# positions[].organizations[].parent[].label
Texas A&M Engineering Experiment Station (TEES)

# positions[].organizations[].parent[].label
College of Agriculture and Life Sciences

# overview
Nikolov's Bioseparations Lab conducts transformative research in bioprocess engineering aimed at the development of novel and cost-effective strategies for extraction and purification of recombinant and native biomolecules. Bioseparations Lab leverages scientific and engineering expertise of lab members to find solutions for a variety of bioprocessing and separations challenges that currently face plant and algal biotechnology. To guide early process development and identify constraints posed by biological system and final product lab members use process simulation. Past and current research projects directed by Dr. Nikolov include industrial protein products derived from rice, sugarcane, tobacco, Lemna minor, and microalgae.

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# keywords[]
Reverse Transcriptase Polymerase Chain Reaction

# keywords[]
30 Agricultural, Veterinary And Food Sciences

# educationAndTraining[].organization.label
University of Novi Sad - (Novi Sad, Serbia)

# educationAndTraining[].organization.label
Iowa State University - (Ames, Iowa, United States)

# educationAndTraining[].organization.label
Iowa State University - (Ames, Iowa, United States)

# publications[].label
A METHOD FOR THE DETERMINATION OF ALPHA-DICARBONYL COMPOUNDS

# publications[].label
CHARACTERIZATION OF GLUCOAMYLASE ADSORPTION TO RAW STARCH

# publications[].label
Factors influencing recombinant human lysozyme extraction and cation exchange adsorption.

# publications[].label
RECOVERY OF LACTIC-ACID BY SORPTION - RESIN EVALUATION

# publications[].label
Considerations for the recovery of recombinant proteins from plants.

# publications[].label
Aqueous Fractionation of Dry-Milled Corn Germ for Food Protein Production

# publications[].label
Process development of enzymatically-generated algal protein hydrolysates for specialty food applications

# publications[].label
Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system.

# publications[].label
BIOT 251-Host system choice challenges bioprocessing of plant-derived therapeutics

# publications[].label
Review of the harvesting and extraction program within the National Alliance for Advanced Biofuels and Bioproducts

# publications[].label
Evaluation of alternatives for human lysozyme purification from transgenic rice: impact of phytic acid and buffer.

# publications[].label
RETENTION OF CARBOHYDRATES ON SILICA AND AMINE-BONDED SILICA STATIONARY PHASES - APPLICATION OF THE HYDRATION MODEL

# publications[].label
Downstream Processing of Transgenic Plant Systems: Protein Recovery and Purification Strategies

# publications[].label
Supercritical fluid extraction of black pepper (Piper nigrun L.) essential oil

# publications[].label
SOME ANALYTICAL ASPECTS OF ENZYME DEGRADATION OF STARCH AND INULIN INTO MALTOOLIGOSACCHARIDE AND FRUCTOOLIGOSACCHARIDE

# publications[].label
A glutathione S-transferase fusion protein with the starch-binding domain of Aspergillus glucoamylase.

# publications[].label
OFF-FLAVOR REMOVAL FROM SOY-PROTEIN ISOLATE BY USING LIQUID AND SUPERCRITICAL CARBON-DIOXIDE

# publications[].label
ACCELERATED DEGRADATION STUDIES OF STARCH-FILLED POLYETHYLENE FILMS

# publications[].label
Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas reinhardtii

# publications[].label
Harvesting Nannochloris oculata by inorganic electrolyte flocculation: effect of initial cell density, ionic strength, coagulant dosage, and media pH.

# publications[].label
Expression and recovery of a recombinant protein from transgenic maize.

# publications[].label
Effects of mutations in the starch-binding domain of Bacillus macerans cyclodextrin glycosyltransferase.

# publications[].label
Selective extraction of carotenoids and proteins from Chlorella vulgaris

# publications[].publisher
American Society of Agricultural and Biological Engineers (ASABE)

# publications[].label
BIOT 267-Pretreatment of plant extracts containing MAb may be desirable

# publications[].label
Evaluation of monoclonal antibody and phenolic extraction from transgenic Lemna for purification process development.

# publications[].label
Potential Applications for Supercritical Carbon Dioxide Separations in Soybean Processing

# publications[].label
Purification of Monoclonal Antibodies From Plants

# publications[].label
SOLUBILITY OF FATTY-ACIDS IN SUPERCRITICAL CARBON-DIOXIDE

# publications[].label
Aqueous extraction of beta-glucuronidase from transgenic canola: kinetics and microstructure.

# publications[].label
Production and recovery of multimeric malaria antigen (Pfs 25) complexes from Chlamydomonas reinhardtii chloroplast

# publications[].label
EVALUATING STARCH-PROTEIN INTERACTIONS FOR RECOVERY OF RECOMBINANT PROTEINS

# publications[].label
CHARACTERIZATION OF A BETA-GALACTOSIDASE FUSION PROTEIN CONTAINING THE STARCH-BINDING DOMAIN OF ASPERGILLUS GLUCOAMYLASE

# publications[].label
Making therapeutic proteins in transgenic corn

# publications[].label
Processing of permeabilized Chlorella vulgaris biomass into lutein and protein-rich products

# publications[].label
EFFECT OF COMPOUNDING AND STARCH MODIFICATION ON PROPERTIES OF STARCH-FILLED LOW-DENSITY POLYETHYLENE

# publications[].label
Improved adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding domain from Aspergillus glucoamylase.

# publications[].label
Enhanced Expression Levels of Cellulase Enzymes Using Multiple Transcription Units

# publications[].label
Commercial opportunities and challenges for protein products from corn

# publications[].label
CHEMICAL METHOD FOR THE DETERMINATION OF STARCH IN POLYETHYLENE

# publications[].label
Effect of phytic acid and buffer ions on recombinant human lysozyme purification

# publications[].label
Purification and characterization of recombinant Cel7A from maize seed.

# publications[].label
Production and purification of two recombinant proteins from transgenic corn.

# publications[].label
KINETICS AND EQUILIBRIA OF REVERSION REACTIONS CATALYZED BY GLUCOAMYLASE

# publications[].label
Functional starch-binding domain of Aspergillus glucoamylase I in Escherichia coli.

# publications[].label
Processing considerations for use of canola for recombinant protein production.

# publications[].label
Processing of transgenic corn seed and its effect on the recovery of recombinant beta-glucuronidase.

# publications[].label
Recovery and purification of plant-made recombinant proteins.

# publications[].label
Purification of recombinant aprotinin produced in transgenic corn seed: Separation from CTI utilizing ion-exchange chromatography

# publications[].label
Production of recombinant proteins in transgenic plants: Practical considerations

# publications[].label
Process for selective extraction of pigments and functional proteins from Chlorella vulgaris

# publications[].label
Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality.

# publications[].label
Production of human monoclonal antibody in eggs of chimeric chickens.

# publications[].label
BIOT 211-Evaluation and removal of key plant-derived impurities during downstream process development

# publications[].label
DISACCHARIDE PRODUCTION BY GLUCOAMYLASE IN AQUEOUS ETHER MIXTURES

# publications[].label
Fractionation of transgenic corn for recovery of recombinant enzymes

# publications[].label
Kinetics, equilibria, and modeling of the formation of oligosaccharides from D‐glucose with Aspergillus niger glucoamylases I and II

# publications[].label
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates.

# publications[].label
Production of recombinant proteins in transgenic plants: Practical considerations.

# publications[].label
Impact of Dry-milled Germ Processing on Aqueous Protein and Oil Extraction

# publications[].label
Domain E of Bacillus macerans cyclodextrin glucanotransferase: An independent starch-binding domain.

# publications[].label
Degradation of Degradable Starch-Polyethylene Plastics in a Compost Environment

# publications[].label
Extraction and fractionation of microalgae-based protein products

# publications[].label
Effect of processing on the recovery of recombinant beta-glucuronidase (rGUS) from transgenic canola.

# publications[].label
BIOSPECIFIC ADSORPTION OF GLUCOAMYLASE-I FROM ASPERGILLUS-NIGER ON RAW STARCH

# publications[].label
Effect of algogenic organic matter (AOM) and sodium chloride on Nannochloropsis salina flocculation efficiency.

# publications[].label
2000 Congress Symposium on Molecular Farming: Development of an edible subunit vaccine in corn against enterotoxigenic strains of Escherichia coli

# publications[].label
Characterization and application of porcine liver aldehyde oxidase in the off-flavor reduction of soy proteins

# publications[].label
Corn protein & oil recovery after germ wet milling

# publications[].label
Processing of transgenic corn seed and its effect on the recovery of recombinant -glucuronidase

# publications[].label
Downstream processing of recombinant proteins from transgenic feedstock.

# publications[].label
BETA-GALACTOSIDASE FUSION PROTEINS WITH THE STARCH-BINDING DOMAINS OF GLUCOAMYLASE-I AND CYCLODEXTRIN GLUCANOTRANSFERASE

# publications[].label
Expression and recovery of immunotoxins from Chlamydomonas reinhardtii chloroplast

# publications[].label
Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii.

# publications[].label
Recombinant aprotinin produced in transgenic corn seed: Extraction and purification studies

# publications[].label
Evaluation of processing options for transgenic sugarcane tissue expressing bovine lysozyme

# publications[].label
Subsite mapping of Aspergillus niger glucoamylases I and II with malto‐ and isomaltooligosaccharides

# publications[].label
Evaluation of pretreatment methods for primary recovery and capture of an antibody fragment (alpha CD22scFv) from Chlamydomonas reinhardtii lysates

# publications[].label
Phenolics removal from transgenic Lemna minor extracts expressing mAb and impact on mAb production cost.

# publications[].label
Transformation of glycerol and cellulosic materials into high energy fuels

# publications[].label
Recovery and Purification of Lactic Acid from Fermentation Broth by Adsorption

# publications[].label
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF OLIGOSACCHARIDES USING AMINE MODIFIED SILICA COLUMNS

# publications[].label
Starch-binding domain of Aspergillus glucoamylase-I. Interaction with beta-cyclodextrin and maltoheptaose.

# publications[].label
Recovery and purification of lactic acid from fermentation broth by adsorption

# publications[].label
Purification of Antibodies From Transgenic Plants

# publications[].label
Process evaluations and economic analyses of recombinant human lysozyme and hen egg‐white lysozyme purifications

# publications[].label
Process and economic evaluation of the extraction and purification of recombinant beta-glucuronidase from transgenic corn.

# publications[].label
Recovery of bovine lysozyme from transgenic sugarcane stalks: extraction, membrane filtration, and purification.

# publications[].label
Removal of phenolic compounds by adsorption on polymeric resins before protein purification

# publications[].label
Capture chromatography with mixed-mode resins: A case study with recombinant human thioredoxin from Escherichia coli.

# researcherOn[].label
UNS: Collaborative Research: Nanoengineering Biomimetic Nanobrushes for Pathogen Sensing

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Use of Algae in the Production of Biofuels - Bulgaria

# researcherOn[].awardedBy.label
United States Department of Agriculture - (Washington D.C., District of Columbia, United States)

# researcherOn[].label
FFATA: Collaborative Research:  Development  of an Integrated Algae Platform for Affordable Protein Therapeutic and Vaccine Molecules

# researcherOn[].awardedBy.label
National Science Foundation - (Arlington, Virginia, United States)

# researcherOn[].label
Sustainable bioprocesses for recovery of high-value protein products from plants and microalgae

# researcherOn[].awardedBy.label
National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States)

# streetAddress
Texas A&M University; Biological & Agricultural Engineering; 2117 TAMU

# schools[]
Texas A&M Engineering Experiment Station (TEES)

# schools[]
College of Agriculture and Life Sciences

# inTheNews[].label
US researchers develop Covid-19 spike proteins to identify antibodies

# etdChairOf[].label
Imam, Tahmina (2010-01). SEPARATION OF PROTEINS BY ION EXCHANGE AND MEMBRANE CHROMATOGRAPHY: BUFFER COMPOSITION, INTERFERING IMPURITIES AND FOULING CONSIDERATIONS. Master's Thesis.

# etdChairOf[].label
Ravi, Ayswarya (2019-07). EVALUATION OF MIXED-MODE CHROMATOGRAPHY RESINS FOR ISOLATION OF RECOMBINANT THERAPEUTIC PROTEINS. Doctoral Dissertation.

# etdChairOf[].label
Munjal, Neera (2014-12). Bioprocessing of Microalgae C. reinhardtii for Production and Purification of Single Chain Antibody Fragment. Master's Thesis.

# etdChairOf[].label
Kulkarni, Sayali Vishwas (2018-08). Bioprocessing of Microalgae for Extraction of High-Value Products. Doctoral Dissertation.

# etdChairOf[].label
Quinones, Katelyn (2015-08). Bioprocessing of Recombinant Proteins Produced in the Chloroplast of Chlamydomonas reinhardtii. Master's Thesis.

# etdChairOf[].label
Wilken, Lisa Rachelle (2012-02). Recovery of Recombinant and Native Proteins from Rice and Corn Seed. Doctoral Dissertation.

# etdChairOf[].label
Guo, Shengchun (2018-05). Bioprocess Development for Recombinant Therapeutic Protein Osteopontin from Escherichia Coli. Master's Thesis.

# etdChairOf[].label
Johnson, Jecori Shana (2018-04). Selective Separation of Proteins Using Stimuli-Responsive Polymers in an Aqueous System. Master's Thesis.

# etdChairOf[].label
Garzon Sanabria, Andrea J (2013-07). Bioprocessing of Microalgae for Bioenergy and Recombinant Protein Production. Doctoral Dissertation.

# etdChairOf[].label
Davis, Ryan T. (2012-02). Characterizing Microalgae (Nannochloris oculata) Harvesting by Aluminum Flocculation. Master's Thesis.

# etdChairOf[].label
Barros, Georgia (2012-07). Downstream Processing of Recombinant Proteins from Transgenic Plant Systems: Phenolic Compounds Removal from Monoclonal Antibody Expressing Lemna minor and Purification of Recombinant Bovine Lysozyme from Sugarcane. Doctoral Dissertation.

# etdChairOf[].label
Kulkarni, Sayali Vishwas (2018-06). Bioprocessing of Microalgae for Extraction of High-Value Products. Doctoral Dissertation.