# overview
Enzymes catalyze a remarkable variety of chemical reactions with extremely high rate enhancements and very selective substrate specificity. The research efforts in our laboratory are directed towards a more complete understanding of the fundamental principles involved in enzyme-catalyzed chemistry and the dependence on protein structure. The pursuit of this information will provide the framework for the rational and combinatorial redesign of these complex molecules in an effort to exploit and develop the properties of enzyme active sites for a variety of chemical, biological, and medicinal uses. The techniques that we are using to solve these problems include steady-state and stopped-flow kinetics, NMR and EPR spectroscopy, X-ray crystallography, and the synthesis of inhibitors and suicide substrates. We are also using recombinant DNA methods to construct new proteins with novel catalytic properties. These efforts are currently being directed to the reactions catalyzed by phosphotriesterase and enzymes involves in the degradation of lignin and the metabolism of novel carbohydrates from the human gut microbiome.

The phosphotriesterase enzyme catalyzes the hydrolysis of organophosphate insecticides and other toxic organophosphate nerve agents. We have discovered that the active site of this protein consists of a unique binuclear metal center for the activation of water. We are now investigating the structure and properties of this metal center as a model system for the evolution of enzyme structure and function. Toward this end we have mutated the active site of this enzyme in a research project to create novel enzymes with the ability to detect, destroy, and detoxify various chemical warfare agents such as sarin, soman, and VX. The Raushel laboratory is also engaged in a large scale research project that is focused on the development of novel strategies for the discovery of new enzymes.

# keywords[]
Carbon-nitrogen Ligases With Glutamine As Amide-n-donor

# keywords[]
Carbon-nitrogen Ligases With Glutamine As Amide-n-donor

# keywords[]
3102 Bioinformatics And Computational Biology

# keywords[]
1.1 Normal Biological Development And Functioning

# keywords[]
3404 Medicinal And Biomolecular Chemistry

# keywords[]
3214 Pharmacology And Pharmaceutical Sciences

# keywords[]
3407 Theoretical And Computational Chemistry

# keywords[]
3404 Medicinal And Biomolecular Chemistry

# keywords[]
3214 Pharmacology And Pharmaceutical Sciences

# keywords[]
3404 Medicinal And Biomolecular Chemistry

# keywords[]
3102 Bioinformatics And Computational Biology

# educationAndTraining[].organization.label
St. Thomas University - (Miami Gardens, Florida, United States)

# educationAndTraining[].organization.label
University of Wisconsin - Madison - (Madison, Wisconsin, United States)

# awardsAndHonors[].label
Gordon Hammes ACS Biochemistry Lectureship

# awardsAndHonors[].label
Repligen Award in Chemistry of Biological Processes

# publications[].label
Partial randomization of the four sequential amidation reactions catalyzed by cobyric acid synthetase with a single point mutation.

# publications[].label
Mechanism of cobyrinic acid a,c-diamide synthetase from Salmonella typhimurium LT2.

# publications[].label
Mechanism of the cytosine deaminase reacton

# publications[].label
High resolution X-ray structures of different metal-substituted forms of phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.

# publications[].label
Uronate isomerase: a nonhydrolytic member of the amidohydrolase superfamily with an ambivalent requirement for a divalent metal ion.

# publications[].label
Analysis of ping-pong reaction mechanisms by positional isotope exchange. Application to galactose-1-phosphate uridyltransferase.

# publications[].label
Pre-steady-state kinetics of carbamoyl phosphate synthetase of E-coli.

# publications[].label
Kinetic mechanism of kanamycin nucleotidyltransferase from Staphylococcus aureus

# publications[].label
Dissection of the functional domains of Escherichia coli carbamoyl phosphate synthetase by site-directed mutagenesis.

# publications[].label
A molecular wedge for triggering the amidotransferase activity of carbamoyl phosphate synthetase.

# publications[].label
Enzymatic deamination of the epigenetic base N-6-methyladenine.

# publications[].label
Folding pathways of circularly permuted ribonuclease T1.

# publications[].label
Regiochemical Analysis of the ProTide Activation Mechanism.

# publications[].label
Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis.

# publications[].label
Functional significance of Glu-77 and Tyr-137 within the active site of isoaspartyl dipeptidase.

# publications[].label
Carbamoyl phosphate synthetase: Three active sites connected by two molecular tunnels.

# publications[].label
Role of conserved residues within the carboxy phosphate domain of carbamoyl phosphate synthetase.

# publications[].label
Resolution of chiral phosphate, phosphonate, and phosphinate esters by an enantioselective enzyme library.

# publications[].label
Product Specificity of C4-Reductases in the Biosynthesis of GDP-6-Deoxy-Heptoses during Capsular Polysaccharide Formation in Campylobacter jejuni.

# publications[].label
Determination of the energetics of the UDP-glucose pyrophosphorylase reaction by positional isotope exchange inhibition.

# publications[].label
A positional isotope exchange study of the argininosuccinate lyase reaction.

# publications[].label
Characterization of the zinc binding site of bacterial phosphotriesterase.

# publications[].label
Functional identification of incorrectly annotated prolidases from the amidohydrolase superfamily of enzymes.

# publications[].label
At the periphery of the amidohydrolase superfamily: Bh0493 from Bacillus halodurans catalyzes the isomerization of D-galacturonate to D-tagaturonate.

# publications[].label
Crystallization and preliminary X-ray crystallographic analysis of carbamoyl phosphate synthetase from Escherichia coli.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Structures of the Carbon-Phosphorus Lyase Complex Reveal the Binding Mode of the NBD-Like PhnK

# publications[].label
Channeling of substrates and intermediates in enzyme-catalyzed reactions.

# publications[].label
Atropselective Hydrolysis of Chiral Binol-Phosphate Esters Catalyzed by the Phosphotriesterase from Sphingobium sp. TCM1.

# publications[].label
Enzyme-Catalyzed Kinetic Resolution of Chiral Precursors to Antiviral Prodrugs.

# publications[].label
Reaction mechanism of zinc-dependent cytosine deaminase from Escherichia coli: a quantum-chemical study.

# publications[].label
Differentiation of isotopically labeled nucleotides using fast atom bombardment tandem mass spectrometry.

# publications[].label
A versatile mechanism based reaction probe for the direct selection of biocatalysts

# publications[].label
TRANSPOSITION OF PROTEIN SEQUENCES - CIRCULAR PERMUTATION OF RIBONUCLEASE-T1

# publications[].label
The evolution of phosphotriesterase for decontamination and detoxification of organophosphorus chemical warfare agents.

# publications[].label
Kinetic evidence for the formation of D-alanyl phosphate in the mechanism of D-alanyl-D-alanine ligase.

# publications[].label
Identification of the histidine ligands to the binuclear metal center of phosphotriesterase by site-directed mutagenesis.

# publications[].label
Interrogation of the Substrate Profile and Catalytic Properties of the Phosphotriesterase from Sphingobium sp. Strain TCM1: An Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants and Plasticizers.

# publications[].label
RANDOM AND SITE DIRECTED MUTAGENESIS OF BACTERIAL LUCIFERASE

# publications[].label
Structure of a Novel Phosphotriesterase from Sphingobium sp. TCM1: A Familiar Binuclear Metal Center Embedded in a Seven-Bladed -Propeller Protein Fold.

# publications[].label
Tight binding inhibitors of N-acyl amino sugar and N-acyl amino acid deacetylases.

# publications[].label
Carbamoyl-phosphate synthetase. Creation of an escape route for ammonia.

# publications[].label
Self-assembly of the binuclear metal center of phosphotriesterase.

# publications[].label
Interaction of bacterial luciferase with 8-substituted flavin mononucleotide derivatives (vol 271, pg 104, 1996)

# publications[].label
The ferrous-dioxy complex of neuronal nitric-oxide synthase: Divergent effects of L-arginine and tetrahydrobiopterin on its stability

# publications[].label
Biosynthesis of 3,6-Dideoxy-heptoses for the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Catalytic properties of the PepQ prolidase from Escherichia coli.

# publications[].label
METHYL CHYMOTRYPSIN CATALYZED HYDROLYSES OF SPECIFIC SUBSTRATE ESTERS INDICATE MULTIPLE PROTON CATALYSIS IS POSSIBLE WITH A MODIFIED CHARGE RELAY TRIAD

# publications[].label
Stopped-flow kinetic analysis of the bacterial luciferase reaction.

# publications[].label
Intramolecular epistasis correlates with divergence of specificity in promiscuous and bifunctional NSAR/OSBS enzymes.

# publications[].label
Annotating enzymes of uncertain function: the deacylation of D-amino acids by members of the amidohydrolase superfamily.

# publications[].label
Stereochemical specificity of organophosphorus acid anhydrolase toward p-nitrophenyl analogs of soman and sarin.

# publications[].label
A novel multistep mechanism for oxygen binding to ferrous hemoproteins: rapid kinetic analysis of ferrous-dioxy myeloperoxidase (compound III) formation.

# publications[].label
THE EFFECTS OF MUTATIONS AT TRP131, LYS169, AND ASP253 ON PHOSPHOTRIESTERASE ACTIVITY

# publications[].label
Altering the substrate specificity of enzymes in the amidohydrolase superfamily

# publications[].label
Discovery of a Previously Unrecognized Ribonuclease from Escherichia coli That Hydrolyzes 5'-Phosphorylated Fragments of RNA.

# publications[].label
Second-Shell Amino Acid R266 Helps Determine N-Succinylamino Acid Racemase Reaction Specificity in Promiscuous N-Succinylamino Acid Racemase/o-Succinylbenzoate Synthase Enzymes.

# publications[].label
The amidotransferase family of enzymes: molecular machines for the production and delivery of ammonia.

# publications[].label
A multinuclear nuclear magnetic resonance study of the monovalent-divalent cation sites of pyruvate kinase.

# publications[].label
Carbamoyl phosphate synthetase from Escherichia coli does not catalyze the dehydration of bicarbonate to carbon dioxide

# publications[].label
Chemical and kinetic evidence for an essential histidine in the phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Functional annotation of two new carboxypeptidases from the amidohydrolase superfamily of enzymes.

# publications[].label
Annotating enzymes of unknown function: N-formimino-L-glutamate deiminase is a member of the amidohydrolase superfamily.

# publications[].label
Evolution of function in (beta/alpha)8-barrel enzymes.

# publications[].label
MECHANISM OF THE ARGININOSUCCINATE SYNTHETASE REACTION

# publications[].label
The binding of substrate analogs to phosphotriesterase.

# publications[].label
Inhibitors directed towards the binuclear metal center of phosphotriesterase (vol 12, pg 191, 1997)

# publications[].label
Function discovery and structural characterization of a methylphosphonate esterase.

# publications[].label
Isolation, purification, and characterization of a putative amidohydrolase superfamily enzyme from Escherichia coli

# publications[].label
The three dimensional structure of carbamoyl phosphate synthetase

# publications[].label
The mechanism of glycogen synthetase as determined by deuterium isotope effects and positional isotope exchange experiments.

# publications[].label
Acid-base catalysis in the argininosuccinate lyase reaction.

# publications[].label
An OPAA enzyme mutant with increased catalytic efficiency on the nerve agents sarin, soman, and GP.

# publications[].label
Identification of a phosphorylated enzyme intermediate in the catalytic mechanism for selenophosphate synthetase

# publications[].label
Mechanism of the dihydroorotase reaction.

# publications[].label
Intrinsic GTPase Activity of K-RAS Monitored by Native Mass Spectrometry.

# publications[].label
Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12.

# publications[].label
Biosynthesis of Nucleoside Diphosphoramidates in Campylobacter jejuni.

# publications[].label
Differential roles for three conserved histidine residues within the large subunit of carbamoyl phosphate synthetase.

# publications[].label
Long-range allosteric transitions in carbamoyl phosphate synthetase.

# publications[].label
Stereoselective detoxification of chiral sarin and soman analogues by phosphotriesterase.

# publications[].label
Stereochemical preferences for chiral substrates by the bacterial phosphotriesterase.

# publications[].label
Substrate Distortion and the Catalytic Reaction Mechanism of 5-Carboxyvanillate Decarboxylase.

# publications[].label
INVESTIGATION OF RIBONUCLEASE-T1 FOLDING INTERMEDIATES BY HYDROGEN-DEUTERIUM AMIDE EXCHANGE - 2D-NMR SPECTROSCOPY

# publications[].label
MECHANISM OF THE ARGININOSUCCINATE SYNTHETASE REACTION

# publications[].label
l-Galactose metabolism in Bacteroides vulgatus from the human gut microbiota.

# publications[].label
Biophysical applications of NMR to phosphoryl transfer enzymes and metal nuclei of metalloproteins.

# publications[].label
Structural Analysis of Cj1427, an Essential NAD-Dependent Dehydrogenase for the Biosynthesis of the Heptose Residues in the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Structures of the Carbon-Phosphorus Lyase Complex Reveal the Binding Mode of the NBD-like PhnK.

# publications[].label
Expression of Pseudomonas phosphotriesterase activity in the fall armyworm confers resistance to insecticides.

# publications[].label
Phosphotriesterase--a promising candidate for use in detoxification of organophosphates.

# publications[].label
Evolution of enzymatic activities in the enolase superfamily: N-succinylamino acid racemase and a new pathway for the irreversible conversion of D- to L-amino acids.

# publications[].label
Substrate and stereochemical specificity of the organophosphorus acid anhydrolase from Alteromonas sp. JD6.5 toward p-nitrophenyl phosphotriesters.

# publications[].label
Roles of three conserved residues in the small subunit of carbamoyl phosphate synthetase

# publications[].label
Structural defects within the carbamate tunnel of carbamoyl phosphate synthetase.

# publications[].label
Investigation of phosphotriesterase binuclear metal site by electron paramagnetic resonance.

# publications[].label
Bifunctional Epimerase/Reductase Enzymes Facilitate the Modulation of 6-Deoxy-Heptoses Found in the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Textile-based wearable solid-contact flexible fluoride sensor: Toward biodetection of G-type nerve agents.

# publications[].label
Identification and Functional Characterization of the Polymerizing Glycosyltransferase Required for the Transfer of d-Ribose to the d-GalfNAc Moiety of the Capsular Polysaccharide of Campylobacter jejuni.

# publications[].label
Substrate deconstruction and the nonadditivity of enzyme recognition.

# publications[].label
Variants of Phosphotriesterase for the Enhanced Detoxification of the Chemical Warfare Agent VR.

# publications[].label
Discovery of a cAMP deaminase that quenches cyclic AMP-dependent regulation.

# publications[].label
Circular permutations of ribonuclease T1

# publications[].label
Variants of phosphotriesterase for the hydrolysis and detoxification of nerve agents

# publications[].label
Nitro analogs of substrates for argininosuccinate synthetase and argininosuccinate lyase.

# publications[].label
Pre-steady-state kinetics reveal a slow isomerization of the enzyme-NAD complex in the NAD-malic enzyme reaction.

# publications[].label
Success of pyridostigmine, physostigmine, eptastigmine and phosphotriesterase treatments in acute sarin intoxication.

# publications[].label
Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site.

# publications[].label
Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center.

# publications[].label
Structural and Functional Characterization of YdjI, an Aldolase of Unknown Specificity in Escherichia coli K12.

# publications[].label
Detoxification of organophosphate pesticides using an immobilized phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Substrate synergism and the kinetic mechanism of yeast hexokinase.

# publications[].label
Variants of phosphotriesterase for the hydrolysis and detoxification of nerve agents

# publications[].label
THE BINUCLEAR METAL CENTER AT THE ACTIVE-SITE OF THE BACTERIAL PHOSPHOTRIESTERASE

# publications[].label
Enzymes for the homeland defense: optimizing phosphotriesterase for the hydrolysis of organophosphate nerve agents.

# publications[].label
Stereochemical constraints on the catalytic hydrolysis of organophosphate nerve agents by phosphotriesterase

# publications[].label
Deuterium kinetic isotope effects and the mechanism of the bacterial luciferase reaction.

# publications[].label
D-Ala-D-X ligases: evaluation of D-alanyl phosphate intermediate by MIX, PIX and rapid quench studies.

# publications[].label
High-resolution X-ray structure of isoaspartyl dipeptidase from Escherichia coli.

# publications[].label
KINETIC MECHANISM OF BOVINE LIVER FRUCTOKINASE

# publications[].label
Quantifying the allosteric properties of Escherichia coli carbamyl phosphate synthetase: Determination of thermodynamic linked-function parameters in an ordered kinetic mechanism

# publications[].label
The substrate and anomeric specificity of fructokinase.

# publications[].label
Determination of the mechanism of the argininosuccinate synthetase reaction by static and dynamic quench experiments.

# publications[].label
Biosynthesis of d-glycero-l-gluco-Heptose in the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Deamination of 6-aminodeoxyfutalosine in menaquinone biosynthesis by distantly related enzymes.

# publications[].label
PARTITIONING OF INTERMEDIATES IN THE ARGININOSUCCINATE SYNTHETASE REACTION

# publications[].label
Mechanism and stereochemical course at phosphorus of the reaction catalyzed by a bacterial phosphotriesterase.

# publications[].label
Phosphotriesterase, pralidoxime-2-chloride (2-PAM) and eptastigmine treatments and their combinations in DFP intoxication.

# publications[].label
Hydrolysis of phosphotriesters: determination of transition states in parallel reactions by heavy-atom isotope effects.

# publications[].label
The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway.

# publications[].label
Structural and catalytic diversity within the amidohydrolase superfamily.

# publications[].label
Functional Characterization of YdjH, a Sugar Kinase of Unknown Specificity in Escherichia coli K12.

# publications[].label
Enzymatic Synthesis of an Undecorated Capsular Polysaccharide from Campylobacter jejuni.

# publications[].label
Phosphorus-31 nuclear magnetic resonance application to positional isotope exchange reactions catalyzed by Escherichia coli carbamoyl-phosphate synthetase: analysis of forward and reverse enzymatic reactions.

# publications[].label
Metabolic Pathways for the Biosynthesis of Heptoses Used in the Construction of Capsular Polysaccharides in the Human Pathogen Campylobacter jejuni.

# publications[].label
INVESTIGATION OF THE ACTIVE-SITE AND METAL CENTERS OF PHOSPHOTRIESTERASE

# publications[].label
On the catalytic mechanism of human ATP citrate lyase.

# publications[].label
Enantioselectivity of phosphotriesterase and G60A mutant analogue for chiral organophosphates.

# publications[].label
Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu-58----Ala.

# publications[].label
Identification and Characterization of the Two Glycosyltransferases Required for the Polymerization of the HS:1 Serotype Capsular Polysaccharide of Campylobacter jejuni G1.

# publications[].label
Hydrolysis of phosphodiesters through transformation of the bacterial phosphotriesterase.

# publications[].label
The catalytic mechanism of galactose mutarotase.

# publications[].label
Role of the hinge loop linking the N- and C-terminal domains of the amidotransferase subunit of carbamoyl phosphate synthetase.

# publications[].label
Structural and functional analyses of phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Synthesis and kinetic analysis of inhibitors and inactivators of phosphotriesterase

# publications[].label
Virtual screening against metalloenzymes for inhibitors and substrates.

# publications[].label
A DIRECT NMR METHOD FOR THE DETERMINATION OF CORRELATION TIMES IN ENZYME COMPLEXES INVOLVING MONO-VALENT CATIONS AND PARAMAGNETIC CENTERS

# publications[].label
Measurement of positional isotope exchange rates in enzyme-catalyzed reactions by fast atom bombardment mass spectrometry: application to argininosuccinate synthetase.

# publications[].label
Mechanism-based inactivation of rabbit muscle phosphoglucomutase by nojirimycin 6-phosphate.

# publications[].label
Utilization of copper as a paramagnetic probe for the binuclear metal center of phosphotriesterase.

# publications[].label
Deciphering the Aldolase Function of STM3780 from a Bovine Enteric Infection-Related Gene Cluster in Salmonella enterica Serotype Typhimurium.

# publications[].label
Functional Annotation of LigU as a 1,3-Allylic Isomerase during the Degradation of Lignin in the Protocatechuate 4,5-Cleavage Pathway from the Soil Bacterium Sphingobium sp. SYK-6.

# publications[].label
Structure-guided discovery of new deaminase enzymes.

# publications[].label
Determining reactivity and stereospecificity of phosphotriesterase from Pseudonomas diminuta by site-specific mutagenesis

# publications[].label
Presteady-state kinetics of carbamoyl phosphate synthetase of E. coli.

# publications[].label
Structure of carbamoyl phosphate synthetase: a journey of 96 A from substrate to product.

# publications[].label
The binding of inosine monophosphate to Escherichia coli carbamoyl phosphate synthetase.

# publications[].label
Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases from the Amidohydrolase Superfamily.

# publications[].label
Allosteric control of the oligomerization of carbamoyl phosphate synthetase from Escherichia coli.

# publications[].label
Discovery of an L-fucono-1,5-lactonase from cog3618 of the amidohydrolase superfamily.

# publications[].label
CO2 IS REQUIRED FOR THE ASSEMBLY OF THE BINUCLEAR METAL CENTER OF PHOSPHOTRIESTERASE

# publications[].label
The monovalent cation site of pyruvate kinase and other enzymes: NMR investigations.

# publications[].label
Experimental verification of a predicted, previously unseen separation selectivity pattern in the capillary electrophoretic separation of noncharged enantiomers by octakis(2,3-diacetyl-6-sulfato)-gamma-cyclodextrin.

# publications[].label
Carbamoyl phosphate synthetase: a crooked path from substrates to products.

# publications[].label
NMR-STUDIES OF THE MONO-VALENT CATION SITE OF PYRUVATE-KINASE

# publications[].label
Evidence for the utilization of formate as a substrate by carbamoyl phosphate synthetase

# publications[].label
Stereoselective hydrolysis of organophosphate nerve agents by the bacterial phosphotriesterase.

# publications[].label
MECHANISM OF THE BACTERIAL LUCIFERASE REACTION

# publications[].label
An empirical analysis of enzyme function reporting for experimental reproducibility: Missing/incomplete information in published papers.

# publications[].label
Positional isotope exchange analysis of the pantothenate synthetase reaction.

# publications[].label
Structure and Chemical Reaction Mechanism of LigU, an Enzyme That Catalyzes an Allylic Isomerization in the Bacterial Degradation of Lignin.

# publications[].label
Discovery of a cyclic phosphodiesterase that catalyzes the sequential hydrolysis of both ester bonds to phosphorus.

# publications[].label
Carbamyl phosphate synthetase of Escherichia coli uses the same diastereomer of adenosine-5'-[2-thiotriphosphate] at both ATP sites.

# publications[].label
The determination of enzyme-substrate dissociation rates by dynamic isotope exchange enhancement experiments.

# publications[].label
Perturbations to the active site of phosphotriesterase.

# publications[].label
Theoretical investigation of the reaction mechanism of the dinuclear zinc enzyme dihydroorotase.

# publications[].label
Deconstruction of the catalytic array within the amidotransferase subunit of carbamoyl phosphate synthetase.

# publications[].label
Dissection of the conduit for allosteric control of carbamoyl phosphate synthetase by ornithine.

# publications[].label
Distances between structural metal ion, substrates, and allosteric modifier of fructose bisphosphatase.

# publications[].label
A combined theoretical and experimental study of the ammonia tunnel in carbamoyl phosphate synthetase.

# publications[].label
Functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily.

# publications[].label
Differentiation of chiral phosphorus enantiomers by P and H NMR spectroscopy using amino acid derivatives as chemical solvating agents.

# publications[].label
MUTATIONAL ANALYSIS OF 2 PUTATIVE DOMAINS WITHIN THE LARGE SUBUNIT OF CARBAMOYL PHOSPHATE SYNTHETASE FROM ESCHERICHIA-COLI

# publications[].label
Cytidine Diphosphoramidate Kinase: An Enzyme Required for the Biosynthesis of the O-Methyl Phosphoramidate Modification in the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
KINETIC MECHANISM OF BACTERIAL LUCIFERASE

# publications[].label
A stringent test for the nucleotide switch mechanism of carbamoyl phosphate synthetase.

# publications[].label
Interaction of bacterial luciferase with 8-substituted flavin mononucleotide derivatives.

# publications[].label
MODULATION OF THE ALLOSTERIC CONTROL DOMAIN OF CARBAMOYL-PHOSPHATE SYNTHETASE

# publications[].label
RATE LIMITING STEPS OF CARBAMYL-PHOSPHATE SYNTHETASE OF ESCHERICHIA-COLI

# publications[].label
Roles of the second-shell amino acid R266 in other members of the MLE subgroup of the enolase superfamily

# publications[].label
The interaction of monofluorofumarate with adenylosuccinate lyase.

# publications[].label
Enzymes within the amidohydrolase superfamily from Mycobacterium tuberculosis

# publications[].label
Restricted passage of reaction intermediates through the ammonia tunnel of carbamoyl phosphate synthetase.

# publications[].label
Substrate Profile of the Phosphotriesterase Homology Protein from Escherichia coli.

# publications[].label
Determination of the rate-limiting steps and chemical mechanism of fructokinase by isotope exchange, isotope partitioning, and pH studies.

# publications[].label
Potent inhibition of the C-P lyase nucleosidase PhnI by Immucillin-A triphosphate.

# publications[].label
Rescue of the orphan enzyme isoguanine deaminase.

# publications[].label
Characterization of a phosphodiesterase capable of hydrolyzing EA 2192, the most toxic degradation product of the nerve agent VX.

# publications[].label
Stereochemical probes of the argininosuccinate synthetase reaction.

# publications[].label
Analysis of the galactosyltransferase reaction by positional isotope exchange and secondary deuterium isotope effects.

# publications[].label
Conformational stability of ribonuclease T1 determined by hydrogen-deuterium exchange.

# publications[].label
SYNTHESIS AND ENZYMATIC-HYDROLYSIS OF A LIGHT-EMITTING SUBSTRATE FOR PHOSPHOTRIESTERASE

# publications[].label
Mechanism-based inactivation of phosphotriesterase by reaction of a critical histidine with a ketene intermediate.

# publications[].label
Catalytic mechanism and three-dimensional structure of adenine deaminase.

# publications[].label
Manganese-Induced Substrate Promiscuity in the Reaction Catalyzed by Phosphoglutamine Cytidylyltransferase from Campylobacter jejuni.

# publications[].label
Conformational stability of ribonuclease T1 determined by hydrogen-deuterium exchange.

# publications[].label
Inactivation of the amidotransferase activity of carbamoyl phosphate synthetase by the antibiotic acivicin.

# publications[].label
An engineered blockage within the ammonia tunnel of carbamoyl phosphate synthetase prevents the use of glutamine as a substrate but not ammonia.

# publications[].label
Transition State Analysis of the Reaction Catalyzed by the Phosphotriesterase from Sphingobium sp. TCM1.

# publications[].label
Subunit Interactions within the Carbon-Phosphorus Lyase Complex from Escherichia coli.

# publications[].label
Deciphering substrate specificity for enzymes of unknown function

# publications[].label
Structural and mechanistic characterization of L-histidinol phosphate phosphatase from the polymerase and histidinol phosphatase family of proteins.

# publications[].label
Circular permutation of RNase T1 through PCR based site-directed mutagenesis

# publications[].label
Inhibitor binding to the Phe53Trp mutant of HIV-1 protease promotes conformational changes detectable by spectrofluorometry.

# publications[].label
Are turns required for the folding of ribonuclease T1?

# publications[].label
Sensitivity and specificity improvement of an ion sensitive field effect transistors-based biosensor for potato glycoalkaloids detection.

# publications[].label
Structural determinants of the substrate and stereochemical specificity of phosphotriesterase.

# publications[].label
Carbamoyl phosphate synthetase: a tunnel runs through it.

# publications[].label
Phosphorus-31 NMR relaxation studies of diethyl p-methoxyphenyl phosphate bound to phosphotriesterase

# publications[].label
Crystal structure of cytosine deaminase complexed with a mimic of the tetrahedral intermediate

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Three-dimensional structure and catalytic mechanism of cytosine deaminase.

# publications[].label
Biochemistry. Not an oxidase, but a peroxidase.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Determination of rate-limiting steps of Escherichia coli carbamoyl-phosphate synthase. Rapid quench and isotope partitioning experiments.

# publications[].label
S-adenosylmethionine: studies on chemical and enzymatic synthesis.

# publications[].label
Mechanism of cobyrinic acid a,c-diamide synthetase from Salmonella typhimurium

# publications[].label
Three-dimensional structure of phosphotriesterase: an enzyme capable of detoxifying organophosphate nerve agents.

# publications[].label
The catalase activity of diiron adenine deaminase.

# publications[].label
Functional Characterization of Cj1427, a Unique Ping-Pong Dehydrogenase Responsible for the Oxidation of GDP-d-glycero--d-manno-heptose in Campylobacter jejuni.

# publications[].label
A Combined Experimental-Theoretical Study of the LigW-Catalyzed Decarboxylation of 5-Carboxyvanillate in the Metabolic Pathway for Lignin Degradation

# publications[].label
Hydrolysis of phosphodiesters by the bacterial phosphotriesterase

# publications[].label
The ferrous-dioxy complex of neuronal nitric oxide synthase. Divergent effects of L-arginine and tetrahydrobiopterin on its stability.

# publications[].label
The differentially conserved residues of carbamoyl-phosphate synthetase.

# publications[].label
Functional Characterization of the ycjQRS Gene Cluster from Escherichia coli: A Novel Pathway for the Transformation of d-Gulosides to d-Glucosides.

# publications[].label
MECHANISM-BASED INACTIVATION OF A BACTERIAL PHOSPHOTRIESTERASE BY AN ALKYNYL PHOSPHATE ESTER

# publications[].label
Enzymatic synthesis of chiral organophosphothioates from prochiral precursors.

# publications[].label
Structure, mechanism, and substrate profile for Sco3058: the closest bacterial homologue to human renal dipeptidase .

# publications[].label
Assignment of pterin deaminase activity to an enzyme of unknown function guided by homology modeling and docking.

# publications[].label
Transmutation of the amidohydrolase superfamily

# publications[].label
Multiple Reaction Products from the Hydrolysis of Chiral and Prochiral Organophosphate Substrates by the Phosphotriesterase from Sphingobium sp. TCM1.

# publications[].label
Kinetic mechanism of bovine liver argininosuccinate lyase.

# publications[].label
STRENDA DB: enabling the validation and sharing of enzyme kinetics data.

# publications[].label
Channeling of ammonia through the intermolecular tunnel contained within carbamoyl phosphate synthetase

# publications[].label
Kinetic evidence supports the existence of two halide binding sites that have a distinct impact on the heme iron microenvironment in myeloperoxidase.

# publications[].label
Mechanism and Structure of -Resorcylate Decarboxylase.

# publications[].label
Three-dimensional structure of the zinc-containing phosphotriesterase with the bound substrate analog diethyl 4-methylbenzylphosphonate.

# publications[].label
Regulatory control of the amidotransferase domain of carbamoyl phosphate synthetase.

# publications[].label
Capillary electrophoretic separation of the enantiomers of organophosphates with a phosphorus stereogenic center using the sodium salt of octakis(2,3-diacetyl-6-sulfo)-gamma-cyclodextrin as resolving agent.

# publications[].label
Mechanism for the transport of ammonia within carbamoyl phosphate synthetase determined by molecular dynamics simulations.

# publications[].label
Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine.

# publications[].label
Prospecting for unannotated enzymes: discovery of a 3',5'-nucleotide bisphosphate phosphatase within the amidohydrolase superfamily.

# publications[].label
Isotopic probes of the argininosuccinate lyase reaction.

# publications[].label
Bovine liver fructokinase: purification and kinetic properties.

# publications[].label
Harnessing the catalytic potential of the binuclear metal center from phosphotriesterase

# publications[].label
Histidine-254 is essential for the inactivation of phosphotriesterase with the alkynyl phosphate esters and diethyl pyrocarbonate.

# publications[].label
Alterations in the energetics of the carbamoyl phosphate synthetase reaction by site-directed modification of the essential sulfhydryl group.

# publications[].label
Stereoselective Formation of Multiple Reaction Products by the Phosphotriesterase from Sphingobium sp. TCM1.

# publications[].label
Discovery of a bacterial 5-methylcytosine deaminase.

# publications[].label
Synchronization of the three reaction centers within carbamoyl phosphate synthetase.

# publications[].label
Inhibitors directed towards the binuclear metal center of phosphotriesterase.

# publications[].label
Enzymatic resolution of chiral phosphinate esters.

# publications[].label
Correction to Structure of a Novel Phosphotriesterase from Sphingobium sp. TCM1: A Familiar Binuclear Metal Center Embedded in a Seven-Bladed -Propeller Protein Fold.

# publications[].label
Biosynthesis of CDP--d-fucofuranose and CDP--l-6-deoxy-altrofuranose for the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Discovery of a Glutamine Kinase Required for the Biosynthesis of the O-Methyl Phosphoramidate Modifications Found in the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Discovery and Functional Characterization of a Clandestine ATP-Dependent Amidoligase in the Biosynthesis of the Capsular Polysaccharide from Campylobacter jejuni.

# publications[].label
Structure and Reaction Mechanism of YcjR, an Epimerase That Facilitates the Interconversion of d-Gulosides to d-Glucosides in Escherichia coli.

# publications[].label
The catalytic mechanism for aerobic formation of methane by bacteria.

# publications[].label
Discovery and structure determination of the orphan enzyme isoxanthopterin deaminase .

# publications[].label
Detection of intermediates in the carbamoyl phosphate synthetase reaction

# publications[].label
Investigation of ribonuclease T1 folding intermediates by hydrogen-deuterium amide exchange-two-dimensional NMR spectroscopy.

# publications[].label
Erratum: Interaction of bacterial luciferase with 8-substituted flavin mononucleotide derivatives (Journal of Biological Chemistry (1996) 271 (104- 110))

# publications[].label
The mechanism of the reaction catalyzed by uronate isomerase illustrates how an isomerase may have evolved from a hydrolase within the amidohydrolase superfamily.

# publications[].label
Allosteric dominance in carbamoyl phosphate synthetase.

# publications[].label
Carbamoyl phosphate synthetase: an amazing biochemical odyssey from substrate to product.

# publications[].label
Positional isotope exchange analysis of the Mycobacterium smegmatis cysteine ligase (MshC).

# publications[].label
Mapping the structural domains of E. coli carbamoyl phosphate synthetase using limited proteolysis.

# publications[].label
Stereochemistry of binding of thiophosphate analogs of ATP and ADP to carbamate kinase, glutamine synthetase, and carbamoyl-phosphate synthetase.

# publications[].label
Tunneling of intermediates in enzyme-catalyzed reactions.

# publications[].label
INACTIVATION OF BOVINE LIVER ARGININOSUCCINATE LYASE BY MONOFLUOROFUMARATE AND DIFLUOROFUMARATE

# publications[].label
CARBAMOYL PHOSPHATE SYNTHETASE: A LONG JOURNEY HOME

# publications[].label
Positional isotope exchange as probe of enzyme action.

# publications[].label
Overcoming the Challenges of Enzyme Evolution To Adapt Phosphotriesterase for V-Agent Decontamination.

# publications[].label
Phosphotriesterase: an enzyme in search of its natural substrate.

# publications[].label
Deciphering the Enzymatic Function of the Bovine Enteric Infection-Related Protein YfeJ from Salmonella enterica Serotype Typhimurium.

# publications[].label
Mechanism-based inhibitors for the inactivation of the bacterial phosphotriesterase.

# publications[].label
Stereospecificity in the enzymatic hydrolysis of cyclosarin (GF)

# publications[].label
Correction to "Functional Characterization of Two PLP-Dependent Enzymes Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni".

# publications[].label
Standards for Reporting Enzyme Data: The STRENDA Consortium: What it aims to do and why it should be helpful

# publications[].label
Rationally engineered mutants of phosphotriesterase for preparative scale isolation of chiral organophosphates

# publications[].label
Enzymatic neutralization of the chemical warfare agent VX: evolution of phosphotriesterase for phosphorothiolate hydrolysis.

# publications[].label
Carbamate transport in carbamoyl phosphate synthetase: a theoretical and experimental investigation.

# publications[].label
Mechanism of the reaction catalyzed by isoaspartyl dipeptidase from Escherichia coli.

# publications[].label
A nuclear magnetic resonance study of the topography of binding sites of Escherichia coli carbamoyl-phosphate synthetase.

# publications[].label
Structure and Reaction Mechanism of the LigJ Hydratase: An Enzyme Critical for the Bacterial Degradation of Lignin in the Protocatechuate 4,5-Cleavage Pathway.

# publications[].label
Kinetic mechanism of the bacterial luciferase reaction

# publications[].label
Substrate Specificity and Chemical Mechanism for the Reaction Catalyzed by Glutamine Kinase.

# publications[].label
Kinetic destabilization of the hydroperoxy flavin intermediate by site-directed modification of the reactive thiol in bacterial luciferase.

# publications[].label
Influence of primary sequence transpositions on the folding pathways of ribonuclease T1.

# publications[].label
Erratum: Phosphorus-31 nuclear magnetic resonance application to positional isotope exchange reactions catalyzed by Escherichia coli carbamoyl-phosphate synthetase: Analysis of forward and reverse enzymatic reactions (Biochemistry (1980) 19, 14, (3170-3174))

# publications[].label
Stereochemical constraints on the substrate specificity of phosphotriesterase.

# publications[].label
Biosynthesis of Cytidine Diphosphate-6-d-Glucitol for the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
A common catalytic mechanism for proteins of the HutI family.

# publications[].label
Intermediates in the transformation of phosphonates to phosphate by bacteria.

# publications[].label
The multiple amidation reactions catalyzed by Cobyric acid synthetase from Salmonella typhimurium are sequential and dissociative.

# publications[].label
STRUCTURAL STUDIES ON CARBAMOYL PHOSPHATE SYNTHETASE.

# publications[].label
ATPASE REACTION ENERGETICS OF ESCHERICHIA-COLI CPS MUTANT AND WILD-TYPE ENZYMES

# publications[].label
Kinetic mechanism of Escherichia coli carbamoyl-phosphate synthetase.

# publications[].label
Identification and structure determination of novel substituted purine deaminase enzymes

# publications[].label
FORMATION OF SECONDARY STRUCTURE OF RIBONUCLEASE-T1

# publications[].label
ON THE MECHANISM OF THE PHOSPHOTRIESTERASE REACTION

# publications[].label
Activation of the binuclear metal center through formation of phosphotriesterase-inhibitor complexes.

# publications[].label
A combinatorial library for the binuclear metal center of bacterial phosphotriesterase.

# publications[].label
Functional Characterization of Two PLP-Dependent Enzymes Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.

# publications[].label
Inhibitors designed for the active site of dihydroorotase.

# publications[].label
Protonation of the binuclear metal center within the active site of phosphotriesterase.

# publications[].label
Structural characterization of the divalent cation sites of bacterial phosphotriesterase by 113Cd NMR spectroscopy.

# publications[].label
Structure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily .

# publications[].label
P-7 THE FERROUS-DIOXY COMPLEX OF NEURONAL NITRIC-OXIDE SYNTHASE: DIVERGENT EFFECTS OF L-ARGININE AND TETRAHYDROBIOPTERIN ON ITS STABILITY

# publications[].label
The enzymatic conversion of phosphonates to phosphate by bacteria.

# publications[].label
Engineered variants of phosphotriesterase for enhanced detoxification of organophosphate nerve agents

# publications[].label
Predicting substrates by docking high-energy intermediates to enzyme structures.

# publications[].label
ATPASE REACTION ENERGETICS OF ESCHERICHIA-COLI CPS MUTANT AND WILD-TYPE ENZYMES

# publications[].label
Metal-substrate interactions facilitate the catalytic activity of the bacterial phosphotriesterase.

# publications[].label
The structure of carbamoyl phosphate synthetase determined to 2.1 A resolution.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Biosynthesis of UDP--N-Acetyl-d-mannosaminuronic Acid and CMP--N-Acetyl-d-neuraminic Acid for the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
Organophosphate nerve agent toxicity in Hydra attenuata.

# publications[].label
STRUCTURAL AND FUNCTIONAL ANALYSES OF DIHYDROOROTASE FROM ESCHERICHIA COLI

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Role of the four conserved histidine residues in the amidotransferase domain of carbamoyl phosphate synthetase.

# publications[].label
Structure of diethyl phosphate bound to the binuclear metal center of phosphotriesterase.

# publications[].label
Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution.

# publications[].label
Kinetics of the -glutamyl thioester intermediate in the glutamine amidotransferase activity of carbamoyl phosphate synthetase of E-coli

# publications[].label
Structural characterization and function determination of a nonspecific carboxylate esterase from the amidohydrolase superfamily with a promiscuous ability to hydrolyze methylphosphonate esters.

# publications[].label
COMP 371-Predicting enzyme function by docking high-energy intermediates of potential substrates

# publications[].label
Extreme promiscuity: Adenine deaminase from Escherichia coli catalyzes a hydrolytic and a redox reaction.

# publications[].label
ANTIFERROMAGNETIC COUPLING IN THE BINUCLEAR METAL CLUSTER OF MANGANESE-SUBSTITUTED PHOSPHOTRIESTERASE

# publications[].label
Access to the carbamate tunnel of carbamoyl phosphate synthetase.

# publications[].label
Structural diversity within the mononuclear and binuclear active sites of N-acetyl-D-glucosamine-6-phosphate deacetylase.

# publications[].label
CALCULATION OF RETENTION INDEXES FOR BENZENE AND BENZENE DERIVATIVES ON BASIS OF MOLECULAR STRUCTURE

# publications[].label
Transition-state structures for enzymatic and alkaline phosphotriester hydrolysis.

# publications[].label
A POSITIONAL ISOTOPE EXCHANGE ANALYSIS OF THE URIDINE-DIPHOSPHOGLUCOSE PYROPHOSPHORYLASE REACTION

# publications[].label
Augmented hydrolysis of diisopropyl fluorophosphate in engineered mutants of phosphotriesterase.

# publications[].label
Biosynthesis of UDP--l-Arabinofuranoside for the Capsular Polysaccharides of Campylobacter jejuni.

# publications[].label
C3- and C3/C5-Epimerases Required for the Biosynthesis of the Capsular Polysaccharides from Campylobacter jejuni.

# publications[].label
Phosphotriesterase decreases paraoxon toxicity in mice.

# publications[].label
Chemical Mechanism of the Phosphotriesterase from Sphingobium sp. Strain TCM1, an Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants.

# publications[].label
Molecular engineering of organophosphate hydrolysis activity from a weak promiscuous lactonase template.

# publications[].label
Structural determinants for the stereoselective hydrolysis of chiral substrates by phosphotriesterase.

# publications[].label
Roles of hinge loop linking N- and C-terminal domains of small subunit of carbamoyl phosphate synthetase from Escherichia coli.

# publications[].label
KINETIC-ANALYSIS OF CARBAMOYL PHOSPHATE SYNTHETASE MUTANTS

# publications[].label
Proposed mechanism for the bacterial bioluminescence reaction involving a dioxirane intermediate.

# publications[].label
Carbamoyl phosphate synthetase: closure of the B-domain as a result of nucleotide binding.

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Biosynthesis of GDP-d-glycero--d-manno-heptose for the Capsular Polysaccharide of Campylobacter jejuni.

# publications[].label
Functional Characterization of a HAD Phosphatase Involved in Capsular Polysaccharide Biosynthesis in Campylobacter jejuni.

# publications[].label
Pre-Steady-state kinetics reveal a slow isomerization of the enzyme-NAD complex in the NAD-malic enzyme reaction

# publications[].label
Three-dimensional structure of the binuclear metal center of phosphotriesterase.

# publications[].label
Limits of diffusion in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Effect of linker sequence on the stability of circularly permuted variants of ribonuclease T1.

# publications[].label
STRUCTURE-REACTIVITY RELATIONSHIPS FOR THE HYDROLYSIS OF SUBSTRATES BY THE BACTERIAL PHOSPHOTRIESTERASE

# publications[].label
Paramagnetic probes for carbamoyl-phosphate synthetase: metal ion binding studies and preparation of nitroxide spin-labeled derivatives.

# publications[].label
The use of phosphotriesterase in the synthesis of enantiomerically pure ProTide prodrugs.

# publications[].label
Structure-based function discovery of an enzyme for the hydrolysis of phosphorylated sugar lactones.

# publications[].label
Assembly of the binuclear metal center of the bacterial phosphotriesterase from Pseudomonas diminuta

# publications[].label
PhosphotriesteraseA Promising Candidate for Use in Detoxification of Organophosphates

# publications[].label
Detection of transient intermediates in the carbamoyl phosphate synthetase reaction

# publications[].label
Characterization of members of the amidohydrolase superfamily in COG3964: Determining function of Atu3266 and Ef0837

# publications[].label
N-Acetyl-D-glucosamine-6-phosphate deacetylase: substrate activation via a single divalent metal ion.

# publications[].label
Kinetic mechanism of asparagine synthetase from Vibrio cholerae.

# publications[].label
Functional Characterization of Two Polymerizing Glycosyltransferases for the Addition of N-Acetyl-d-galactosamine to the Capsular Polysaccharide of Campylobacter jejuni.

# publications[].label
Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis.

# publications[].label
Mechanism for the hydrolysis of organophosphates by the bacterial phosphotriesterase.

# publications[].label
Examination of the mechanism of sucrose synthetase by positional isotope exchange.

# publications[].label
PRIMARY AND SECONDARY O-18 ISOTOPE EFFECTS IN THE ALKALINE AND ENZYME-CATALYZED HYDROLYSIS OF PHOSPHOTRIESTERS

# publications[].label
Inactivation of organophosphorus nerve agents by the phosphotriesterase from Pseudomonas diminuta.

# publications[].label
A cysteine protease inhibitor blocks SARS-CoV-2 infection of human and monkey cells.

# publications[].label
A conserved glutamate controls the commitment to acyl-adenylate formation in asparagine synthetase.

# publications[].label
ISOTOPE EXCHANGE-REACTIONS CATALYZED BY BOVINE LIVER ARGININOSUCCINATE LYASE

# publications[].label
Expression and characterization of Escherichia coli prolidase with organophosphorus compounds

# publications[].label
A functional analysis of the allosteric nucleotide monophosphate binding site of carbamoyl phosphate synthetase.

# publications[].label
A Chemoenzymatic Synthesis of the (RP)-Isomer of the Antiviral Prodrug Remdesivir.

# publications[].label
Allosteric effects of carbamoyl phosphate synthetase from Escherichia coli are entropy-driven.

# publications[].label
Roles of hinge loop linking N- and C-terminal domains of small subunit of carbamoyl phosphate synthetase from Escherichia coli.

# publications[].label
Discovery of an L-fucono-1,5-lactonase within cog3618 from the amidohydrolase superfamily

# publications[].label
Operational control of stereoselectivity during the enzymatic hydrolysis of racemic organophosphorus compounds.

# publications[].label
THE ORGANOPHOSPHATE ACID ANHYDRASES, HYDROLYTIC ENZYMES FOR ORGANOPHOSPHATE DETOXIFICATION

# publications[].label
Quantifying the allosteric properties of Escherichia coli carbamyl phosphate synthetase: determination of thermodynamic linked-function parameters in an ordered kinetic mechanism.

# publications[].label
Regulatory changes in the control of carbamoyl phosphate synthetase induced by truncation and mutagenesis of the allosteric binding domain.

# publications[].label
Target selection and annotation for the structural genomics of the amidohydrolase and enolase superfamilies.

# publications[].label
Structure and catalytic mechanism of LigI: insight into the amidohydrolase enzymes of cog3618 and lignin degradation.

# publications[].label
ALLOSTERIC BINDING AND GLUTAMINE HYDROLYZING PROPERTIES OF ESCHERICHIA-COLI CPS

# publications[].label
Detoxification of organophosphate nerve agents by bacterial phosphotriesterase.

# publications[].label
Perforation of the tunnel wall in carbamoyl phosphate synthetase derails the passage of ammonia between sequential active sites.

# publications[].label
Enhancement, relaxation, and reversal of the stereoselectivity for phosphotriesterase by rational evolution of active site residues.

# publications[].label
Structure-based activity prediction for an enzyme of unknown function.

# publications[].label
Nanoscavenger provides long-term prophylactic protection against nerve agents in rodents.

# publications[].publisher
American Association for the Advancement of Science (AAAS)

# publications[].label
Substrate Analogues for the Enzyme-Catalyzed Detoxification of the Organophosphate Nerve Agents-Sarin, Soman, and Cyclosarin.

# publications[].label
MECHANISTIC AND KINETIC-STUDIES OF THE AMIDOTRANSFERASE ACTIVITY OF ESCHERICHIA-COLI CARBAMOYL-PHOSPHATE SYNTHETASE

# publications[].label
Tat System of Escherichia coli: Zn2+-bound Structures of tatD, ycfH and yjjV

# publications[].publisher
International Union of Crystallography (IUCr)

# publications[].label
Purification and properties of the phosphotriesterase from Pseudomonas diminuta.

# publications[].label
Interaction of bacterial luciferase with aldehyde substrates and inhibitors.

# publications[].label
The Discovery of New Enzymes in the Human Gut Microbiome

# publications[].label
KINETIC MECHANISM OF BOVINE LIVER ARGININOSUCCINATE SYNTHETASE

# publications[].label
Bacterial detoxification of organophosphate nerve agents.

# publications[].label
A Clinical-Stage Cysteine Protease Inhibitor blocks SARS-CoV-2 Infection of Human and Monkey Cells.

# publications[].label
Comparison of the functional differences for the homologous residues within the carboxy phosphate and carbamate domains of carbamoyl phosphate synthetase.

# publications[].label
Functional Annotation of Unknown Enzymes within the Amidohydrolase Superfamily

# publications[].label
From the Three-Dimensional Structure of Phosphotriesterase.

# publications[].label
Identification of the Polymerizing Glycosyltransferase Required for the Addition of d-Glucuronic Acid to the Capsular Polysaccharide of Campylobacter jejuni.

# publications[].label
Detoxification of organophosphate pesticides using a nylon based immobilized phosphotriesterase from Pseudomonas diminuta.

# publications[].label
KINETIC AND MECHANISTIC INVESTIGATION OF THE BACTERIAL LUCIFERASE REACTION

# publications[].label
PhnJ  A novel radical SAM enzyme from the CP lyase complex

# publications[].label
Functional annotation and three-dimensional structure of Dr0930 from Deinococcus radiodurans, a close relative of phosphotriesterase in the amidohydrolase superfamily.

# researcherOn[].label
Detoxification of Organophosphate Nerve Agents by Variants of Organophosphate Hydrolase

# researcherOn[].awardedBy.label
United States Department of Defense - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Enzymatic Hydrolysis of Organophosphate Esters

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# researcherOn[].label
Novel Biochemical Pathways for the Metabolism of Carbohydrates in the Human gut Micriobiome

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of General Medical Science

# researcherOn[].label
The Enzymology of Phosphonate Metabolism

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# streetAddress
Texas A&M University; Chemistry Department; 3255 TAMU

# etdChairOf[].label
Kim, Jungwook (2006-05). Molecular engineering of oligomerization and metabolite channeling through a molecular tunnel of carbamoyl phosphate synthetase. Doctoral Dissertation.

# etdChairOf[].label
Park, Min Sun (2006-10). Catalytic properties and mechanism studies of the PepQ prolidase from Escherichia coli. Master's Thesis.

# etdChairOf[].label
Kamat, Siddhesh (2012-10). Functional Annotation and Mechanistic Characterization of Enzymes with Unknown Functions: Studies on Adenine Deaminase, N-6-Methyladenine Deaminase and the C-P Lyase Pathway. Doctoral Dissertation.

# etdChairOf[].label
Nguyen, Tinh T. (2009-12). Mechanistic Insights into the Diverged Enzymes of the Amidohydrolase Superfamily. Doctoral Dissertation.

# etdChairOf[].label
Ren, Zhongjie (2016-12). Structural and Mechanistic Characterization of Carbon-Phosphorus Lyase Multi-Protein Complex and the Phosphotriesterase from Sphingobium sp. Strain TCM1. Doctoral Dissertation.

# etdChairOf[].label
Hall, Richard Stuart (2011-02). Mechanistic Studies and Function Discovery of Mononuclear Amidohydrolase Enzymes. Doctoral Dissertation.

# etdChairOf[].label
Vladimirova, Anna V (2015-05). Enzymes in COG2159 of the Amidohydrolase Superfamily: Structure and Mechanism of 5-Carboxyvanillate Decarboxylase (LIGW). Doctoral Dissertation.

# etdChairOf[].label
Cummings, Jennifer Ann (2003-05). Characterization of allantoinase from Eschericia coli. Master's Thesis.

# etdChairOf[].label
Githens, Tyler 1986- (2013-01). Improving Reactivity Against Target Organothiophosphates via Active-Site Directed Mutagenisis of a Bacterial Phosphotriesterase. Master's Thesis.

# etdChairOf[].label
Ghanem, Eman Mohamed (2006-08). Directed evolution of phosphotriesterase for detoxification of the nerve agent VX. Doctoral Dissertation.

# etdChairOf[].label
Marti Arbona, Ricardo (2006-12). Mechanistic characterization of members of the amidohydrolase superfamily. Doctoral Dissertation.

# etdChairOf[].label
Ghodge, Swapnil Vijay (2015-02). Mechanistic Characterization and Function Discovery of Phosphohydrolase Enzymes from the Amidohydrolase Superfamily. Doctoral Dissertation.

# etdChairOf[].label
Eser, Bekir Engin (2011-02). Spectroscopic and Kinetic Investigation of the Catalytic Mechanism of Tyrosine Hydroxylase. Doctoral Dissertation.

# etdChairOf[].label
Porter, Tamiko Neal (2004-12). The structure and mechanism of bacterial dihydroorotase. Doctoral Dissertation.

# etdChairOf[].label
Hitchcock, Daniel Stephen (2014-05). Functional Exploration and Characterization of the Deaminases of Cog0402. Doctoral Dissertation.

# etdChairOf[].label
Ornelas, Argentina 1982- (2012-12). Structural and Functional Characterization of Enzymes in COG3964 of the Amidohydrolase Superfamily: From Sequence to Structure to Function. Doctoral Dissertation.

# etdChairOf[].label
Ragavan, Mukundan (2014-06). Dissolution Dynamic Nuclear Polarization of Polypeptides. Doctoral Dissertation.

# etdChairOf[].label
Tripathi, Neha (2005-12). Inhibition studies of carbamoyl phosphate synthetase from Escherichia coli. Master's Thesis.

# etdChairOf[].label
Williams, LaKenya (2007-08). Analysis of the mechanisms for uronate isomerase from E. coli, cobyrinic acid a,c-diamide synthetase from S. typhimurium, and cobyric acid synthetase from S. typhimurium.. Doctoral Dissertation.

# etdChairOf[].label
Hobbs, Merlin Eric (2014-05). Mechanistic and Functional Characterization of Lactonases of COG3618 in the Amidohydrolase Superfamily. Doctoral Dissertation.

# etdChairOf[].label
Aubert, Sarah Dwyer (2004-12). Mechanism for the hydrolysis of organophosphates and investigations into the stereoselective hydrolysis of organophosphorus Esters by Phosphotriesterase.. Doctoral Dissertation.

# etdChairOf[].label
Lund, Liliya (2010-12). Biophysical and Mechanistic Characterization of Carbamoyl Phosphate Synthetase from Escherichia coli. Doctoral Dissertation.

# etdChairOf[].label
Goble, Alissa M (2013-05). Discovery of Deaminase Activities in COG1816. Doctoral Dissertation.

# etdChairOf[].label
Lum, Karin Tien (2004-09). Directed evolution of phosphotriesterase: towards the efficient detoxification of sarin and soman. Doctoral Dissertation.

# etdChairOf[].label
Cummings, Jennifer Ann (2012-02). D-Aminoacylases and Dipeptidases within the Amidohydrolase Superfamily: Relationship Between Enzyme Structure and Substrate Specificity. Doctoral Dissertation.