# overview
We are developing and applying Magnetic resonance techniques for the investigation of rapid processes and molecular dynamics. Hyperpolarization of nuclear spins yields unprecedented levels of signal, which enables us to acquire NMR spectra of reactions as they occur, in real time. Applications of these techniques include the fields of enzyme catalysis, reactions in organic chemistry, polymers, and more.

To enable the use of hyperpolarization in NMR, we develop new hardware and specially adapted NMR experiments, and investigate the dynamics of hyperpolarized spin systems.

Hand-in-hand with hyperpolarization, we use modern multi-dimensional NMR for the investigation of basic determinants of protein structure and function, including of membrane proteins.

# keywords[]
0306 Physical Chemistry (incl. Structural)

# keywords[]
0306 Physical Chemistry (incl. Structural)

# keywords[]
0202 Atomic, Molecular, Nuclear, Particle And Plasma Physics

# keywords[]
0303 Macromolecular And Materials Chemistry

# keywords[]
0306 Physical Chemistry (incl. Structural)

# keywords[]
0306 Physical Chemistry (incl. Structural)

# keywords[]
0306 Physical Chemistry (incl. Structural)

# keywords[]
4012 Fluid Mechanics And Thermal Engineering

# keywords[]
3403 Macromolecular And Materials Chemistry

# keywords[]
Shaker Superfamily Of Potassium Channels

# keywords[]
4.1 Discovery And Preclinical Testing Of Markers And Technologies

# educationAndTraining[].organization.label
Swiss Federal Institute of Technology in Zurich - (Zurich, Switzerland)

# educationAndTraining[].organization.label
Swiss Federal Institute of Technology in Zurich - (Zurich, Switzerland)

# awardsAndHonors[].label
Association of Former Students College-Level Teaching Distinguished Achievement Award, The

# awardsAndHonors[].label
Faculty Early Career Development (CAREER) Program

# publications[].label
Chemical shift correlations from hyperpolarized NMR using a single SHOT.

# publications[].label
Nuclear magnetic resonance of hyperpolarized fluorine for characterization of protein-ligand interactions.

# publications[].label
Applications of Dissolution-DNP for NMR Screening.

# publications[].label
Membrane protein dynamics in different environments: simulation study of the outer membrane protein X in a lipid bilayer and in a micelle.

# publications[].label
CHED 1468-Assessment of reforms in the physical chemistry laboratory at Texas A&M University

# publications[].label
Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.

# publications[].label
Effective rotational correlation times of proteins from NMR relaxation interference.

# publications[].label
Temporal chemical shift correlations in reactions studied by hyperpolarized nuclear magnetic resonance.

# publications[].label
Biomolecular interactions studied by low-field NMR using SABRE hyperpolarization

# publications[].label
Reversal of the hofmeister series: specific ion effects on peptides.

# publications[].label
NMR analysis on microfluidic devices by remote detection.

# publications[].label
Facilitated substrate transport through membrane proteins.

# publications[].label
Early time kinetics and mechanisms of polymerization reactions by hyperpolarized NMR

# publications[].label
Spin coherence transfer in chemical transformations monitored by remote detection NMR.

# publications[].label
Bilayer in small bicelles revealed by lipid-protein interactions using NMR spectroscopy.

# publications[].label
Determination of Intermolecular Interactions Using Polarization Compensated Heteronuclear Overhauser Effect of Hyperpolarized Spins.

# publications[].label
Direct observation of Ru-alkylidene forming into ethylene in ring-closing metathesis from hyperpolarized 1 H NMR

# publications[].label
Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands.

# publications[].label
Method for accurate measurements of nuclear-spin optical rotation for applications in correlated optical-NMR spectroscopy.

# publications[].label
An NMR Experiment Based on Off-the-Shelf Digital Data-Acquisition Equipment

# publications[].label
Methanol strengthens hydrogen bonds and weakens hydrophobic interactions in proteins--a combined molecular dynamics and NMR study.

# publications[].label
Effects of End Group Termination on Salting-Out Constants for Triglycine.

# publications[].label
Ultrafast multidimensional Laplace NMR for a rapid and sensitive chemical analysis.

# publications[].label
Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase.

# publications[].label
Lipid-protein interactions in DHPC micelles containing the integral membrane protein OmpX investigated by NMR spectroscopy.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Applications of dynamic nuclear polarization to the study of reactions and reagents in organic and biomolecular chemistry.

# publications[].label
Parallelized Ligand Screening Using Dissolution Dynamic Nuclear Polarization.

# publications[].label
Nonrandom structure in the urea-unfolded Escherichia coli outer membrane protein X (OmpX).

# publications[].label
Metallocene-catalyzed olefin polymerization studied by dissolution dynamic nuclear polarization (DNP) NMR

# publications[].label
Dispersion measurements using time-of-flight remote detection MRI.

# publications[].label
Determination of binding affinities using hyperpolarized NMR with simultaneous 4-channel detection.

# publications[].label
Polarization Transfer from Ligands Hyperpolarized by Dissolution Dynamic Nuclear Polarization for Screening in Drug Discovery.

# publications[].label
Folding of a tryptophan zipper peptide investigated on the basis of the nuclear Overhauser effect and thermal denaturation.

# publications[].label
Microfluidic gas-flow profiling using remote-detection NMR.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Detection of Protein-Ligand Interactions by 19F Nuclear Magnetic Resonance Using Hyperpolarized Water.

# publications[].label
In Situ Determination of Tacticity, Deactivation, and Kinetics in [rac-(C2H4(1-Indenyl)2)ZrMe][B(C6F5)4] and [Cp2ZrMe][B(C6F5)4]-Catalyzed Polymerization of 1-Hexene Using (13)C Hyperpolarized NMR.

# publications[].label
Sequentially acquired two-dimensional NMR spectra from hyperpolarized sample.

# publications[].label
NMR-solution structures in methanol of an alpha-heptapeptide, of a beta(3)/beta(2)-nonapeptide, and of an all-beta(3)-icosapeptide carrying the 20 proteinogenic side chains

# publications[].label
Hyperpolarized Hadamard spectroscopy using flow NMR.

# publications[].label
Real-Time Analysis of Folding upon Binding of a Disordered Protein by Using Dissolution DNPNMR Spectroscopy.

# publications[].label
Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant.

# publications[].label
Spectrally resolved magnetic resonance imaging of a xenon biosensor.

# publications[].label
An ultra-low cost NMR device with arbitrary pulse programming.

# publications[].label
NMR structure of the integral membrane protein OmpX.

# publications[].label
Time-of-flight flow imaging of two-component flow inside a microfluidic chip.

# publications[].label
NMR-structural investigations of a beta(3)-dodecapeptide with proteinogenic side chains in methanol and in aqueous solutions

# publications[].label
Molecular mechanisms of ion-specific effects on proteins.

# publications[].label
Indirect detection of intermediate in decarboxylation reaction of phenylglyoxylic acid by hyperpolarized 13C NMR.

# publications[].label
PHYS 241-Time-resolved NMR by dynamic nuclear polarization

# publications[].label
Side chain NMR assignments in the membrane protein OmpX reconstituted in DHPC micelles.

# publications[].label
Protein folding studied by dissolution dynamic nuclear polarization.

# publications[].label
Hyperpolarized water as universal sensitivity booster in biomolecular NMR.

# publications[].label
Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents.

# publications[].label
Solution NMR of polypeptides hyperpolarized by dynamic nuclear polarization.

# publications[].label
Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles.

# publications[].label
Time-resolved dynamic nuclear polarization enhanced NMR spectroscopy.

# publications[].label
An NH moiety is not required for anion binding to amides in aqueous solution.

# publications[].label
Folding determinants of disulfide bond forming protein B explored by solution nuclear magnetic resonance spectroscopy.

# publications[].label
Chemical shift correlations from hyperpolarized NMR by off-resonance decoupling.

# publications[].label
Assessment of reforms in the physical chemistry laboratory

# publications[].label
Time- and site-resolved kinetic NMR for real-time monitoring of off-equilibrium reactions by 2D spectrotemporal correlations.

# publications[].label
2D NMR spectroscopy of refolding RNase Sa using polarization transfer from hyperpolarized water.

# publications[].label
Polyolefin catalysis of propene, 1-butene and isobutene monitored using hyperpolarized NMR.

# publications[].label
Amplification of Nuclear Overhauser Effect Signals by Hyperpolarization for Screening of Ligand Binding to Immobilized Target Proteins.

# publications[].label
Rapid sample injection for hyperpolarized NMR spectroscopy.

# publications[].label
Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli.

# publications[].label
Metabolic Measurements of Nonpermeating Compounds in Live Cells Using Hyperpolarized NMR

# publications[].label
Reaction monitoring using hyperpolarized NMR with scaling of heteronuclear couplings by optimal tracking.

# publications[].label
Molecular imaging using a targeted magnetic resonance hyperpolarized biosensor.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Detection of living anionic species in polymerization reactions using hyperpolarized NMR.

# publications[].label
Remote detection of nuclear magnetic resonance with an anisotropic magnetoresistive sensor.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Quantitative rate determination by dynamic nuclear polarization enhanced NMR of a Diels-Alder reaction.

# publications[].label
Hyperpolarized binding pocket nuclear Overhauser effect for determination of competitive ligand binding.

# publications[].label
Intermolecular interactions determined by NOE build-up in macromolecules from hyperpolarized small molecules.

# publications[].label
Spontaneous emission of NMR signals in hyperpolarized proton spin systems.

# publications[].label
Implementation and characterization of flow injection in dissolution dynamic nuclear polarization NMR spectroscopy.

# publications[].label
Modeling of Polarization Transfer Kinetics in Protein Hydration Using Hyperpolarized Water.

# publications[].label
In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules.

# publications[].label
Applications of dissolution dynamic nuclear polarization in chemistry and biochemistry.

# publications[].label
Observation of Fast Two-Dimensional NMR Spectra during Protein Folding Using Polarization Transfer from Hyperpolarized Water.

# publications[].label
Application of blind source separation to real-time dissolution dynamic nuclear polarization.

# publications[].label
Rapid determination of biosynthetic pathways using fractional isotope enrichment and high-resolution dynamic nuclear polarization enhanced NMR.

# publications[].label
Characterization of Chemical Exchange Using Relaxation Dispersion of Hyperpolarized Nuclear Spins.

# publications[].label
NMR-derived dynamic aspects of N-type inactivation of a Kv channel suggest a transient interaction with the T1 domain.

# publications[].label
Controlling membrane permeability with bacterial porins: application to encapsulated enzymes.

# publications[].label
Quantifying the diffusion of a fluid through membranes by double phase encoded remote detection magnetic resonance imaging.

# publications[].label
Characterization of protein-ligand interactions by SABRE.

# publications[].label
Milli-tesla NMR and spectrophotometry of liquids hyperpolarized by dissolution dynamic nuclear polarization.

# publications[].label
Characterization of Membrane Protein-Lipid Interactions in Unfolded OmpX with Enhanced Time Resolution by Hyperpolarized NMR.

# publications[].label
Determination of protein-ligand binding modes using fast multi-dimensional NMR with hyperpolarization.

# publications[].label
Determination of Ligand Binding Epitope Structures Using Polarization Transfer from Hyperpolarized Ligands.

# researcherOn[].label
Development of Platform for Ligand Screening using Hyperpolarized NMR

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# researcherOn[].label
Hyperpolarization Assisted and Structure Based Screening of Protein-Ligand Interactions in Live Cells

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# researcherOn[].label
Characterization of Macromolecular Dynamics Using Para-hydrogen Induced Polarization of Nuclear Spins

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# streetAddress
Texas A&M University; Chemistry Department; 3255 TAMU

# etdChairOf[].label
Min, Hlaing (2014-08). Nuclear Magnetic Resonance based Characterization of the Protein Binding Pocket using Hyperpolarized Ligand. Master's Thesis.

# etdChairOf[].label
Chen, Hsueh-Ying (2015-05). Methods for Determination of Structure and Kinetics in the Study of Macromolecules Using Dissolution DNP NMR. Doctoral Dissertation.