# positions[].organizations[].parent[].label
College of Agriculture and Life Sciences

# overview
The overall goal of my research is to understand how nitrogen (N), quantitatively the most important nutrient in crops, is managed in plants. Specifically, my research aims at how amino acids, one of the main forms of organic N in plant body, is transported. In order to study such mechanisms in detail, we have developed protein-based, fluorescent sensors that allow us to track amino acids in live cells. We utilize these sensors to discover novel molecular mechanisms that are involved in the regulation of amino acids. We are currently interrogating the processes in which amino acid exporters are involved in, using various genetic resources such as T-DNA insertion mutants and gene editing tools. We are also interested in developing novel sensors for other biologically important molecules.

# educationAndTraining[].organization.label
University of Tubingen - (Tübingen, Germany)

# publications[].label
Sponging of glutamate at the outer plasma membrane surface reveals roles for glutamate in development

# publications[].label
UMAMIT14 is an amino acid exporter involved in phloem unloading in Arabidopsis roots.

# publications[].label
Tobacco nicotine uptake permease (NUP1) affects alkaloid metabolism.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis.

# publications[].label
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering.

# publications[].label
The AAP gene family for amino acid permeases contributes to development of the cyst nematode Heterodera schachtii in roots of Arabidopsis.

# publications[].label
Glutamine Flux Imaging Using Genetically Encoded Sensors

# publications[].label
Development of a domain-specific genetic language to design Chlamydomonas reinhardtii expression vectors.

# publications[].label
Genetically encoded sensors for metabolites.

# publications[].label
Editorial: Amino Acids in Plants: Regulation and Functions in Development and Stress Defense.

# publications[].label
A novel analytical method for in vivo phosphate tracking (vol 580, pg 5885, 2006)

# publications[].label
Glutamate receptor homologs in plants: functions and evolutionary origins.

# publications[].label
Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3.

# publications[].label
Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response.

# publications[].label
Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.

# publications[].label
Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll.

# publications[].label
High Resolution Melting Temperature Analysis to IdentifyCRISPR/Cas9 Mutants from Arabidopsis.

# publications[].label
Sponging of glutamate at the outer plasma membrane surface reveals roles for glutamate in development.

# publications[].label
Imaging approach for monitoring cellular metabolites and ions using genetically encoded biosensors.

# publications[].label
Correction: Visualization of Glutamine Transporter Activities in Living Cells Using Genetically Encoded Glutamine Sensors.

# publications[].label
Quantitative imaging for discovery and assembly of the metabo-regulome.

# publications[].label
Imaging of glutamate in brain slices using FRET sensors.

# publications[].label
Circadian Patterns of the Major Nitrogen Metabolism-Related Enzymes and Metabolites in Creeping Bentgrass and the Influence of Cytokinin and Nitrate

# publications[].label
Broad-Spectrum Amino Acid Transporters ClAAP3 and ClAAP6 Expressed in Watermelon Fruits.

# publications[].label
Passive permeability controls synthesis for the allelochemical sorgeoleone in sorghum root exudate

# publications[].label
Arabidopsis UMAMIT24 and 25 are amino acid exporters involved in seed loading.

# publications[].label
Gateway-Compatible CRISPR-Cas9 Vectors and a Rapid Detection by High-Resolution Melting Curve Analysis.

# publications[].label
Quantitative imaging using genetically encoded sensors for small molecules in plants.

# publications[].label
Amino acid export in plants: a missing link in nitrogen cycling.

# publications[].label
Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Visualization of glutamine transporter activities in living cells using genetically encoded glutamine sensors.

# publications[].label
Development and use of fluorescent nanosensors for metabolite imaging in living cells.

# publications[].label
Spatiotemporal resolution of BDNF neuroprotection against glutamate excitotoxicity in cultured hippocampal neurons.

# publications[].label
High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis.

# publications[].label
Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants.

# publications[].label
Quantitative imaging approaches for small-molecule measurements using FRET sensors in plants.

# publications[].label
Glutamine flux imaging using genetically encoded sensors.

# publications[].label
Quantitative imaging approaches for small-molecule measurements using FRET sensors in plants

# publications[].label
Editorial: Amino Acids of the Glutamate Family: Functions beyond Primary Metabolism.

# publications[].label
Genetically encoded sensors for ions and metabolites

# publications[].label
A novel analytical method for in vivo phosphate tracking.

# publications[].label
Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants.

# publications[].label
MAMP-elicited changes in amino acid transport activity contribute to restricting bacterial growth.

# publications[].label
Real-time imaging of glutamate dynamics in the thalamic reticular nucleus during intrathalamic epileptiform oscillations

# publications[].label
Tissue specific expression of UMAMIT amino acid transporters in wheat.

# researcherOn[].label
Innovative Sorghum-based Production Systems with Biological Nitrification Inhibition Property to Enhance Sustainability of Agroecosystems

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.preferredLabel
USDA-National Institute of Food And Agriculture

# researcherOn[].awardedBy.label
United States Department of Defense - (Washington D.C., District of Columbia, United States)

# streetAddress
Texas A&M University; Soil & Crop Science; 2474 TAMU

# organizations[]
College of Agriculture and Life Sciences

# schools[]
College of Agriculture and Life Sciences