# positions[].organizations[].parent[].label
Texas A&M University Naresh K. Vashisht College of Medicine

# overview
The Chen Medicinal Protein Lab aims to accelerate the discovery, development and clinical translation of protein therapeutics through innovative protein engineering research.  We believe that better medicine enables a higher quality of living, and protein engineers are charged to create the better medicine for today and tomorrow. We are particularly interested in the creation and engineering of affordable protein therapeutics to prevent and treat infectious diseases and cancer.

# educationAndTraining[].organization.label
East China Normal University - (Shanghai, China)

# educationAndTraining[].organization.label
Rockefeller University - (New York, New York, United States)

# educationAndTraining[].organization.label
University of Illinois at Urbana Champaign - (Urbana, Illinois, United States)

# awardsAndHonors[].label
Faculty Early Career Development (CAREER) Program

# awardsAndHonors[].label
Faculty Early Career Development (CAREER) Program

# publications[].label
Probing the role of sigma  interaction and energetics in the catalytic efficiency of endo-1,4--xylanase.

# publications[].label
Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B.

# publications[].label
Synthesis of an intein-mediated artificial protein hydrogel.

# publications[].label
Click Detect: A Rapid and Sensitive Assay for Shiga Toxin 2 Detection.

# publications[].label
Characterization of an acid-tolerant -1,4-glucosidase from Fusarium oxysporum and its potential as an animal feed additive.

# publications[].label
A highly sensitive selection method for directed evolution of homing endonucleases.

# publications[].label
A PCR-free rapid protocol for one-pot construction of highly diverse genetic libraries.

# publications[].label
Challenges and recent advances in affinity purification of tag-free proteins.

# publications[].label
Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types.

# publications[].label
Directed evolution of homing endonuclease with altered DNA sequence specificity

# publications[].label
Methods for Engineering Binders to Multi-Pass Membrane Proteins

# publications[].label
Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027.

# publications[].label
A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin Subunits.

# publications[].label
Directed evolution of homing endonuclease I-SceI with altered sequence specificity.

# publications[].label
Directed evolution of human estrogen receptor variants with significantly enhanced androgen specificity and affinity.

# publications[].label
Directed evolution of enzymes and pathways for industrial biocatalysis.

# publications[].label
Discovery and characterization of a new cell-penetrating protein.

# publications[].label
A highly sensitive selection method for directed evolution of homing endonucleases

# publications[].label
Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation.

# publications[].label
Chemical constituents of Abies nukiangensis.

# publications[].label
Protease-stable DARPins as promising oral therapeutics.

# publications[].label
Benzhydrylpiperazine compounds inhibit cholesterol-dependent cellular entry of hepatitis C virus.

# publications[].label
A screen for genetic suppressor elements of hepatitis C virus identifies a supercharged protein inhibitor of viral replication.

# publications[].label
A simple strategy for retargeting lentiviral vectors to desired cell types via a disulfide-bond-forming protein-peptide pair.

# publications[].label
Split intein mediated ultra-rapid purification of tagless protein (SIRP).

# publications[].label
Two-component protein hydrogels assembled using an engineered disulfide-forming protein-ligand pair.

# publications[].label
PD 404,182 is a virocidal small molecule that disrupts hepatitis C virus and human immunodeficiency virus.

# publications[].label
De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against Virions.

# publications[].label
A potent and broad neutralization of SARS-CoV-2 variants of concern by DARPins.

# publications[].label
Creation and characterization of a cell-death reporter cell line for hepatitis C virus infection.

# publications[].label
Correction to: Protease-stable DARPins as promising oral therapeutics.

# publications[].label
Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Affinity Purification of Proteins in Tag-Free Form: Split Intein-Mediated Ultrarapid Purification (SIRP)

# publications[].label
A click chemistry approach to site-specific immobilization of a small laccase enables efficient direct electron transfer in a biocathode.

# publications[].label
Directed evolution of proteins for biomedical application

# publications[].label
Phenothiazines inhibit hepatitis C virus entry, likely by increasing the fluidity of cholesterol-rich membranes.

# publications[].label
A cell protection screen reveals potent inhibitors of multiple stages of the hepatitis C virus life cycle.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Evaluation of PD 404,182 as an anti-HIV and anti-herpes simplex virus microbicide.

# publications[].label
Rapid creation of a novel protein function by in vitro coevolution.

# publications[].label
A highly efficient and sensitive screening method for trans-activation activity of estrogen receptors.

# publications[].label
Repurposing an old drug: A low-cost allergy medication provides new hope for hepatitis C patients.

# publications[].label
Click display: a rapid and efficient in vitro protein display method for directed evolution.

# publications[].label
Intein-triggered artificial protein hydrogels that support the immobilization of bioactive proteins.

# publications[].label
Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification.

# publications[].label
Affinity Purification of Proteins in Tag-Free Form: Split Intein-Mediated Ultrarapid Purification (SIRP).

# publications[].label
Directed evolution of homing endonuclease with novel DNA sequence specificity

# publications[].label
In vitro incorporation of a cell-binding protein to a lentiviral vector using an engineered split intein enables targeted delivery of genetic cargo.

# researcherOn[].label
Internal Toxin Neutralizer for Treating STEC-infection

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
A Self-Assemblying Protein Hydrogel Technology for Enzyme Incorporation onto Electrodes in Biofuel Cells

# researcherOn[].awardedBy.label
United States Air Force - (Arlington, Virginia, United States)

# researcherOn[].awardedBy.preferredLabel
DOD-Air Force-Office of Scientific Research

# researcherOn[].label
A Novel Technology for Engineering Binders to Membrane Proteins

# researcherOn[].awardedBy.label
National Institute of General Medical Sciences - (Bethesda, Maryland, United States)

# researcherOn[].label
CMV Pentameric Complex Based Vaccine Strategies for Prevention of Congenital CMV

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-Eunice Kennedy Shriver National Institute of Child Health & Human Development

# researcherOn[].label
Broadly Neutralizing Non-Antibody Protein For Treating Clostridium Difficile Infection

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
Artificial Ecology Sink As Prophylaxis Against Viral Infection

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

# researcherOn[].awardedBy.preferredLabel
DHHS-NIH-National Institute of Allergy and Infectious Diseases

# researcherOn[].label
CAREER: Intein-Mediated Protein Polymer Hydrogels as General Scaffolds for Immobilization of Bioactive Proteins

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
A platform technology for the isolation of anti-cancer monoclonal antibodies from chickens

# researcherOn[].awardedBy.label
Cancer Prevention and Research Institute of Texas - (Austin, Texas, United States)

# streetAddress
Texas A&M Health Science Center; Microbial Pathogenesis And Immunology; 1114 TAMUS

# organizations[]
Texas A&M University Naresh K. Vashisht College of Medicine

# schools[]
Texas A&M University Naresh K. Vashisht College of Medicine

# etdChairOf[].label
Klemashevich, Cory (2013-05). Dihydropyridines Inhibit Translation and Early Replication of Hepatitis C Virus. Master's Thesis.

# etdChairOf[].label
Ramirez, Miguel Angel (2013-12). Intein Engineering for Protien Hydrogel Synthesis and Protein Purification. Doctoral Dissertation.

# etdChairOf[].label
Chamoun Emanuelli, Ana M (2014-08). Characterization of Viral Entry Inhibitors. Doctoral Dissertation.

# etdChairOf[].label
Simeon, Rudo Lyndon (2013-12). Selecting a path against Hepatitis C Virus. Doctoral Dissertation.