# overview
A long-term research goal of our group is to determine the molecular basis behind protein-lipid interactions and how these interactions can modulate the structure and function of membrane proteins, including their interactions with signaling molecules. What determines the selectivity of membrane proteins towards lipids, and the coupling between lipid binding events and function remains a key knowledge gap in the field; one that if addressed will significantly advance our understanding of how lipids participate in both normal and pathophysiological processes of membrane proteins. Therefore, there is a critical need to expand our fundamental knowledge in this emerging field by applying and developing innovative approaches to elucidate how lipids modulate the structure function of membrane proteins. To this end, we are studying a number of ion channels, receptors and other types of membrane proteins.

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Mass Spectrometry Of Intact Membrane Protein Complexes

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
Periodic Focusing Drift Tube Ion Mobility

# educationAndTraining[].organization.label
California State University, Chico - (Chico, California, United States)

# educationAndTraining[].organization.label
California State University, Chico - (Chico, California, United States)

# educationAndTraining[].organization.label
University of California Los Angeles - (Los Angeles, California, United States)

# publications[].label
Variable-Temperature ESI-IMS-MS Analysis of Myohemerythrin Reveals Ligand Losses, Unfolding, and a Non-Native Disulfide Bond.

# publications[].label
First-Principles Collision Cross Section Measurements of Large Proteins and Protein Complexes.

# publications[].label
An approach to crystallizing proteins by metal-mediated synthetic symmetrization.

# publications[].label
Sequencing covalent modifications of membrane proteins.

# publications[].label
C-terminal interactions mediate the quaternary dynamics of B-crystallin.

# publications[].label
Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the Stabilitome.

# publications[].label
Melting Proteins: Evidence for Multiple Stable Structures upon Thermal Denaturation of Native Ubiquitin from Ion Mobility Spectrometry-Mass Spectrometry Measurements.

# publications[].label
Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion.

# publications[].label
Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry.

# publications[].label
Atomic view of a toxic amyloid small oligomer.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions

# publications[].label
FTflow: An Open-Source Python GUI for FT-IM-MS Experiments.

# publications[].label
The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase.

# publications[].label
Capturing RAS oligomerization on a membrane.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Combining native mass spectrometry and lipidomics to uncover specific membrane protein-lipid interactions from natural lipid sources.

# publications[].label
Surface induced dissociation as a tool to study membrane protein complexes.

# publications[].label
A novel 3-methylhistidine modification of yeast ribosomal protein Rpl3 is dependent upon the YIL110W methyltransferase.

# publications[].label
Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology.

# publications[].label
Nonlinear frequency modulation for Fourier transform ion mobility mass spectrometry improves experimental efficiency

# publications[].label
Water Plays Key Roles in Stabilities of Wild Type and Mutant Transthyretin Complexes.

# publications[].label
Cardiolipin Regulates the Activity of the Mitochondrial ABC Transporter ABCB10.

# publications[].label
TREK2 Lipid Binding Preferences Revealed by Native Mass Spectrometry.

# publications[].label
Native Mass Spectrometry of Membrane Protein-Lipid Interactions in Different Detergent Environments.

# publications[].label
Variable-Temperature Electrospray Ionization for Temperature-Dependent Folding/Refolding Reactions of Proteins and Ligand Binding.

# publications[].label
Origin of complexity in haemoglobin evolution.

# publications[].label
Real time characterization of the MAPK pathway using native mass spectrometry.

# publications[].label
Molecular basis for amyloid-beta polymorphism.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Crystal structures of truncated alphaA and alphaB crystallins reveal structural mechanisms of polydispersity important for eye lens function.

# publications[].label
Mass spectrometry of intact membrane protein complexes.

# publications[].label
Polyamine detergents tailored for native mass spectrometry studies of membrane proteins.

# publications[].label
Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion.

# publications[].label
Structural basis for lipid and copper regulation of the ABC transporter MsbA.

# publications[].label
Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer.

# publications[].label
Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions.

# publications[].label
Selective regulation of human TRAAK channels by biologically active phospholipids.

# publications[].label
Topological Analysis of Transthyretin Disassembly Mechanism: Surface-Induced Dissociation Reveals Hidden Reaction Pathways.

# publications[].label
Structural basis of ion transport and inhibition in ferroportin.

# publications[].label
Step toward Probing the Nonannular Belt of Membrane Proteins.

# publications[].label
Allostery revealed within lipid binding events to membrane proteins.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Chapter 1 An Introduction to the Basic Principles and Concepts of Mass Spectrometry

# publications[].label
Molecular Basis for the Activation of Pseudomonas aeruginosa MsbA by Divalent Metals.

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Development of Native MS Capabilities on an Extended Mass Range Q-TOF MS.

# publications[].label
Determining Membrane Protein-Lipid Binding Thermodynamics Using Native Mass Spectrometry.

# publications[].label
Structure of a designed protein cage that self-assembles into a highly porous cube.

# publications[].label
Self-Masked Aldehyde Inhibitors: A Novel Strategy for Inhibiting Cysteine Proteases.

# publications[].label
A hydrophobic ratchet entrenches molecular complexes.

# publications[].label
Hydroponics on a chip: analysis of the Fe deficient Arabidopsis thylakoid membrane proteome.

# publications[].label
Effects of Hydration on Transthyretin Conformational Dynamics and Oligomerization.

# publications[].label
Generation of Charge-Reduced Ions of Membrane Protein Complexes for Native Ion Mobility Mass Spectrometry Studies.

# publications[].label
Collision-Induced Unfolding Studies of Proteins and Protein Complexes using Drift Tube Ion Mobility-Mass Spectrometer.

# publications[].label
Mechanism of anion exchange and small-molecule inhibition of pendrin.

# publications[].label
Protonation state of glutamate 73 regulates the formation of a specific dimeric association of mVDAC1.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Author Correction: Origin of complexity in haemoglobin evolution.

# publications[].label
Mass spectral analyses of the two major apolipoproteins of great ape high density lipoproteins.

# publications[].label
Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8.

# publications[].label
Evidence for Many Unique Solution Structures for Chymotrypsin Inhibitor 2: A Thermodynamic Perspective Derived from vT-ESI-IMS-MS Measurements.

# publications[].label
Roles of Hydration in Protein-Ligand Binding: Passive or Active Participant?

# publications[].label
2'-Deoxy Guanosine Nucleotides Alter the Biochemical Properties of Ras.

# publications[].label
Protons Are Fast and Smart; Proteins Are Slow and Dumb: On the Relationship of Electrospray Ionization Charge States and Conformations.

# publications[].label
Native mass spectrometry of proteoliposomes containing integral and peripheral membrane proteins.

# publications[].label
A Sec14-like phosphatidylinositol transfer protein paralog defines a novel class of heme-binding proteins.

# publications[].label
Detergent-free mass spectrometry of membrane protein complexes.

# publications[].label
Characterization of Membrane Protein-Lipid Interactions by Mass Spectrometry Ion Mobility Mass Spectrometry.

# publications[].label
Insight into the Phospholipid-Binding Preferences of Kir3.4.

# publications[].label
Membrane proteins bind lipids selectively to modulate their structure and function.

# publications[].label
Structural Analysis of the Effect of a Dual-FLAG Tag on Transthyretin.

# publications[].label
Entropy in the Molecular Recognition of Membrane Protein-Lipid Interactions.

# publications[].label
Quantifying the stabilizing effects of protein-ligand interactions in the gas phase.

# publications[].label
Adapting a Trapped Ion Mobility Spectrometry-Q-TOF for High m/z Native Mass Spectrometry and Surface-Induced Dissociation.

# publications[].label
Dissecting the Thermodynamics of ATP Binding to GroEL One Nucleotide at a Time

# publications[].label
Dissecting the Thermodynamics of ATP Binding to GroEL One Nucleotide at a Time.

# publications[].label
High-Throughput Mass Spectrometry for Biopharma: A Universal Modality and Target Independent Analytical Method for Accurate Biomolecule Characterization.

# publications[].label
Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
New insights into the metal-induced oxidative degradation pathways of transthyretin.

# publications[].label
Intrinsic GTPase Activity of K-RAS Monitored by Native Mass Spectrometry.

# publications[].label
Allostery without Conformational Change: A Native Mass Spectrometry Perspective.

# publications[].label
Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry.

# publications[].label
Structural basis for lipid and copper regulation of the ABC transporter MsbA

# publications[].label
Surface Activity of Amines Provides Evidence for the Combined ESI Mechanism of Charge Reduction for Protein Complexes.

# publications[].label
Ag+ Ion Binding to Human Metallothionein-2A Is Cooperative and Domain Specific.

# publications[].label
Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab.

# publications[].label
Covalent Inactivation of Mycobacterium tuberculosis Isocitrate Lyase by cis-2,3-Epoxy-Succinic Acid.

# publications[].label
Dynamical coupling of intrinsically disordered proteins and their hydration water: comparison with folded soluble and membrane proteins.

# publications[].label
Selective binding of a toxin and phosphatidylinositides to a mammalian potassium channel.

# publications[].label
Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry.

# publications[].label
Design and Synthesis of Hetero-Bicephalic Detergents for Native Mass Spectrometry of Membrane Proteins.

# publications[].label
Allosteric modulation of protein-protein interactions by individual lipid binding events.

# publications[].label
Catalytic Mechanism of SARS-CoV2 3Chymotrypsin-Like Protease as Determined by Steady-State and Pre-Steady-State Kinetics

# publications[].label
Hydration (H2O and D2O) Dictates the Stabilities and Conformational Entropy of Transthyretin Tetramers.

# publications[].label
A conserved PLPLRT/SD motif of STING mediates the recruitment and activation of TBK1.

# publications[].label
Insight into the Selectivity of Kir3.2 toward Phosphatidylinositides.

# publications[].label
Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry.

# publications[].label
Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer.

# publications[].label
The structured core domain of B-crystallin can prevent amyloid fibrillation and associated toxicity.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Melting of Hemoglobin in Native Solutions as measured by IMS-MS.

# publications[].label
The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry.

# publications[].label
Native IM-Orbitrap MS: Resolving What Was Hidden.

# publications[].label
Charge Engineering Reveals the Roles of Ionizable Side Chains in Electrospray Ionization Mass Spectrometry.

# publications[].label
Non-3D domain swapped crystal structure of truncated zebrafish alphaA crystallin.

# publications[].label
Variable-Temperature Native Mass Spectrometry for Studies of Protein Folding, Stabilities, Assembly, and Molecular Interactions.

# publications[].label
Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies.

# publications[].label
Native mass spectrometry and structural studies reveal modulation of MsbA-nucleotide interactions by lipids.

# publications[].label
Variation of CI-2 Conformers upon Addition of Methanol to Water: An IMS-MS-Based Thermodynamic Analysis.

# publications[].label
Temperature Regulates Stability, Ligand Binding (Mg2+ and ATP), and Stoichiometry of GroEL-GroES Complexes.

# publications[].label
Entropy in the molecular recognition of membrane protein-lipid interactions

# publications[].label
Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions.

# publications[].label
Imperfect symmetry facilitated the evolution of specificity and high-order stoichiometry in vertebrate hemoglobin.

# publications[].label
Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants.

# publications[].publisher
Proceedings of the National Academy of Sciences

# researcherOn[].label
Native ion mobility mass spectrometry studies of potassium inward rectifier channels: insight into gating and lipid binding

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# researcherOn[].label
Elucidating the stoichiometry of GPCR oligomers

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].label
Innovative Native Ion Mobility Approaches for Transformational Measurements in Structural Biology

# researcherOn[].awardedBy.label
National Institute of General Medical Sciences - (Bethesda, Maryland, United States)

# researcherOn[].label
Analysis of Staphylococcus Aureus Host Interactions

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
Elucidating the stoichiometry of GPCR oligomers

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Neurological Disorders and Stroke

# researcherOn[].label
Native ion mobility mass spectrometry studies of potassium inward rectifier channels: insight into gating and lipid binding

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# streetAddress
Texas A&M University; Chemistry; 3255 TAMU

# inTheNews[].label
A Surprisingly Simple Biochemistry Rule Drives the Evolution of Useless Complexity