials. Currently we are performing a number of studies to examine the use of stem cells and scaffolds to regenerate periodontal and other tissues.

Chromatin, Epigenetics, and microRNAs Twenty years ago, we discovered the cp27 chromatin factor in our laboratory. This factor is part of the large SRCAP chromatin complex that plays important roles in development and cell division. A second aspect of our epigenetics research is focused on the role of histone methylation in odontogenic tissue differentiation and disease.

Periodontics Our lab works on the development and differentiation of periodontal tissues as a means to generate new progenitor based approaches for the regeneration of periodontal tissues. More recently, we have conducted studies to understand how epigenetic changes affect periodontal tissue response to pathogens.

Enamel Formation and Evolution Our lab is interested in determining the mechanisms of enamel crystal formation. We are asking how mineral ions are transported toward the enamel layer and what factors govern the nucleation and elongation of enamel crystals. Using an evolutionary biology approach, we are studying the relationship between the amelogenin molecule and enamel mechanical properties.

Evolution and Development Our lab focuses on the evolution of jaws and teeth, especially tooth enamel and periodontal ligament. Specifically, we are interested in the effects of changes in the amelogenin protein on the evolution of the amazing physical properties of enamel. We are also trying to understand how the non-mineralized state of the periodontal ligament evolved in vertebrates.

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Stem Cell Research - Nonembryonic - Human

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Stem Cell Research - Nonembryonic - Non-human

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
Intracellular Signaling Peptides And Proteins

# keywords[]
Intercellular Signaling Peptides And Proteins

# keywords[]
Insulin-like Growth Factor Binding Proteins

# educationAndTraining[].organization.label
Philipp University of Marburg - (Marburg, Germany)

# educationAndTraining[].organization.label
Philipp University of Marburg - (Marburg, Germany)

# publications[].label
BMP-2 regulates the formation of oral sulcus in mouse tongue by altering the balance between TIMP-1 and MMP-13.

# publications[].label
CP27 affects viability, proliferation, attachment and gene expression in embryonic fibroblasts.

# publications[].label
Effects of Exogenous Bone Morphogenetic Protein 2 on the Formation of Mouse Molar Tooth Germ

# publications[].label
Localization of microfilaments and microtubules during dental development in the rat.

# publications[].label
Evidence for amelogenin "nanospheres" as functional components of secretory-stage enamel matrix.

# publications[].label
Structure and function of ameloblastin as an extracellular matrix protein: adhesion, calcium binding, and CD63 interaction in human and mouse.

# publications[].label
Activation of -catenin signalling leads to temporomandibular joint defects.

# publications[].label
SM50 repeat-polypeptides self-assemble into discrete matrix subunits and promote appositional calcium carbonate crystal growth during sea urchin tooth biomineralization.

# publications[].label
Caiman periodontium as an intermediate between basal vertebrate ankylosis-type attachment and mammalian "true" periodontium.

# publications[].label
Immunohistochemical similarities and differences between amelogenin and tuftelin gene products during tooth development.

# publications[].label
The role of dust, grit and phytoliths in tooth wear

# publications[].publisher
Finnish Zoological and Botanical Publishing Board

# publications[].label
Successful Application of a Galanin-Coated Scaffold for Periodontal Regeneration.

# publications[].label
Discussion: Session 3. The inorganic phase: 2

# publications[].label
Evolution in tooth developmental biology: of morphology and molecules.

# publications[].label
Pseudogenized Amelogenin Reveals Early Tooth Loss in True Toads (Anura: Bufonidae).

# publications[].label
Chapter 35 Morphogenesis and Wound Healing in the Periodontium

# publications[].label
Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization.

# publications[].label
An in vitro study of various instruments for root planing

# publications[].label
Characterization of the mouse CP27 promoter and NF-Y mediated gene regulation.

# publications[].label
Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2.

# publications[].label
Membranes, minerals, and proteins of developing vertebrate enamel.

# publications[].label
Novel approaches toward managing the micromanagers: 'non-toxic' but effective.

# publications[].label
NF-Y and USF1 transcription factor binding to CCAAT-box and E-box elements activates the CP27 promoter.

# publications[].publisher
The International Journal of Developmental Biology

# publications[].label
Response of periradicular tissues to growth factors introduced into the surgical site in the root-end filling material.

# publications[].label
Expression and characterization of a Rana pipiens amelogenin protein.

# publications[].label
Pattern formation in development of chondrichthyan dentitions: a review of an evolutionary model

# publications[].label
Abnormal Ras signaling in Costello syndrome (CS) negatively regulates enamel formation.

# publications[].label
Propagation of Dental and Respiratory Cells and Organs in Microgravity.

# publications[].label
CP27 localization in the dental lamina basement membrane and in the stellate reticulum of developing teeth.

# publications[].label
The ameloblastin extracellular matrix molecule enhances bone fracture resistance and promotes rapid bone fracture healing.

# publications[].label
Varanoid Tooth Eruption and Implantation Modes in a Late Cretaceous Mosasaur.

# publications[].label
Polarized, Amelogenin Expressing Ameloblast-Like Cells from Cervical Loop/Dental Pulp Cocultures in Bioreactors.

# publications[].label
The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex.

# publications[].label
Amelogenin sequence and enamel biomineralization in Rana pipiens.

# publications[].label
Daughters of the Enamel Organ: Development, Fate, and Function of the Stratum Intermedium, Stellate Reticulum, and Outer Enamel Epithelium.

# publications[].label
Cloning, gene expression, and characterization of CP27, a novel gene in mouse embryogenesis.

# publications[].label
Osteopontin is required for unloading-induced osteoclast recruitment and modulation of RANKL expression during tooth drift-associated bone remodeling, but not for super-eruption.

# publications[].label
A review of the aggregation properties of a recombinant amelogenin.

# publications[].label
Changes in Hox Gene Chromatin Organization during Odontogenic Lineage Specification.

# publications[].label
Initial enamel crystals are not spatially associated with mineralized dentine.

# publications[].label
Evolution: Herbivore-Type Teeth in a Cretaceous Tuatara Relative.

# publications[].label
Amelogenesis: Transformation of a protein-mineral matrix into tooth enamel.

# publications[].label
[Immunohistochemical detections of intermediary filaments in periodontal structures and other soft tissue in oral cavity].

# publications[].label
True enamel covering in teeth of the Australian lungfish Neoceratodus forsteri.

# publications[].label
Identification and characterization of a squamate reptilian amelogenin gene: Iguana iguana.

# publications[].label
The use of light/chemically hardened polymethylmethacrylate, polyhydroxylethylmethacrylate, and calcium hydroxide graft material in combination with polyanhydride around implants and extraction sockets in minipigs: Part II: histologic and micro-CT evaluations.

# publications[].label
Conservation and variation in enamel protein distribution during vertebrate tooth development.

# publications[].label
Posttranslational Amelogenin Processing and Changes in Matrix Assembly during Enamel Development.

# publications[].label
Intravesicular Phosphatase PHOSPHO1 Function in Enamel Mineralization and Prism Formation.

# publications[].label
Gene expression, signal transduction and tissue-specific biomineralization during mammalian tooth development.

# publications[].label
Extracellular matrix-mediated tissue remodeling following axial movement of teeth.

# publications[].label
The use of light/chemically hardened polymethylmethacrylate, polyhydroxyethylmethacrylate, and calcium hydroxide graft material in combination with polyanhydride around implants in minipigs: part I: immediate stability and function.

# publications[].label
Pathways and fate of migratory cells during late tooth organogenesis.

# publications[].label
An in vitro study of various instruments for root planing.

# publications[].label
-catenin Initiates Tooth Neogenesis in Adult Rodent Incisors

# publications[].label
Expression and function of enamel-related gene products in calvarial development.

# publications[].label
Dental follicle progenitor cell heterogeneity in the developing mouse periodontium.

# publications[].label
The Glycoprotein/Cytokine Erythropoietin Promotes Rapid Alveolar Ridge Regeneration In Vivo by Promoting New Bone Extracellular Matrix Deposition in Conjunction with Coupled Angiogenesis/Osteogenesis.

# publications[].label
Subunit compartments of secretory stage enamel matrix.

# publications[].label
Exogenous hepatocyte growth factor inhibits myoblast differentiation by inducing myf5 expression and suppressing myoD expression in an organ culture system of embryonic mouse tongue.

# publications[].label
Our periodontal tissue: a masterpiece of evolution.

# publications[].label
Successful periodontal ligament regeneration by periodontal progenitor preseeding on natural tooth root surfaces.

# publications[].label
Tissue pressure and YAP during organogenesis.

# publications[].label
Integrative Temporo-Spatial, Mineralogic, Spectroscopic, and Proteomic Analysis of Postnatal Enamel Development in Teeth with Limited Growth.

# publications[].label
Meeting report: a hard look at the state of enamel research.

# publications[].label
When genomes and stem cells meet: New perspectives for medical applications of stem cell research through the Australian/U.S. collaborative.

# publications[].label
RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model.

# publications[].label
Alternative Splicing of the Amelogenin Gene in a Caudate Amphibian, Plethodon cinereus.

# publications[].label
Morphogenesis and Wound Healing in the Periodontium

# publications[].label
MicroRNAs: Harbingers and shapers of periodontal inflammation.

# publications[].label
CP27 function is necessary for cell survival and differentiation during tooth morphogenesis in organ culture.

# publications[].label
Bones, teeth, and genes: a genomic homage to Harry Sicher's "Axial Movement of Teeth".

# publications[].label
Discussion: Session 1. Ameloblast cell biology

# publications[].label
Triple PLGA/PCL Scaffold Modification Including Silver Impregnation, Collagen Coating, and Electrospinning Significantly Improve Biocompatibility, Antimicrobial, and Osteogenic Properties for Orofacial Tissue Regeneration.

# publications[].label
Function of enamel gene products in other tissues - Discussio of session 6

# publications[].label
Enamel biomimetics-fiction or future of dentistry.

# publications[].label
Ameloblastin-rich enamel matrix favors short and randomly oriented apatite crystals.

# publications[].label
The Wnt Antagonist SFRP1: A Key Regulator of Periodontal Mineral Homeostasis.

# publications[].label
prx-1 functions cooperatively with another paired-related homeobox gene, prx-2, to maintain cell fates within the craniofacial mesenchyme.

# publications[].label
The expanded amelogenin polyproline region preferentially binds to apatite versus carbonate and promotes apatite crystal elongation.

# publications[].label
BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue.

# publications[].label
Discussion: Session 4. Enamel mineralization

# publications[].label
Epigenetic marks define the lineage and differentiation potential of two distinct neural crest-derived intermediate odontogenic progenitor populations.

# publications[].label
Session 3. The inorganic phase: 2 - Discussion

# publications[].label
Elongated polyproline motifs facilitate enamel evolution through matrix subunit compaction.

# publications[].label
Session 1. Ameloblast cell biology - Discussion

# publications[].label
Roles of insulin-like growth factors and their binding proteins in the differentiation of mouse tongue myoblasts.

# publications[].label
Development and histochemistry of vertebrate teeth - Introduction

# publications[].label
Antisense inhibition of AMEL translation demonstrates supramolecular controls for enamel HAP crystal growth during embryonic mouse molar development.

# publications[].label
CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation.

# publications[].label
Amelogenin supramolecular assembly in nanospheres defined by a complex helix-coil-PPII helix 3D-structure.

# publications[].label
Periodontal Homeostasis: From Vienna to Texas-A Century of Periodontal Research in the Spirit of Bernhard Gottlieb.

# publications[].label
Amelogenin evolution and tetrapod enamel structure.

# publications[].label
Cyclic stretch and compression forces alter microRNA-29 expression of human periodontal ligament cells.

# publications[].label
Titanium particles generated during ultrasonic scaling of implants.

# publications[].label
Change from a hard to soft diet alters the expression of insulin-like growth factors, their receptors, and binding proteins in association with atrophy in adult mouse masseter muscle.

# publications[].label
Combinatorial Design of Hydrolytically Degradable, Bone-like Biocomposites Based on PHEMA and Hydroxyapatite.

# publications[].label
Long-acting PFI-2 small molecule release and multilayer scaffold design achieve extensive new formation of complex periodontal tissues with unprecedented fidelity.

# publications[].label
Apatite microtopographies instruct signaling tapestries for progenitor-driven new attachment of teeth.

# publications[].label
Timing of the expression of enamel gene products during mouse tooth development.

# publications[].label
SETD1 and NF-B Regulate Periodontal Inflammation through H3K4 Trimethylation.

# publications[].label
Histone Methylation: Achilles Heel and Powerful Mediator of Periodontal Homeostasis.

# publications[].label
Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damage in mdx mice.

# publications[].label
Sequential distribution of keratan sulphate and chondroitin sulphate epitopes during ameloblast differentiation.

# publications[].label
Enamel Research: Priorities and Future Directions.

# publications[].label
Vienna-Chicago: the cultural transformation of the model system of the un-opposed molar.

# publications[].label
Self-assembly of a recombinant amelogenin protein generates supramolecular structures.

# publications[].label
Epigenetic Repression of RUNX2 and OSX Promoters Controls the Nonmineralized State of the Periodontal Ligament.

# publications[].label
A three dimensional free body analysis describing variation in the musculoskeletal configuration of the cynodont lower jaw.

# publications[].label
Molecular strategies of tooth enamel formation are highly conserved during vertebrate evolution.

# publications[].label
MicroRNAs and immunity in periodontal health and disease.

# publications[].label
Proteolysis and evolutionary aspects in enamel formation - Discussion of session 2

# publications[].label
Evolution and development of Hertwig's epithelial root sheath.

# publications[].label
Microporous Bio-orthogonally Annealed Particle Hydrogels for Tissue Engineering and Regenerative Medicine.

# publications[].label
Case study: polycystic livers in a transgenic mouse line.

# publications[].label
Differentiation of neural-crest-derived intermediate pluripotent progenitors into committed periodontal populations involves unique molecular signature changes, cohort shifts, and epigenetic modifications.

# researcherOn[].label
Ameloblast Differentiation and Amelogenesis:  Next-Generation Models to Define Key Mechanisms and Factors Involved in Biological Enamel Formation

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Dental and Craniofacial Research

# researcherOn[].label
Neurobiological Control of Periodontal Homeostasis through MicroRNA, TGF-beta, and Wnt Signaling

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Dental and Craniofacial Research

# researcherOn[].label
NIDCR R01 DE026198 â€œSmall molecule mediated restoration of periodontal homeostasis through the YAP1 pathway

# researcherOn[].awardedBy.label
National Institute of Dental and Craniofacial Research - (Bethesda, Maryland, United States)

# researcherOn[].label
3R01DE018900-01A1S1 ARRA Stimulus Supplement for DE018900

# researcherOn[].awardedBy.label
National Institute of Dental and Craniofacial Research - (Bethesda, Maryland, United States)

# researcherOn[].label
NIDCR R01 DE018900 â€œEnamel Structure Sophistication through Amelogenin Evolutionâ€; additional R01 and R03 grants to fellow lab members

# researcherOn[].awardedBy.label
National Institute of Dental and Craniofacial Research - (Bethesda, Maryland, United States)

# streetAddress
Texas A&M University College of Dentistry; Periodontics; 3302 Gaston Ave

# etdChairOf[].label
Ponsford, Mitchell Wagner (2018-04). Foreign Bodies in Proximity to Failing Dental Implants. Master's Thesis.