# overview
Dr Svoboda is a well-established senior principal investigator with a broad background in developmental biology and cellular biology. Her research focus is on the cell biology of whole embryonic tissues, including cornea, cartilage, palate. Her lab has been funded from NIH, March of Dimes, Foundations and Private Companies for 3 decades. As a postdoctoral fellow at Harvard Medical School, she carried out cell and molecular biology experiments on developing systems and worked with Dr. Elizabeth Hay when she developed her theories on cell-matrix interactions. As PI or co-Investigator on many previous university- and March of Dimes funded grants (over 30 years of continuous funding), she worked on how cell-matrix interactions change during development. In addition, she was a mentor on two training grants (T32 and KL2) and has successfully administered other NIH supported developmental and cell biology projects (e.g. staffing, research protections, and budget), collaborated with other researchers, and produced peer-reviewed publications from each project.

She has a new project that contributes evidence to the theory that periocular mesenchyme (POM) cells contribute to the development of the ciliary body, trabecular meshwork and the iridocorneal angle. The objective of this project is to determine if Gli1 positive cells contribute to the POM and anterior eye structures by using inducible Gli1-CreERT2; tdTomatoflox (Gli1-tdTomato) mouse model. Experiments were recently completed that demonstrated the Gli1 + cells were also positive for Pitx2, FOXC1, and FOXC2, known markers for periocular mesenchyme during anterior eye development.

She has successfully trained 40 Postdoctoral, Ph.D., M.S. graduate students, undergraduate, medical and dental predoctoral students, and college/high school summer research trainees.

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1.1 Normal Biological Development And Functioning

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1.1 Normal Biological Development And Functioning

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Reverse Transcriptase Polymerase Chain Reaction

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1.1 Normal Biological Development And Functioning

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3214 Pharmacology And Pharmaceutical Sciences

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Reverse Transcriptase Polymerase Chain Reaction

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1.1 Normal Biological Development And Functioning

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1.1 Normal Biological Development And Functioning

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Reverse Transcriptase Polymerase Chain Reaction

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Receptor Activator Of Nuclear Factor-kappa B

# educationAndTraining[].organization.label
University of Nebraska Medical Center - (Omaha, Nebraska, United States)

# educationAndTraining[].organization.label
University of Nebraska at Omaha - (Omaha, Nebraska, United States)

# educationAndTraining[].organization.label
University of Nebraska at Omaha - (Omaha, Nebraska, United States)

# publications[].label
An analysis of cell shape and the neuroepithelial basal lamina during optic vesicle formation in the mouse embryo.

# publications[].label
The NH2-terminal and COOH-terminal fragments of dentin matrix protein 1 (DMP1) localize differently in the compartments of dentin and growth plate of bone.

# publications[].label
Increased cell diameter precedes chondrocyte terminal differentiation, whereas cell-matrix attachment complex proteins appear constant.

# publications[].label
The ''cell-matrix attachment complex'' (CMAX) in embryonic epithelia contains zyxin and vinculin.

# publications[].label
The pros and cons of apoptosis assays for use in the study of cells, tissues, and organs.

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Cellular invasion and collagen type IX in the primary corneal stroma in vitro.

# publications[].label
Antioxidants counteract nicotine and promote migration via RacGTP in oral fibroblast cells.

# publications[].label
ECM-stimulated actin bundle formation in embryonic corneal epithelia is tyrosine phosphorylation dependent

# publications[].label
Comparison of Vibringe, EndoActivator, and needle irrigation on sealer penetration in extracted human teeth.

# publications[].label
ECM-stimulated actin bundle formation in embryonic corneal epithelia is tyrosine phosphorylation dependent.

# publications[].label
Oral Sciences PhD Program Enrollment, Graduates, and Placement: 1994 to 2016.

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Distinct compartmentalization of dentin matrix protein 1 fragments in mineralized tissues and cells.

# publications[].label
Effect of ADAM17 on EMMPRIN Expression after Vesicant Injury to the Cornea

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Effects of hydrogen peroxide on dental unit biofilms and treatment water contamination.

# publications[].label
The intracellular distribution of vinculin and alpha 2 integrin in epithelial cells and chondrocytes.

# publications[].label
THE EFFECTS OF VINCRISTINE SULFATE ON MOUSE EMBRYO GROWTH, DEVELOPMENT, AND OPTIC VESICLE FORMATION

# publications[].label
Histology of NeoMTA Plus and Quick-Set2 in Contact with Pulp and Periradicular Tissues in a Canine Model.

# publications[].label
Increased cell diameter precedes chondrocyte terminal differentiation, whereas cell-matrix attachment complex proteins appear constant

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Embryonic chick corneal epithelium: a model system for exploring cell-matrix interactions.

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Ephrin reverse signaling mediates palatal fusion and epithelial-to-mesenchymal transition independently of Tgf3.

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Antioxidant combinations protect oral fibroblasts against metal-induced toxicity.

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Selected abstracts of the Seventeenth Annual Meeting of the East Coast Connective Tissue Society

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Vacuum-induced suction stimulates increased numbers of blood vessels in healthy dog gingiva

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Sheets of embryonic epidermis can be isolated and cultured with or without the basal lamina.

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Vacuum-induced Suction Stimulates Increased Numbers of Blood Vessels in Healthy Dog Gingiva.

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Digital vs Paper Histology Lab Exams: Pros and Cons

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Effects of estrogen on chondrocyte proliferation and collagen synthesis in skeletally mature articular cartilage.

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Nicotine inhibits human gingival fibroblast migration via modulation of Rac signalling pathways.

# publications[].label
Establishment of a whole-chick sternum model that recapitulates normal cartilage development.

# publications[].label
Cleft lip and palate genetics and application in early embryological development.

# publications[].label
ADAM17 Inhibitors Attenuate Corneal Epithelial Detachment Induced by Mustard Exposure.

# publications[].publisher
Association for Research in Vision and Ophthalmology (ARVO)

# publications[].label
Reactive oxygen species and antioxidant defense mechanisms in the oral cavity: a literature review.

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Characterization of Bsk mice: I. The Bsk mutation does not involve a recombination of cornea-specific keratin 12 and skin-specific hair keratin genes.

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Rho is present and phosphorylated in avian corneal epithelia cultured in the presence of ECM molecules.

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Macrophage morphology and migration are influenced by ECM substrate

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SM1997 downregulates mustard-induced enzymes that disrupt corneal epithelial attachment.

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INTRACELLULAR LABELING OF BETA-ACTIN MESSENGER-RNA USING REVERSE-TRANSCRIPTASE INCORPORATED BIOTIN-DUTP INTO THE ACTIN CORTICAL MAT OF CORNEAL EPITHELIAL-CELLS

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Reactive oxygen species and antioxidant defense mechanisms in the oral cavity: a literature review.

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Evaluation of an automated dental unit water system's contamination control protocol.

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Growth Factors and Signaling Proteins in Craniofacial Development

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Erratum: Establishment of a whole-chick sternum model that recapitulates normal cartilage development (Biotechniques (April 1998) 24 (632-636))

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Embryonic corneal epithelial interaction with exogenous laminin and basal lamina is F-actin dependent.

# publications[].label
Extracellular matrix protein expression in terminally differentiating chondrocytes is integrin mediated.

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Upregulation of gamma-2 laminin-332 in the mouse ear vesicant wound model.

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Anti-inflammatory protein tumor necrosis factor--stimulated protein 6 (TSG-6) promotes early gingival wound healing: an in vivo study.

# publications[].label
Approaches to studying cellular signaling: a primer for morphologists.

# publications[].label
Tensin, paxillin and focal adhesion kinase may be involved in actin reorganization stimulated by ECM in corneal epithelia

# publications[].label
Chondrocyte survival and differentiation in situ are integrin mediated

# publications[].label
Zyxin and vinculin distribution at the cell-extracellular matrix attachment complex (CMAX) in corneal epithelial tissue are actin dependent.

# publications[].label
Cell-Matrix Interactions and Signal Transduction

# publications[].label
Distribution of F-actin, vinculin and integrin subunits (alpha 6 and beta 4) in response to corneal substrata.

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Diesel exhaust particle exposure causes redistribution of endothelial tube VE-cadherin.

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Map kinase and PI3 kinase are downstream of Rho and Fak in the integrin mediated signaling cascade necessary for ACM reorganization in avian corneal epithelia

# publications[].label
Precursors of macrophages in embryonic rat lungs fail to exhibit granulocyte-forming potential.

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Chondrocyte terminal differentiation, apoptosis, and type X collagen expression are downregulated by parathyroid hormone.

# publications[].label
Bioactive antioxidant mixtures promote proliferation and migration on human oral fibroblasts.

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Comparison and modulation of the Rho mediated signaling pathways in dermal and embryonic corneal fibroblasts.

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The molecules in the corneal basement membrane zone affected by mustard exposure suggest potential therapies.

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Doxycycline hydrogels as a potential therapy for ocular vesicant injury.

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THE EFFECTS OF VINCRISTINE SULFATE ON OPTIC VESICLE FORMATION IN THE MOUSE EMBRYO

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Intracellular localization of types I and II collagen mRNA and endoplasmic reticulum in embryonic corneal epithelia.

# publications[].label
The role of twist during palate development.

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The biological function of type I receptors of bone morphogenetic protein in bone.

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Expression of metalloproteinases and their inhibitors change when sternal cartilage is organ cultured with integrin antibodies.

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Cell-matrix and cell-cell interactions of human gingival fibroblasts on three-dimensional nanofibrous gelatin scaffolds.

# publications[].label
Extracellular matrix: The proteins that function throughout the body.

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Treatment of gum diseases and gum disorders with TSG-6 protein

# publications[].label
Embryonic corneal epithelial actin alters distribution in response to laminin.

# publications[].label
Signal transduction pathways involved in epithelial-mesenchymal transition in oral cancer compared with other cancers.

# publications[].label
Nicotine inhibits myofibroblast differentiation in human gingival fibroblasts.

# publications[].label
TGF Signaling Regulates Decorin and Biglycan Expression and Distribution During Murine Palatal Fusion

# publications[].label
How may paths exist from the membrane to the nucleus?

# publications[].label
Regulation of epithelial-mesenchymal transition in palatal fusion.

# publications[].label
Bioactive polyphenol antioxidants protect oral fibroblasts from ROS-inducing agents.

# publications[].label
Analysis of Snail1 function and regulation by Twist1 in palatal fusion.

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THE INTRACELLULAR-DISTRIBUTION OF VINCULIN AND ALPHA(2)-INTEGRIN IN EPITHELIAL-CELLS AND CHONDROCYTES

# publications[].label
Parathyroid hormone (PTH) inhibits terminal chondrocyte differentiation and type X collagen production in cultured whole chick sterna.

# publications[].label
Chondrocyte survival and differentiation in situ are integrin mediated.

# publications[].label
PI-3 kinase activity is required for epithelial-mesenchymal transformation during palate fusion.

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Embryonic chicken cornea and cartilage synthesize type IX collagen molecules with different amino-terminal domains.

# publications[].publisher
Proceedings of the National Academy of Sciences

# publications[].label
Hyperbaric oxygen downregulates ICAM-1 expression induced by hypoxia and hypoglycemia: the role of NOS.

# publications[].label
Cell polarization: From epithelial cells to odontoblasts.

# publications[].label
Nicotine inhibits palatal fusion and modulates nicotinic receptors and the PI3 kinase pathway in medial edge epithelia*

# publications[].label
Ephrin reverse signaling controls palate fusion via a PI3 kinase-dependent mechanism.

# publications[].label
CONFOCAL MICROSCOPY OF WHOLE-MOUNT EMBRYONIC CARTILAGE - INTRACELLULAR-LOCALIZATION OF F-ACTIN, CHICK PROLYL HYDROXYLASE AND TYPE-II COLLAGEN MESSENGER-RNA

# publications[].label
Optic vesicle defects induced by vincristine sulfate: an in vivo and in vitro study in the mouse embryo.

# publications[].label
Modulation of the Rho mediated signaling pathways in embryonic corneal fibroblasts

# publications[].label
Intracellular distribution of type II collagen mRNA and prolyl 4-hydroxylase in embryonic avian corneal epithelia.

# publications[].label
Parathyroid hormone/parathyroid hormone-related peptide modulates growth of avian sternal cartilage via chondrocytic proliferation

# publications[].label
A tribute to Elizabeth D. Hay, 1927-2007.

# publications[].label
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent.

# publications[].label
Intracellular distribution of beta-actin mRNA is polarized in embryonic corneal epithelia.

# publications[].label
Beta 1 integrin antibodies inhibit chondrocyte terminal differentiation in whole sterna.

# publications[].label
Method of Studying Palatal Fusion using Static Organ Culture.

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Cell fate mediators Notch and Twist in mouse mandibular condylar cartilage.

# publications[].label
Extracellular Matrix in Secondary Palate Development.

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Timed Induction of Gli1 (P3 To P30) Was Expressed In Mouse Trabecular Meshwork

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ROCK inhibitor (Y27632) increases apoptosis and disrupts the actin cortical mat in embryonic avian corneal epithelium.

# publications[].label
An in vivo comparison of wound healing characteristics of two commercial acellular dermal matrices.

# publications[].label
Biglycan and Decorin Expression and Distribution in Palatal Adhesion.

# publications[].label
Chondrocyte survival and differentiation in situ are integrin mediated

# publications[].label
Expression of Collagen Types I, II, IX, and X in the Mineralizing Turkey Gastrocnemius Tendon.

# publications[].label
Comparative studies of biorad and leica confocal laser scanning microscopes

# publications[].label
PTH stimulated growth and decreased Col-X deposition are phosphotidylinositol-3,4,5 triphosphate kinase and mitogen activating protein kinase dependent in avian sterna.

# publications[].label
Parathyroid hormone/parathyroid hormone-related peptide modulates growth of avian sternal cartilage via chondrocytic proliferation.

# publications[].label
Activation of Receptor Activator of Nuclear Factor-B Ligand and Matrix Metalloproteinase Production in Periodontal Fibroblasts by Endothelin Signaling.

# publications[].label
THE TERATOGENETIC EFFECTS OF VINCRISTINE SULFATE ON GROWTH, DEVELOPMENT, AND OPTIC VESICLE FORMATION IN THE EARLY SOMITE MOUSE EMBRYO

# publications[].label
ECM stimulated reorganization of the actin cortical mat in epithelial tissues may involve tensin, paxillin and focal adhesion kinase

# publications[].label
Zyxin and vinculin distribution at the cell-extracellular matrix attachment complex (CMAX) in corneal epithelial tissue are actin dependent

# publications[].label
Erk and PI-3 kinase are necessary for collagen binding and actin reorganization in corneal epithelia.

# publications[].label
Periocular Mesenchyme (POM) Contributes to Anterior Eye Structures

# publications[].label
Sulfur mustard induces an endoplasmic reticulum stress response in the mouse ear vesicant model.

# publications[].label
Intracellular relationship between actin and alpha-actinin in a whole corneal epithelial tissue.

# publications[].label
Collagen expression and orientation in ocular tissues

# researcherOn[].label
Mechanism of Ephrin Signaling in Mammalian Palatal Fusion

# researcherOn[].awardedBy.label
National Institutes of Health - (Bethesda, Maryland, United States)

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DHHS-NIH-National Institute of Dental and Craniofacial Research

# streetAddress
3302 Gaston Ave; Biomedical Sciences; 3302 Gaston Avenue

# etdChairOf[].label
Couto, Sophie Rose (2020-03). A COMPARATIVE STUDY OF WOUND HEALING OF TWO COMMERCIAL ACELLULAR DERMAL MATRICES: AN IN VIVO STUDY. Master's Thesis.

# etdChairOf[].label
Alshehri, Wedad (2020-07). Effect of Silver Diamine Fluoride on the Treatment of Gingivitis in Geriatric Patients. Doctoral Dissertation.

# etdChairOf[].label
Ibrahim, Isra (2015-04). Decorin and Biglycan Expression and Distribution during Palatal Shelf Adhesion. Master's Thesis.

# etdChairOf[].label
Serrano, Maria Juliana (2015-04). EPH and Ephrins in Palate Development. Doctoral Dissertation.

# etdChairOf[].label
Beltran, Stacy Renay (2014-04). Anti-inflammatory Protein TSG-6 Promotes Early Gingival Wound Healing: An In Vivo Study. Master's Thesis.

# etdChairOf[].label
Richert, Jordan Chay (2016-04). A Comparison of Rat Gingival Fibroblast Attachment on Commercial Acellular Dermal Matrices: An In Vitro Study. Master's Thesis.