# positions[].label
Associate Professor, Food Science and Technology

# positions[].organizations[].parent[].label
College of Agriculture and Life Sciences

# overview
Dr. Castillo's research interests include the development of control measures for minimizing and reducing pathogens in fresh and fresh-cut food products, the bacterial reduction on beef and pork products and fresh produce by sanitizing rinses and the use of electron beam irradiation for food safety purposes. He has published numerous articles in peer-reviewed journals, has authored or co-authored six book chapters and has published two books as editor. He teaches the graduate course in Microbiology of Foods and co-teaches the HACCP stacked course.

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
Computational Fluid Dynamics Modeling (cfd)

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
2.2 Factors Relating To The Physical Environment

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# educationAndTraining[].organization.label
Texas A&M University - (College Station, Texas, United States)

# educationAndTraining[].organization.label
University of Guadalajara - (Guadalajara, Mexico)

# educationAndTraining[].organization.label
University of Guadalajara - (Guadalajara, Mexico)

# publications[].label
Efficacy of antimicrobials for the disinfection of pathogen contaminated green bell pepper and of consumer cleaning methods for the decontamination of knives.

# publications[].label
Development of a novel device for applying uniform doses of electron beam irradiation on carcasses.

# publications[].label
Surrogates for validation of electron beam irradiation of foods.

# publications[].label
Incidence of Vibrio cholerae in Fresh Fish and Ceviche in Guadalajara, Mexico.

# publications[].label
Letters to the editor (multiple letters)

# publications[].label
Validation of a washing and sanitizing procedure for cantaloupes at a mexican packing facility.

# publications[].label
Risk of Salmonellosis Associated with Consumption of Chocolate in Mexico.

# publications[].label
Ozone treatment for reduction of Escherichia coli 0157:H7 and Salmonella serotype typhimurium on beef carcass surfaces.

# publications[].label
Genomic Characterization of Cronobacter spp. and Salmonella spp. Strains Isolated From Powdered Infant Formula in Chile.

# publications[].label
Comparison of different washing treatments for reducing pathogens on orange surfaces and for preventing the transfer of bacterial pathogens to fresh-squeezed orange juice.

# publications[].label
JFS: Letters to the editor: Vol. 70, no. 3

# publications[].label
Reduction of Escherichia coli O157:H7 and Salmonella typhimurium on beef carcass surfaces using acidified sodium chlorite.

# publications[].label
Effect of post inoculation drying procedures on the reduction of Salmonella on almonds by thermal treatments.

# publications[].label
Polymeric Coatings with Electrolyzed Acidic Water: A Novel Approach to Extending Egg Shelf Life and Quality.

# publications[].label
Decontamination of beef carcass surface tissue by steam vacuuming alone and combined with hot water and lactic acid sprays.

# publications[].label
"Evaluation of Additional Cooking Procedures To Achieve Lethality Microbiological Performance Standards for Large, Intact Meat Products," A Comment on: J. Food Prot. 74(10):1741-1745 (2011) Response

# publications[].label
Improving ground beef safety and stabilizing color during irradiation using antioxidants, reductants or TSP.

# publications[].label
Concentrations of Escherichia coli and genetic diversity and antibiotic resistance profiling of Salmonella isolated from irrigation water, packing shed equipment, and fresh produce in Texas.

# publications[].label
Growth of Listeria monocytogenes and Listeria innocua on fresh baby spinach leaves: Effect of storage temperature and natural microflora

# publications[].label
Evaluation of peroxyacetic acid as a post-chilling intervention for control of Escherichia coli O157:H7 and Salmonella Typhimurium on beef carcass surfaces.

# publications[].label
Evaluation of peroxyacetic acid as a potential pre-grinding treatment for control of Escherichia coli O157:H7 and Salmonella Typhimurium on beef trimmings.

# publications[].label
Spread of bacterial pathogens during preparation of freshly squeezed orange juice.

# publications[].label
Survival and germination of Clostridium perfringens spores during heating and cooling of ground pork.

# publications[].label
Fabrication of Robust Superhydrophobic Coatings onto High-Density Polyethylene Food Contact Surfaces for Enhanced Microbiological Food Safety

# publications[].label
Factors Affecting Growth and Survival of Salmonella in Onion Extracts and Onion Bulbs

# publications[].label
Salmonella and Shigella in freshly squeezed orange juice, fresh oranges, and wiping cloths collected from public markets and street booths in Guadalajara, Mexico: incidence and comparison of analytical routes.

# publications[].label
Growth of Shiga toxin-producing Escherichia coli (STEC) and impacts of chilling and post-inoculation storage on STEC attachment to beef surfaces.

# publications[].label
Evaluation of additional cooking procedures to achieve lethality microbiological performance standards for large, intact meat products.

# publications[].label
The influence of surface chemistry on the kinetics and thermodynamics of bacterial adhesion.

# publications[].label
Improved multilayered antimicrobial alginate-based edible coating extends the shelf life of fresh-cut watermelon (Citrullus lanatus)

# publications[].label
Antimicrobial-Loaded Polymeric Micelles Inhibit Enteric Bacterial Pathogens on Spinach Leaf Surfaces During Multiple Simulated Pathogen Contamination Events

# publications[].label
Modification of aluminum surfaces with superhydrophobic nanotextures for enhanced food safety and hygiene

# publications[].label
Internalization of bacterial pathogens in tomatoes and their control by selected chemicals.

# publications[].label
Using antimicrobials as a food safety measure during phytosanitary treatments in mangoes

# publications[].label
Antibiotic resistance and growth of the emergent pathogen Escherichia albertii on raw ground beef stored under refrigeration, abuse, and physiological temperature.

# publications[].label
E-Beam irradiation of bagged, ready-to-eat spinach leaves (Spinacea oleracea): an engineering approach.

# publications[].label
Reduction of Salmonella and Shiga toxin-producing Escherichia coli on alfalfa seeds and sprouts using an ozone generating system.

# publications[].label
Isolation of Arcobacter spp. from retail meats and cytotoxic effects of isolates against vero cells.

# publications[].label
Chapter 11 Safety of fresh-squeezed juices

# publications[].label
The Polymyxin Ceftazidime Oxford Medium as an alternative selective and differential medium for isolation of Listeria monocytogenes from raw or unpasteurized food.

# publications[].label
Comparison of rinsing and sanitizing procedures for reducing bacterial pathogens on fresh cantaloupes and bell peppers.

# publications[].label
Comparison between the Real-Time PCR and Crystal Diagnostic Xpress Immunoassay Methods for Detecting Salmonella and Shiga Toxin-Producing Escherichia coli in the Air of Beef Slaughter Establishments.

# publications[].label
Synergistic inhibition of Listeria monocytogenes in vitro through the combination of octanoic acid and acidic calcium sulfate.

# publications[].label
Survival of salmonella transformed to express green fluorescent protein on Italian parsley as affected by processing and storage.

# publications[].label
Nanoimbibition of Essential Oils in Triblock Copolymeric Micelles as Effective Nanosanitizers against Food Pathogens Listeria monocytogenes and Escherichia coli O157:H7

# publications[].label
Alternative cooling procedures for large, intact meat products to achieve stabilization microbiological performance standards.

# publications[].label
Interventions for Hazard Control in Foods during Harvesting

# publications[].label
MONITORING OF PATHOGENIC BIOAEROSOLS IN BEEF SLAUGHTER FACILITIES BASED ON AIR SAMPLING AND AIRFLOW MODELING

# publications[].publisher
American Society of Agricultural and Biological Engineers (ASABE)

# publications[].label
Comparison of water wash, trimming, and combined hot water and lactic acid treatments for reducing bacteria of fecal origin on beef carcasses.

# publications[].label
Reduction of Bacterial Enteric Pathogens and Hygiene Indicator Bacteria on Tomato Skin Surfaces by a Polymeric Nanoparticle-Loaded Plant-Derived Antimicrobial.

# publications[].label
Simultaneous and individual quantitative estimation of Salmonella, Shigella and Listeria monocytogenes on inoculated Roma tomatoes (Lycopersicon esculentum var. Pyriforme) and Serrano peppers (Capsicum annuum) using an MPN technique.

# publications[].label
Effects of lactic acid and commercial chilling processes on survival of Salmonella, Yersinia enterocolitica, and Campylobacter coli in pork variety meats.

# publications[].label
Ecotoxic effects of paclitaxel-loaded nanotherapeutics on freshwater algae, Raphidocelis subcapitata and Chlamydomonas reinhardtii

# publications[].label
Reduction of Escherichia coli O157:H7 and Salmonella on baby spinach, using electron beam radiation.

# publications[].label
Effect of the use of a neutralizing step after antimicrobial application on microbial counts during challenge studies for orange disinfection.

# publications[].label
Survival of Vibrio cholerae O1 in ceviche and its reduction by heat pretreatment of raw ingredients.

# publications[].label
Efficacy of trimming chilled beef during fabrication to control Escherichia coli O157:H7 surrogates on subsequent subprimals.

# publications[].label
Survival and recovery of viable but noncultivable forms of Campylobacter in aqueous microcosm.

# publications[].label
Factors Affecting Growth and Survival of Salmonella in Onion Extracts and Onion Bulbs.

# publications[].label
Comparison of multiple chemical sanitizers for reducing Salmonella and Escherichia coli O157:H7 on spinach (Spinacia oleracea) leaves

# publications[].label
Inhibition of Escherichia coli O157:H7 and Salmonella enterica on spinach and identification of antimicrobial substances produced by a commercial Lactic Acid Bacteria food safety intervention.

# publications[].label
Development of durable and superhydrophobic nanodiamond coating on aluminum surfaces for improved hygiene of food contact surfaces

# publications[].label
Investigation into Formation of Lipid Hydroperoxides from Membrane Lipids in Escherichia coli O157:H7 following Exposure to Hot Water.

# publications[].label
Reduction of pathogens using hot water and lactic acid on beef trimmings

# publications[].label
Growth of Helicobacter pylori in various liquid and plating media.

# publications[].label
Shelf life and sensory characteristics of baby spinach subjected to electron beam irradiation.

# publications[].label
Use of hot water for beef carcass decontamination.

# publications[].label
Comparison of Antimicrobial Treatments Applied via Conventional or Handheld Electrostatic Spray To Reduce Shiga Toxin-Producing Escherichia coli on Chilled Beef Outside Rounds.

# publications[].label
Identification of a surrogate to validate irradiation processing of selected spices

# publications[].label
Inactivation of Salmonella and Shiga toxin-producing Escherichia coli (STEC) from the surface of alfalfa seeds and sprouts by combined antimicrobial treatments using ozone and electrolyzed water.

# publications[].label
Bacterial Hazards in Fresh and FreshCut Produce: Sources and Control

# publications[].label
Effect of air- and vacuum-packaged atmospheres on the reduction of Salmonella on almonds by electron beam irradiation

# publications[].label
Levels and enterotoxigenicity of Clostridium perfringens in pozole, tamales, and birria.

# publications[].label
Effect of electron beam irradiation on the bacterial load and sensorial quality of sliced cantaloupe

# publications[].label
Influence of Surface Roughness, Nanostructure, and Wetting on Bacterial Adhesion.

# publications[].label
Surface modification of food processing and handling gloves for enhanced food safety and hygiene

# publications[].label
Influence of Surface Roughness of Hydrophobic and Superhydrophobic Substrates on Bacterial Adhesion: A Multiscale Investigation

# publications[].label
Prevalence of Salmonella in chorizo and its survival under different storage temperatures

# publications[].label
Encapsulated Plant-Derived Antimicrobial Reduces Enteric Bacterial Pathogens on Melon Surfaces during Differing Contamination and Sanitization Treatment Scenarios

# publications[].label
Survival of Campylobacter jejuni on Sliced Watermelon and Papaya.

# publications[].label
Lactic acid sprays reduce bacterial pathogens on cold beef carcass surfaces and in subsequently produced ground beef.

# publications[].label
Chemical dehairing of bovine skin to reduce pathogenic bacteria and bacteria of fecal origin.

# publications[].label
Salmonella contamination during production of cantaloupe: a binational study.

# publications[].label
Efficacy of Traditional Almond Decontamination Treatments and Electron Beam Irradiation against Heat-Resistant Salmonella Strains.

# publications[].label
Quantitative distribution of Salmonella spp. and Escherichia coli on beef carcasses and raw beef at retail establishments.

# publications[].label
In-Plant evaluation of a lactic acid treatment for reduction of bacteria on chilled beef carcasses.

# publications[].label
Escherichia albertii Inactivation following l-Lactic Acid Exposure or Cooking in Ground Beef.

# publications[].label
Geraniol-Loaded Polymeric Nanoparticles Inhibit Enteric Pathogens on Spinach during Posttreatment Refrigerated and Temperature Abuse Storage

# publications[].label
Inhibition of Listeria monocytogenes by food antimicrobials applied singly and in combination.

# publications[].label
Attachment and Survival of Salmonella enterica and Listeria monocytogenes on Tomatoes (Solanum lycopersicum) as Affected by Relative Humidity, Temperature, and Storage Time.

# publications[].label
Effect of single and combined chemical and physical treatments on the survival of Salmonella and Escherichia coli O157:H7 attached to Valencia oranges.

# publications[].label
Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico.

# publications[].label
Reduction of Salmonella enterica serotype Poona and background microbiota on fresh-cut cantaloupe by electron beam irradiation.

# publications[].label
Purified avocado seed acetogenins: Antimicrobial spectrum and complete inhibition of Listeria monocytogenes in a refrigerated food matrix

# publications[].label
Effectiveness of a Commercial Lactic Acid Bacteria Intervention Applied to Inhibit Shiga Toxin-Producing Escherichia coli on Refrigerated Vacuum-Aged Beef.

# publications[].label
Effect of chemical sanitizers on Salmonella enterica serovar Poona on the surface of cantaloupe and pathogen contamination of internal tissues as a function of cutting procedure.

# publications[].label
Use of a novel medium, the Polymyxin Ceftazidime Oxford Medium, for isolation of Listeria monocytogenes from raw or non-pasteurized foods.

# publications[].label
Inactivation by High-Intensity Pulsed Electric Fields

# publications[].label
"Evaluation of additional cooking procedures to achieve lethality microbiological performance standards for large, intact meat products," a comment on: J. Food Prot. 74(10):1741-1745 (2011).

# publications[].label
Improving the microbiological quality and safety of fresh-cut tomatoes by low-dose electron beam irradiation.

# publications[].label
Modification of food-safe glove surfaces with superhydrophobic nanotextures for enhanced food safety

# publications[].label
Tracing Surrogates for Enteric Pathogens Inoculated on Hide through the Beef Harvesting Process.

# publications[].label
Effectiveness of potassium lactate and sodium diacetate in combination with irradiation to control Listeria monocytogenes on frankfurters.

# publications[].label
Natural Food Antimicrobials of Animal Origin

# publications[].label
Multifunctional coatings for mitigating bacterial fouling and contamination

# publications[].label
Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment

# publications[].label
Potential Control of Foodborne Pathogenic Bacteria by Pediococcus pentosaceus and Lactobacillus graminis Isolated from Fresh Vegetables

# publications[].publisher
Korean Society for Microbiology and Biotechnology

# publications[].label
Fluorescent protein-marked Escherichia coli biotype I strains as surrogates for enteric pathogens in validation of beef carcass interventions.

# researcherOn[].label
Ecology, Management and Utilization of Woody Plants on Rangelands

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Quality and Safety of Fresh-cut Vegetables and Fruits

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Roles of Produce Surface Topography and Physico-Chemistry On Development and Application of Novel Interventions To Inhibit Pathogen Attachment

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.preferredLabel
USDA-National Institute of Food And Agriculture

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
Bacteria Super-Repellent and Water-efficient, Self-Cleaning Surfaces for Vegetable Washing, Grading, and Packing Machinery and Equipment

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.preferredLabel
USDA-National Institute of Food And Agriculture

# researcherOn[].label
Cochran Fellowship Program: Ethiopia – Meat and Poultry Safety

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# streetAddress
Texas A&M University; Animal Science; 2471 TAMU

# organizations[]
College of Agriculture and Life Sciences

# schools[]
College of Agriculture and Life Sciences

# etdChairOf[].label
Adavi, Megha Sarthak (2012-07). Electron Beam Irradiation for Improving Safety of Fruits and Vegetables. Master's Thesis.

# etdChairOf[].label
Kirsch, Katie Rose (2015-08). Lactic Acid Bacteria as an Intervention Against Shiga Toxin-Producing Escherichia coli in Beef. Master's Thesis.

# etdChairOf[].label
Rodriguez, Jose Gabriel (2007-04). Development of a carcass sanitizing spray system for small and very small slaughterhouses. Master's Thesis.

# etdChairOf[].label
Arias Rios, Elba Veronica (2017-05). Standardization of Inoculation Procedures for Salmonellae and Surrogate Bacteria for Validating Spice/Herb Pathogen Reduction Processes. Doctoral Dissertation.

# etdChairOf[].label
Onafowokan, Ayoola A (2013-01). Quantitative Assessment of the Presence of Salmonella and Fecal Indicators in Mexican Tomatoes for Export to the United States. Master's Thesis.

# etdChairOf[].label
Schmidt, Heather Martin (2005-11). Improving the microbiological quality and safety of fresh-cut tomatoes by low dose electron beam irradiation. Master's Thesis.

# etdChairOf[].label
Mohammad, Zahra Hassan (2018-08). Detection of Shiga Toxin-Producing Escherichia Coli and Salmonella Aerosolized In Various Areas of Commercial Slaughter Plants by Using Dynamic Bioaerosol Monitoring Techniques. Doctoral Dissertation.

# etdChairOf[].label
Marquez Gonzalez, Mayra (2009-05). Heat resistance and outgrowth of clostridium perfringens spores as affected by the type of heating medium, and heating and cooling rates in ground pork. Doctoral Dissertation.

# etdChairOf[].label
Neal, Jack A. (2010-07). THE EFFECTS OF ELECTRON BEAM IRRADIATION AND SANITIZERS IN THE REDUCTION OF PATHOGENS AND ATTACHMENT PREVENTION ON SPINACH. Doctoral Dissertation.

# etdChairOf[].label
Villarreal Silva, Mariana (2011-10). Simulation of Contamination Through the Post-Harvest Environment Using Surrogate Organisms. Master's Thesis.

# etdChairOf[].label
Cuervo Pliego, Mary (2012-02). Comparison of Current Almond Pasteurization Methods and Electron Beam Irradiation as an Alternative. Doctoral Dissertation.

# etdChairOf[].label
Villarreal Silva, Mariana (2016-08). Role of Epiphytic Bacteria in the Colonization of Fruits and Leafy Greens by Foodborne Bacterial Pathogens. Doctoral Dissertation.

# etdChairOf[].label
Cuervo Pliego, Mary Pia (2009-05). Effect of natural antimicrobials against Salmonella, Escherichia coli o157:h7 and Listeria monocytogenes. Master's Thesis.

# etdChairOf[].label
Kakani, Grihalakshmi (2009-05). Interventions for ensuring food safety in mangoes during phytosanitary treatments. Master's Thesis.

# etdChairOf[].label
Perez, Keila Lizth (2011-08). Efficacy of Consumer-Available Antimicrobials for the Disinfection of Pathogen Contaminated Green Bell Pepper and Efficacy of Consumer Cleaning Methods for the Decontamination of Knives. Master's Thesis.

# etdChairOf[].label
Yegin, Yagmur (2020-11). Mechanistic Investigation of Inactivating Bacterial Food Pathogens with Nanoparticles. Doctoral Dissertation.

# etdChairOf[].label
Mohammed, Zahra Hassan (2013-08). Effect of Accelerated Drying on the Reduction of Salmonella on Almonds by Thermal and Electron Beam Irradiation Pasteurization Treatments. Master's Thesis.

# etdChairOf[].label
Vadlamudi, Sasi (2005-02). Effect of sanitizer treatments on Salmonella enterica serotype Poona on the surface of cantaloupe and cell transfer to the internal tissue during cutting practices. Master's Thesis.

# etdChairOf[].label
Hudson, Jessica C. (2015-12). Comparison of Antimicrobial Treatments Applied via Conventional Spray or Electrostatic Spray to Reduce Shiga-Toxigenic Escherichia coli (STEC) on Chilled Beef Outside Rounds. Master's Thesis.

# etdChairOf[].label
Bryant, Alana Michelle (2017-11). Factors Affecting the Growth and Survival of Salmonella and Shiga Toxin-Producing Escherichia coli on Microgreens. Doctoral Dissertation.