# overview
My laboratory studies basic biological problems using molecular genetic methods with simple microbial systems. Additionally we are developing novel microbial approaches for biotechnological applications.

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Reverse Transcriptase Polymerase Chain Reaction

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2.2 Factors Relating To The Physical Environment

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4.1 Discovery And Preclinical Testing Of Markers And Technologies

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1.1 Normal Biological Development And Functioning

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University of Chicago - (Chicago, Illinois, United States)

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Stanford University - (Stanford, California, United States)

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Draft Genome Sequence of Cupriavidus sp. Strain SK-3, a 4-Chlorobiphenyl- and 4-Chlorobenzoic Acid-Degrading Bacterium (vol 2, e00664-14, 2014)

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Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism.

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BACTERIOPHAGE SURFACE DISPLAY OF PROTEIN-A FOR ISOLATION OF BINDING VARIANTS

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Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi.

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Phosphodiesterase DosP increases persistence by reducing cAMP which reduces the signal indole.

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DNA sequence of regulatory region for integration gene of bacteriophage lambda.

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Proceedings of the National Academy of Sciences

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Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS.

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Disulfide bonds are required for Serratia marcescens nuclease activity.

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PHAGE DISPLAY OF PROTEIN-A FOR ISOLATION OF NOVEL FORMS

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Retroregulation: control of integrase expression by the b2 region of bacteriophages lambda and 434.

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Antitoxin DinJ influences the general stress response through transcript stabilizer CspE.

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Purification and characterization of 2'aminobiphenyl-2,3-diol 1,2-dioxygenase from Pseudomonas sp. LD2.

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Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide.

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Multi-copy repression of Serratia marcescens nuclease expression by dinI.

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C-terminal hybrid mutant of Bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and pH tolerance by reinforcing oligomerization.

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Nonlinear dielectric spectroscopy of live cells using superconducting quantum interference devices

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Cyanide bioremediation: the potential of engineered nitrilases.

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The integrase promoter and T'I terminator in bacteriophages lambda and 434.

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RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli.

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Microbial nitrilases: versatile, spiral forming, industrial enzymes.

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Nup124p is a nuclear pore factor of Schizosaccharomyces pombe that is important for nuclear import and activity of retrotransposon Tf1.

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The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral.

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Expression of Serratia marcescens extracellular proteins requires recA.

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Cadaverine inhibition of porin plays a role in cell survival at acidic pH.

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Arrested protein synthesis increases persister-like cell formation.

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N(2)-substituted D,L-cycloserine derivatives: synthesis and evaluation as alanine racemase inhibitors.

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Low-frequency, low-field dielectric spectroscopy of living cell suspensions

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Activation of mouse T-helper cells induces abundant preproenkephalin mRNA synthesis.

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American Association for the Advancement of Science (AAAS)

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Rapid generation of random mutant libraries.

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Differential secretion of isoforms of Serratia marcescens extracellular nuclease.

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Microbial denitrogenation of fossil fuels.

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Two-step secretion of the Serratia marcescens extracellular nuclease.

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Draft Genome Sequence of Cupriavidus sp. Strain SK-3, a 4-Chlorobiphenyl- and 4-Clorobenzoic Acid-Degrading Bacterium.

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Probing C-terminal interactions of the Pseudomonas stutzeri cyanide-degrading CynD protein.

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Probing cII and himA action at the integrase promoter pi of bacteriophage lambda.

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High efficiency transduction of single strand plasmid DNA into enteric bacteria.

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2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA.

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Sequences of the Serratia marcescens rplS and trmD genes.

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Purification and properties of 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole.

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Microbial denitrogenation of fossil fuels.

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Alanine racemase as a template for TB drug design

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Engineering pH-tolerant mutants of a cyanide dihydratase.

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Escherichia coli cryptic prophages sense nutrients to influence persister cell resuscitation.

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The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat.

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Nuclease overexpression mutants of Serratia marcescens.

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NUCLEASE OF SERRATIA-MARCESCENS - ENZYME-SUBSTRATE RATIO FOR MANIFESTING MAXIMAL ACTIVITY

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Crystallization and preliminary crystallographic analysis of a novel nuclease from Serratia marcescens.

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Residue Y70 of the Nitrilase Cyanide Dihydratase from Bacillus pumilus Is Critical for Formation and Activity of the Spiral Oligomer.

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Korean Society for Microbiology and Biotechnology

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Study of the mechanism of action of p-chloromercuribenzoate on endonuclease from the bacterium Serratia marcescens.

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Alterations in the amino-terminal third of mouse interleukin 2: effects on biological activity and immunoreactivity.

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The American Association of Immunologists

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Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan.

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Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.

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A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.

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Study of the mechanism of action of p-chloromercuribenzoate on endonuclease from the bacterium Serratia marcescens

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Regulation of the Serratia marcescens extracellular nuclease: positive control by a homolog of P2 Ogr encoded by a cryptic prophage.

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Regulatory structure of the insertion region of bacteriophage lambda.

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The MqsR/MqsA toxin/antitoxin system protects Escherichia coli during bile acid stress.

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Microbial denitrogenation of fossil fuels.

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Bacillus pumilus Cyanide Dihydratase Mutants with Higher Catalytic Activity.

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The metalloprotease gene of Serratia marcescens strain SM6.

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Escherichia coli Cryptic Prophages Sense Nutrients to Control Persister Cell Resuscitation

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[Polydispersity of Serratia marcescens nuclease at optimal pH values].

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The 1.9 A crystal structure of alanine racemase from Mycobacterium tuberculosis contains a conserved entryway into the active site.

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Draft genome sequence of the cyanide-utilizing bacterium Pseudomonas fluorescens strain NCIMB 11764.

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Mutant analysis shows that alanine racemases from Pseudomonas aeruginosa and Escherichia coli are dimeric.

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Mutational analysis of porin inhibition by polyamines

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Crystal structure at 1.45 A resolution of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms.

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Draft Genome Sequence of Cupriavidus sp. Strain SK-4, a di-ortho-Substituted Biphenyl-Utilizing Bacterium Isolated from Polychlorinated Biphenyl-Contaminated Sludge.

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Persister Cells Resuscitate Using Membrane Sensors that Activate Chemotaxis, Lower cAMP Levels, and Revive Ribosomes.

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Microbial denitrogenation of fossil fuels.

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Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD.

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Erratum for Vilo et al., Draft Genome Sequence of Cupriavidus sp. Strain SK-3, a 4-Chlorobiphenyl- and 4-Chlorobenzoic Acid-Degrading Bacterium.

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Serratia marcescens and its extracellular nuclease.

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Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.

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International Union of Crystallography (IUCr)

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Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae.

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Transposition of a beta-lactamase locus from RP1 into Pseudomonas putida degradative plasmids.

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Rapid detection of mutagens by induction of luciferase bearing prophage in Escherichia coli

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Production of active Serratia marcescens metalloprotease from Escherichia coli by alpha-hemolysin HlyB and HlyD.

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Bacterial persistence increases as environmental fitness decreases.

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The retrotransposon Tf1 possesses a specific pathway of nuclear import

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Catabolic plasmid specifying polychlorinated biphenyl degradation in Cupriavidus sp. strain SK-4: mobilization and expression in a pseudomonad.

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CynD, the cyanide dihydratase from Bacillus pumilus: gene cloning and structural studies.

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Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements.

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Characterization of the alanine racemases from two mycobacteria.

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The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli.

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Regulation of the integration-excision reaction by bacteriophage lambda.

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Genome mining of cyanide-degrading nitrilases from filamentous fungi.

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The NucE and NucD lysis proteins are not essential for secretion of the Serratia marcescens extracellular nuclease.

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Regulatory mutants and transcriptional control of the Serratia marcescens extracellular nuclease gene.

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The Serratia marcescens NucE protein functions as a holin in Escherichia coli.

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Xenogeneic silencing relies on temperature-dependent phosphorylation of the host H-NS protein in Shewanella.

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Repeated immunogenic amino acid sequences of Plasmodium species share sequence homologies with proteins from humans and human viruses.

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American Society of Tropical Medicine and Hygiene

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Evaluation of amino-substituted heterocyclic derivatives as alanine racemase inhibitors

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Characterization of a cytotoxic factor in culture filtrates of Serratia marcescens.

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Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress.

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Location of the Rz gene in bacteriophage lambda.

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Crucial elements that maintain the interactions between the regulatory TnaC peptide and the ribosome exit tunnel responsible for Trp inhibition of ribosome function.

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Gene cloning, sequence analysis, purification, and secretion by Escherichia coli of an extracellular lipase from Serratia marcescens.

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USE OF PHAGE SURFACE DISPLAY FOR PROTEIN ENGINEERING OF FUNCTIONAL PROTEINS

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Toxin YafQ increases persister cell formation by reducing indole signalling.

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A new member of the Sin3 family of corepressors is essential for cell viability and required for retroelement propagation in fission yeast.

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Disruption of polyamine modulation by a single amino acid substitution on the L3 loop of the OmpC porin channel.

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Orphan toxin OrtT (YdcX) of Escherichia coli reduces growth during the stringent response.

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Probing an Interfacial Surface in the Cyanide Dihydratase from Bacillus pumilus, A Spiral Forming Nitrilase.

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Persister Cells Resuscitate Using Membrane Sensors that Activate Chemotaxis, Lower cAMP Levels, and Revive Ribosomes

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Oligomeric structure of nitrilases: effect of mutating interfacial residues on activity.

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Effects of mutations at two neighboring aspartate residues in the L3 loop of E-coli OmpC porin.

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Genetic analysis of extracellular proteins of Serratia marcescens.

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Kinetic studies of the Serratia marcescens extracellular nuclease isoforms.

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Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

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Bacteriophage surface display of an immunoglobulin-binding domain of Staphylococcus aureus protein A.

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Antitoxin MqsA helps mediate the bacterial general stress response.

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RECOVERY OF THE GLYCEROL AUXOTROPHY OF ESCHERICHIA-COLI STRAIN BB26-36 (PLSB) BY CLONED RHODOBACTER SPHAEROIDES DNA FRAGMENT

# streetAddress
Texas A&M University; Departrment of Biology; 3258 TAMU

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Texas A&M Leadership To The Aggie Community: 'Be An Ally'

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Texas A&M Hosts Conference To Advance International Education

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Abou Nader, Mary 1983- (2012-12). Directed Evolution of Cyanide Degrading Enzymes. Doctoral Dissertation.

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Wang, Lan (2008-05). Engineering pH tolerant mutants of a cyanide dihydratase of Bacillus pumilus C1 and identifying constraints on substrate specificity in nitrilases. Master's Thesis.

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Towers, Leah Nicole (2010-12). The Mystery of the Chaetognatha: A Molecular Phylogenetic Approach Using Pelagic Chaetognath Species on Pelican Island, Galveston, Texas. Master's Thesis.

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Kumar, Krithika (2011-12). Regulation of the Nitrogen Fixation Genes in the Heterocystous Cyanobacterium Anabaena sp. Strain PCC 7120. Doctoral Dissertation.

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Ball, Timothy Keith (1990-08). Regulation of expression and excretion of a Serratia marcescens nuclease.

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Ball, Timothy Keith (1990-07). Regulation of expression and excretion of a Serratia marcescens nuclease.

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Ball, Timothy Keith (1990-09). Regulation of expression and excretion of a Serratia marcescens nuclease.

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Park, Jason M (2014-08). Regions Involved in the Oligomerization and Activity of the Spiral Forming Nitrilase Cyanide Dihydratase. Doctoral Dissertation.

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Mella Herrera, Rodrigo Andres (2010-08). Heterocyst Morphogenesis and Gene Expression in Anabaena sp. PCC 7120. Doctoral Dissertation.

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Charles, Tysheena Perkins (2008-12). Unraveling the phenotype of colicin cytoplasmic import (cim) mutants. Master's Thesis.

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Basile, Lacy Jamel (2008-08). Cyanide-degrading enzymes for bioremediation. Master's Thesis.